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Image Search Results
Journal: Artificial cells, nanomedicine, and biotechnology
Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.
doi: 10.1080/21691401.2019.1671434
Figure Lengend Snippet: Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage of caspase-3 and PARP, (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).
Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000),
Techniques: Expressing, CCK-8 Assay, Flow Cytometry, Western Blot
Journal: Artificial cells, nanomedicine, and biotechnology
Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.
doi: 10.1080/21691401.2019.1671434
Figure Lengend Snippet: Figure 2. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells. (A) NGR1 with various dosages ranged from 10 to 100 lM was utilized to treat RSC96 cells. (B) RSC96 cells were pre-treated with NGR1 and then stimulated by HG. Cell viability was examined by CCK-8 kit. 50 lM was applied as optimum dosage for use in following experiments. (C) Apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation and (G,H) expression of neurotrophic proteins were examined by flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).
Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000),
Techniques: CCK-8 Assay, Expressing, Flow Cytometry, Western Blot
Journal: Artificial cells, nanomedicine, and biotechnology
Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.
doi: 10.1080/21691401.2019.1671434
Figure Lengend Snippet: Figure 4. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells through miR-503. (A) miR-503 mimic and its respective control (NC mimic) were transfected into RSC96 cells. Transfection efficiency was examined by qRT-PCR. The transfected or non-transfected cells were then treated by NGR1, HG or both. (B) Cell viability, (C) apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation, and (G,H) expression of neuro- trophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).
Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000),
Techniques: Control, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Western Blot
Journal: PLoS ONE
Article Title: M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus
doi: 10.1371/journal.pone.0166966
Figure Lengend Snippet: Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved PARP-1 in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p < 0.01 when compared with Control, # p < 0.01 when compared with M30, $ p < 0.01 when compared with CORT + M30 groups, ! p < 0.01 when compared with Vehicle. For cleaved caspase 3 and cleaved PARP-1, *p < 0.001 when compared with Control, # p < 0.001 when compared with M30, $ p < 0.001 when compared with CORT + M30 groups, ! p < 0.001 when compared with Vehicle.
Article Snippet: Primary antibodies of SOD-2 (rabbit polyclonal, 1:1000), GPx-1 (goat polyclonal, 1:500), NFκB p65 (rabbit polyclonal, 1:250) and p50 (mouse monoclonal, 1:250), IκBα (mouse monoclonal, 1:500), TNF α (goat polyclonal, 1:80), IL-1β (rabbit polyclonal 1:100), IL-6 (goat polyclonal, 1:1000) and COX-2 (goat polyclonal, 1:100) were purchased from Santa Cruz Biotechnology, CA, USA; Synapsin 1 (rabbit polyclonal, 1:500) and Synaptophysin (rabbit polyclonal, 1:2000) were purchased from Novus Biologicals, USA; PSD95 (rabbit polyclonal 1:500), Cleaved Caspase 3 (rabbit polyclonal 1:500) was purchased from Cell Signaling Technology;
Techniques: Expressing