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clustergram module  (Shotgun Proteomics company)
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Shotgun Proteomics company clustergram module
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Module, supplied by Shotgun Proteomics company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clustergram representation  (Verlag GmbH)
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Verlag GmbH clustergram representation
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Representation, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clustergram/clustergram+representation/pm16358250-186-1-20
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clustergram representation - by Bioz Stars, 2026-10
90/100 stars
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Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Journal: Scientific Reports

Article Title: Mitofusin 1 silencing decreases the senescent associated secretory phenotype, promotes immune cell recruitment and delays melanoma tumor growth after chemotherapy

doi: 10.1038/s41598-024-51427-7

Figure Lengend Snippet: Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Article Snippet: Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Techniques: Transduction, Expressing, Quantitative RT-PCR, Control, Immunocytochemistry, shRNA, Activity Assay, Generated, Software