Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: Label-free mass spectrometry data. List of statistically significant discovered proteins using LC-MS analysis. Proteins (in bold) were selected for further validation using ELISAs.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques: Mass Spectrometry, Biomarker Discovery, Variant Assay

Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: ELISA Data. Mean, SD, Area under the curve (AUC) and p -value for each of the new and standard proteins found in the two groups of patients compared.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: Logistic regression analysis data. List of different protein combinations used to establish the best model that can be used as a predictive panel for response to induction therapy containing bortezomib regime.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques:

Journal: Proteomics

Article Title: Identification, prioritization and evaluation of glycoproteins for aggressive prostate cancer using quantitative glycoproteomics and antibody-based assays on tissue specimens

doi: 10.1002/pmic.201200541

Figure Lengend Snippet: Standard Curve of ELISA assays for protein quantification of cartilage oligomeric matrix protein (COMP), periostin, membrane primary amine oxidase (VAP-1) and cathepsin L. A), dose-response curves for COMP. B), dose-response curve for periostin. C), dose-response curve for VAP-1. D), dose-response curve for cathepsin L.

Article Snippet: Materials Hydrazide resin and sodium periodate were from Bio-Rad (Hercules, CA); sequencing-grade trypsin and TMB reagent were from Promega (Madison, WI); PNGase F was from New England Biolabs (Ipswich, MA); C18 columns were from Waters (Milford, MA); Recombinant protein, capture and detection antibody of human cartilage oligomeric matrix protein (COMP), periostin, cathepsin L, clusterin and galectin-3 binding protein, streptavidin-HRP conjugates, ELISA plates and human VAP-1 Quantikine ELISA Kit were from R&D systems (Minneapolis, MN); All other chemicals were from Sigma-Aldrich (St. Louis, MO).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular Metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Selective deletion of LRP1 in GABAergic neurons causes metabolic disorders. ( A ) Body weight, ( B ) epididymal fat, ( C ) serum leptin, ( D ) serum FFA, ( E ) serum ApoJ, ( F ) blood glucose, ( G ) serum insulin, and ( H ) HOMA-IR were measured in LRP1 loxP/loxP and Vgat-Cre; LRP1 loxP/loxP male mice that were overnight fasted mice at 32 weeks of age. n = 12 for control, n = 18 for Vgat-Cre; LRP1 loxP/loxP . ( I ) Body weight, ( J ) blood glucose, ( K ) serum insulin, ( L ) serum leptin, and ( M ) serum ApoJ were measured in LRP1 loxP/loxP and Vgat-Cre; LRP1 loxP/loxP male mice that were overnight fasted at 16 weeks of age. n = 12 for control, n = 7 for Vgat-Cre; LRP1 loxP/loxP . All graphs represent means or individual values ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. LRP1 loxP/loxP by two-sided Student's t-test.

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Aβ 40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Aβ 42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques: Control

Journal: Molecular Metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Relationship of motor function, motor coordination, and spatial recognition memory with obesity-linked metabolic parameters. Correlation of body weight with ( A ) total distance traveled on the locomotion test, ( B ) latency to fall on the rotarod test, ( C ) total distance traveled, and ( D ) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum insulin with ( E ) latency to fall on the rotarod test, ( F ) total distance traveled, and ( G ) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum leptin with ( H ) latency to fall on the rotarod test, ( I ) total distance traveled, and ( J ) time spent in the novel arm on the spatial Y-maze test was evaluated. Correlation of serum ApoJ with ( K ) latency to fall on the rotarod test and (L) time spent in the novel arm on the spatial Y-maze test were evaluated. The P values were obtained by Pearson correlation analysis, and the r values indicate Pearson correlation coefficient. All correlations were analyzed from the data of LRP1 loxP/loxP and Vgat-Cre; LRP1 loxP/loxP male mice (32 weeks old).

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Aβ 40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Aβ 42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Molecular Metabolism

Article Title: LRP1 in GABAergic neurons is a key link between obesity and memory function

doi: 10.1016/j.molmet.2024.101941

Figure Lengend Snippet: Relationship between metabolic parameters and cognitive function. Correlation of body weight, serum leptin, serum insulin, and serum ApoJ with the data from acquisition ( A, C, E, G ) and reversal ( B, D, F, H ) water T-maze on day 1 was evaluated. The P values were obtained by Pearson correlation analysis and r values indicate the Pearson correlation coefficient. All correlations were analyzed from the data of LRP1 loxP/loxP and Vgat-Cre; LRP1 loxP/loxP male mice (32 weeks old).

Article Snippet: Serum levels of insulin (90080, Crystal Chem, Chicago, IL), leptin (90030, Crystal Chem, Chicago, IL), apolipoprotein J (ApoJ) (MCLU00, R&D Systems, Minneapolis, MN), Aβ 40 (KMB3481, Thermo Fisher Scientific Inc., Waltham, MA), Aβ 42 (KMB3441, Thermo Fisher Scientific Inc., Waltham, MA) and free fatty acids (FFA) (ab65341, Abcam, Waltham, MA) were measured by enzyme-linked immunosorbent assay (ELISA).

Techniques: