cleaved parp Search Results


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  • 96
    Millipore cleaved parp
    Effects of 200, 300, and 400 μ g/mL RVSE for 24 h on the <t>Bax:Bcl-2</t> ratio, and the cleaved caspase-3 and -9 and <t>PARP</t> levels in MCF-7 cells. Western blotting was performed for the determination of the relative protein levels of (a) Bax:Bcl-2 ratio, (b) cleaved caspase-9, (c) cleaved caspase-3, and (d) PARP and cleaved PARP. β -Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β -actin) were evaluated by densitometry. Data are means ± SD of three independent experiments. ∗P
    Cleaved Parp, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cell Signaling Technology Inc cleaved papr c parp
    Effects of 200, 300, and 400 μ g/mL RVSE for 24 h on the <t>Bax:Bcl-2</t> ratio, and the cleaved caspase-3 and -9 and <t>PARP</t> levels in MCF-7 cells. Western blotting was performed for the determination of the relative protein levels of (a) Bax:Bcl-2 ratio, (b) cleaved caspase-9, (c) cleaved caspase-3, and (d) PARP and cleaved PARP. β -Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β -actin) were evaluated by densitometry. Data are means ± SD of three independent experiments. ∗P
    Cleaved Papr C Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam cleaved parp c parp
    Effects of 200, 300, and 400 μ g/mL RVSE for 24 h on the <t>Bax:Bcl-2</t> ratio, and the cleaved caspase-3 and -9 and <t>PARP</t> levels in MCF-7 cells. Western blotting was performed for the determination of the relative protein levels of (a) Bax:Bcl-2 ratio, (b) cleaved caspase-9, (c) cleaved caspase-3, and (d) PARP and cleaved PARP. β -Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β -actin) were evaluated by densitometry. Data are means ± SD of three independent experiments. ∗P
    Cleaved Parp C Parp, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam cleaved parp
    LSF attenuates apoptosis in Aβ(1-42)-induced BV-2 cells. BV-2 cells were pretreated with 5 μM Aβ(1-42) for 12 h, then followed with an incubation of 0.12–0.48 mg⋅L -1 LSF for another 12 h. Cell lysates were then harvested and analyzed for Bax/Bcl-2 (A) , <t>Caspase-3</t> (C) , and <t>cleaved-PARP/PARP</t> (C) , respectively. Band intensities of Bax/Bcl-2 (B) , cleaved-PARP/PARP (D) and Caspase-3 (E) were quantified using Image J software and normalized to β-actin. Bars are representatives of three independent experiments. ∗∗∗ P
    Cleaved Parp, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc cleaved parp c parp
    Biomarkers analyses of bufalin-treated LNCaP xenograft tumors. A , H E-stained 3 rd -8 th ranked LNCaP xenograft tumors from ethanol vehicle- and bufalin-treated groups, respectively. Large eosinophilic areas without nuclei in vehicle group represent necrotic regions (marked N in middle panel). Scale bars, 50 micron. B , Representative IHC images of Ki-67 proliferation marker and apoptosis marker (c-caspase 3), <t>p-P53.Ser15</t> and AR in tumors from vehicle- or bufalin-treated mice. Black scale bars, 200 micron; Yellow scale bars, 100 micron. C , Western blot detection of <t>cleaved-PARP,</t> cleaved-caspase-3, p-P53.Ser15, AR and PSA in tumors from vehicle- or bufalin-treated mice. LNCaP cells exposed to 1 μM doxorubicin (a DNA damage drug) treatment for 24 h were used as a positive control for apoptosis (LN+Rx). PC3 extract and LNCaP extract (LN) were used as negative and positive control for AR and PSA, respectively. D , Representative images of SA-β-gal staining of cryo-sections of LNCaP xenograft tumors and the normal prostate of NSG SCID mice treated with vehicle or bufalin.
    Cleaved Parp C Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam anti cleaved parp
    Loss of c-Fos results in reduced taste cell differentiation and increased apoptosis. ( a ) Relative expression of indicated genes: c-Fos ( P =0.0056), Krt8 ( P =0.0252), Ki67 ( P =0.0202), Dusp14, Hmox1, Dcn and Ivl in CV/Fol taste cells from WT and c-Fos-KO mice. Statistical significance was determined using Student's t test. Error bars denote S.D. of three independent experiments. ( b ) <t>Immunohistochemical</t> analysis shows the expression level of <t>cleaved-PARP</t> in the CV taste cells of WT and c-Fos-KO mice, 14 days after tamoxifen treatment. ( c ) qRT-PCR analysis of taste cell type markers Glast (type I), Tas1R3 (type II) and Snap25 (type III). Error bars denote S.D. of three independent experiments. * P
    Anti Cleaved Parp, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Cell Signaling Technology Inc cleaved parp
    Increased hepatocyte apoptosis is seen after liver transplantation on immunohistochemistry staining of liver tissue. Photomicrographs show immunohistochemical staining of human liver sections for <t>M30</t> CytoDEATH and cleaved <t>PARP</t> at 200x magnification, indicating increased levels of these markers of apoptosis in post-liver transplant patients compared to healthy subjects (arrows indicate positive staining cells). Graphs show the relative staining of these apoptotic markers in the liver of each patient assessed by computerized image capture quantification with (A) M30 CytoDEATH and (B) cleaved PARP results expressed as mean proportional area stained within the given area and error bars represent SEM.
    Cleaved Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 7715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc anti cleaved prap c parp
    Curcumin induced apoptosis through activation of <t>caspase-3</t> followed by <t>PARP</t> degradation . KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). ( B ) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. ( C ) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.
    Anti Cleaved Prap C Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc cleaved c parp
    Curcumin induced apoptosis through activation of <t>caspase-3</t> followed by <t>PARP</t> degradation . KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). ( B ) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. ( C ) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.
    Cleaved C Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc anti cleaved parp c parp antibody
    Curcumin induced apoptosis through activation of <t>caspase-3</t> followed by <t>PARP</t> degradation . KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). ( B ) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. ( C ) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.
    Anti Cleaved Parp C Parp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson cleaved parp
    A plot for the Cleaved <t>Caspase</t> 3/Cleaved <t>PARP</t> double positive population at 48 hours measures the susceptibility of Hep3B and the resistance of Huh7 cells to 17AAG. Typical measurements of 24hr signaling degradation in response to 17AAG fail to correlate
    Cleaved Parp, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc c parp asp214
    A plot for the Cleaved <t>Caspase</t> 3/Cleaved <t>PARP</t> double positive population at 48 hours measures the susceptibility of Hep3B and the resistance of Huh7 cells to 17AAG. Typical measurements of 24hr signaling degradation in response to 17AAG fail to correlate
    C Parp Asp214, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology cleaved parp
    Expression of apoptosis-related proteins following sorbitol treatment. (A) Western blot analysis of caspase-3, cleaved capase-3, <t>PARP</t> and cleaved PARP. (B) Bar graphs represent the ratios of cleaved <t>capase-3/tubulin</t> and cleaved PARP/tubulin. * P
    Cleaved Parp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cell Signaling Technology Inc cleaved poly adp ribose polymerase c parp
    Expression of apoptosis-related proteins following sorbitol treatment. (A) Western blot analysis of caspase-3, cleaved capase-3, <t>PARP</t> and cleaved PARP. (B) Bar graphs represent the ratios of cleaved <t>capase-3/tubulin</t> and cleaved PARP/tubulin. * P
    Cleaved Poly Adp Ribose Polymerase C Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc cleaved parp 1
    M30 abrogated CORT-induced hippocampal apoptosis. Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved <t>PARP-1</t> in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p
    Cleaved Parp 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cell Signaling Technology Inc cleaved parp as214
    M30 abrogated CORT-induced hippocampal apoptosis. Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved <t>PARP-1</t> in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p
    Cleaved Parp As214, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Cell Signaling Technology Inc murine cleaved parp
    M30 abrogated CORT-induced hippocampal apoptosis. Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved <t>PARP-1</t> in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p
    Murine Cleaved Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology cleaved parp 1
    Molecular assays of CM apoptosis in the Gaq mice that underwent in-vivo imaging. Wt = wildtype mouse. A, Western blot for cleaved <t>PARP-1</t> and (B) for the control protein GAPDH. 9/10 of the Gaq mice had an EF
    Cleaved Parp 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology cleaved parp 1were
    Molecular assays of CM apoptosis in the Gaq mice that underwent in-vivo imaging. Wt = wildtype mouse. A, Western blot for cleaved <t>PARP-1</t> and (B) for the control protein GAPDH. 9/10 of the Gaq mice had an EF
    Cleaved Parp 1were, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cell Signaling Technology Inc cleaved parp cparp
    Molecular assays of CM apoptosis in the Gaq mice that underwent in-vivo imaging. Wt = wildtype mouse. A, Western blot for cleaved <t>PARP-1</t> and (B) for the control protein GAPDH. 9/10 of the Gaq mice had an EF
    Cleaved Parp Cparp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of 200, 300, and 400 μ g/mL RVSE for 24 h on the Bax:Bcl-2 ratio, and the cleaved caspase-3 and -9 and PARP levels in MCF-7 cells. Western blotting was performed for the determination of the relative protein levels of (a) Bax:Bcl-2 ratio, (b) cleaved caspase-9, (c) cleaved caspase-3, and (d) PARP and cleaved PARP. β -Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β -actin) were evaluated by densitometry. Data are means ± SD of three independent experiments. ∗P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Extract of Rhus verniciflua Stokes Induces p53-Mediated Apoptosis in MCF-7 Breast Cancer Cells

    doi: 10.1155/2019/9407340

    Figure Lengend Snippet: Effects of 200, 300, and 400 μ g/mL RVSE for 24 h on the Bax:Bcl-2 ratio, and the cleaved caspase-3 and -9 and PARP levels in MCF-7 cells. Western blotting was performed for the determination of the relative protein levels of (a) Bax:Bcl-2 ratio, (b) cleaved caspase-9, (c) cleaved caspase-3, and (d) PARP and cleaved PARP. β -Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β -actin) were evaluated by densitometry. Data are means ± SD of three independent experiments. ∗P

    Article Snippet: Monoclonal antibodies specific for Bax, Bcl-2, cleaved caspase-3 and -9, cleaved PARP, and PARP were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Western Blot

    LSF attenuates apoptosis in Aβ(1-42)-induced BV-2 cells. BV-2 cells were pretreated with 5 μM Aβ(1-42) for 12 h, then followed with an incubation of 0.12–0.48 mg⋅L -1 LSF for another 12 h. Cell lysates were then harvested and analyzed for Bax/Bcl-2 (A) , Caspase-3 (C) , and cleaved-PARP/PARP (C) , respectively. Band intensities of Bax/Bcl-2 (B) , cleaved-PARP/PARP (D) and Caspase-3 (E) were quantified using Image J software and normalized to β-actin. Bars are representatives of three independent experiments. ∗∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Lychee Seed Fraction Inhibits Aβ(1-42)-Induced Neuroinflammation in BV-2 Cells via NF-κB Signaling Pathway

    doi: 10.3389/fphar.2018.00380

    Figure Lengend Snippet: LSF attenuates apoptosis in Aβ(1-42)-induced BV-2 cells. BV-2 cells were pretreated with 5 μM Aβ(1-42) for 12 h, then followed with an incubation of 0.12–0.48 mg⋅L -1 LSF for another 12 h. Cell lysates were then harvested and analyzed for Bax/Bcl-2 (A) , Caspase-3 (C) , and cleaved-PARP/PARP (C) , respectively. Band intensities of Bax/Bcl-2 (B) , cleaved-PARP/PARP (D) and Caspase-3 (E) were quantified using Image J software and normalized to β-actin. Bars are representatives of three independent experiments. ∗∗∗ P

    Article Snippet: Caspase-3, cleaved-PARP and PARP antibodies were obtained from abcam (Cambridge, MA, United States).

    Techniques: Incubation, Software

    Biomarkers analyses of bufalin-treated LNCaP xenograft tumors. A , H E-stained 3 rd -8 th ranked LNCaP xenograft tumors from ethanol vehicle- and bufalin-treated groups, respectively. Large eosinophilic areas without nuclei in vehicle group represent necrotic regions (marked N in middle panel). Scale bars, 50 micron. B , Representative IHC images of Ki-67 proliferation marker and apoptosis marker (c-caspase 3), p-P53.Ser15 and AR in tumors from vehicle- or bufalin-treated mice. Black scale bars, 200 micron; Yellow scale bars, 100 micron. C , Western blot detection of cleaved-PARP, cleaved-caspase-3, p-P53.Ser15, AR and PSA in tumors from vehicle- or bufalin-treated mice. LNCaP cells exposed to 1 μM doxorubicin (a DNA damage drug) treatment for 24 h were used as a positive control for apoptosis (LN+Rx). PC3 extract and LNCaP extract (LN) were used as negative and positive control for AR and PSA, respectively. D , Representative images of SA-β-gal staining of cryo-sections of LNCaP xenograft tumors and the normal prostate of NSG SCID mice treated with vehicle or bufalin.

    Journal: Molecular cancer therapeutics

    Article Title: Role of P53-Senescence Induction in Suppression of LNCaP Prostate Cancer Growth by Cardiotonic Compound Bufalin

    doi: 10.1158/1535-7163.MCT-17-1296

    Figure Lengend Snippet: Biomarkers analyses of bufalin-treated LNCaP xenograft tumors. A , H E-stained 3 rd -8 th ranked LNCaP xenograft tumors from ethanol vehicle- and bufalin-treated groups, respectively. Large eosinophilic areas without nuclei in vehicle group represent necrotic regions (marked N in middle panel). Scale bars, 50 micron. B , Representative IHC images of Ki-67 proliferation marker and apoptosis marker (c-caspase 3), p-P53.Ser15 and AR in tumors from vehicle- or bufalin-treated mice. Black scale bars, 200 micron; Yellow scale bars, 100 micron. C , Western blot detection of cleaved-PARP, cleaved-caspase-3, p-P53.Ser15, AR and PSA in tumors from vehicle- or bufalin-treated mice. LNCaP cells exposed to 1 μM doxorubicin (a DNA damage drug) treatment for 24 h were used as a positive control for apoptosis (LN+Rx). PC3 extract and LNCaP extract (LN) were used as negative and positive control for AR and PSA, respectively. D , Representative images of SA-β-gal staining of cryo-sections of LNCaP xenograft tumors and the normal prostate of NSG SCID mice treated with vehicle or bufalin.

    Article Snippet: Antibodies for P53, phospho-P53 (Ser15), P21CIP1, cleaved-PARP (c-PARP), cleaved caspase-3, SRC-1, SRC-3, HK2, phospho-ATM (Ser1981), and phospho-H2A.X (Ser139) were obtained from Cell Signaling Technology (Danvers, MA).

    Techniques: Staining, Immunohistochemistry, Marker, Mouse Assay, Western Blot, Positive Control

    Impact of knocking down P53/P21CIP1 axis on bufalin induction of apoptosis and senescence in LNCaP cells. A , Western blot detection of P53 and P21CIP1 knocking down by siRNA and caspase-mediated PARP cleavage by bufalin after 24 h exposure. B , Western blot detection of P53 knocking down by siRNA 24 h after transfection and 5 days after initiation of bufalin treatment. C , Flow cytometry detection of SA-β-gal activity using C12FDG as substrate for scramble-RNA transfected vs. si- TP53 -knockdown cells exposed to bufalin for 5 days.

    Journal: Molecular cancer therapeutics

    Article Title: Role of P53-Senescence Induction in Suppression of LNCaP Prostate Cancer Growth by Cardiotonic Compound Bufalin

    doi: 10.1158/1535-7163.MCT-17-1296

    Figure Lengend Snippet: Impact of knocking down P53/P21CIP1 axis on bufalin induction of apoptosis and senescence in LNCaP cells. A , Western blot detection of P53 and P21CIP1 knocking down by siRNA and caspase-mediated PARP cleavage by bufalin after 24 h exposure. B , Western blot detection of P53 knocking down by siRNA 24 h after transfection and 5 days after initiation of bufalin treatment. C , Flow cytometry detection of SA-β-gal activity using C12FDG as substrate for scramble-RNA transfected vs. si- TP53 -knockdown cells exposed to bufalin for 5 days.

    Article Snippet: Antibodies for P53, phospho-P53 (Ser15), P21CIP1, cleaved-PARP (c-PARP), cleaved caspase-3, SRC-1, SRC-3, HK2, phospho-ATM (Ser1981), and phospho-H2A.X (Ser139) were obtained from Cell Signaling Technology (Danvers, MA).

    Techniques: Western Blot, Transfection, Flow Cytometry, Cytometry, Activity Assay

    Loss of c-Fos results in reduced taste cell differentiation and increased apoptosis. ( a ) Relative expression of indicated genes: c-Fos ( P =0.0056), Krt8 ( P =0.0252), Ki67 ( P =0.0202), Dusp14, Hmox1, Dcn and Ivl in CV/Fol taste cells from WT and c-Fos-KO mice. Statistical significance was determined using Student's t test. Error bars denote S.D. of three independent experiments. ( b ) Immunohistochemical analysis shows the expression level of cleaved-PARP in the CV taste cells of WT and c-Fos-KO mice, 14 days after tamoxifen treatment. ( c ) qRT-PCR analysis of taste cell type markers Glast (type I), Tas1R3 (type II) and Snap25 (type III). Error bars denote S.D. of three independent experiments. * P

    Journal: Cell Death & Disease

    Article Title: AP1 transcription factors are required to maintain the peripheral taste system

    doi: 10.1038/cddis.2016.343

    Figure Lengend Snippet: Loss of c-Fos results in reduced taste cell differentiation and increased apoptosis. ( a ) Relative expression of indicated genes: c-Fos ( P =0.0056), Krt8 ( P =0.0252), Ki67 ( P =0.0202), Dusp14, Hmox1, Dcn and Ivl in CV/Fol taste cells from WT and c-Fos-KO mice. Statistical significance was determined using Student's t test. Error bars denote S.D. of three independent experiments. ( b ) Immunohistochemical analysis shows the expression level of cleaved-PARP in the CV taste cells of WT and c-Fos-KO mice, 14 days after tamoxifen treatment. ( c ) qRT-PCR analysis of taste cell type markers Glast (type I), Tas1R3 (type II) and Snap25 (type III). Error bars denote S.D. of three independent experiments. * P

    Article Snippet: Immunohistochemistry Immunohistochemical analysis was performed as previously described., Antibodies were: anti-cleaved PARP (ab4830; 1:50), anti-c-Fos (ab7963; 1:50), anti-c-Jun (ab31419; 1:50), anti-AcH3 (ab1791; 1:200) from Abcam and anti-NTPdase2 (1:100).

    Techniques: Cell Differentiation, Expressing, Mouse Assay, Immunohistochemistry, Quantitative RT-PCR

    Increased hepatocyte apoptosis is seen after liver transplantation on immunohistochemistry staining of liver tissue. Photomicrographs show immunohistochemical staining of human liver sections for M30 CytoDEATH and cleaved PARP at 200x magnification, indicating increased levels of these markers of apoptosis in post-liver transplant patients compared to healthy subjects (arrows indicate positive staining cells). Graphs show the relative staining of these apoptotic markers in the liver of each patient assessed by computerized image capture quantification with (A) M30 CytoDEATH and (B) cleaved PARP results expressed as mean proportional area stained within the given area and error bars represent SEM.

    Journal: PLoS ONE

    Article Title: Effect of Immunosuppressive Agents on Hepatocyte Apoptosis Post-Liver Transplantation

    doi: 10.1371/journal.pone.0138522

    Figure Lengend Snippet: Increased hepatocyte apoptosis is seen after liver transplantation on immunohistochemistry staining of liver tissue. Photomicrographs show immunohistochemical staining of human liver sections for M30 CytoDEATH and cleaved PARP at 200x magnification, indicating increased levels of these markers of apoptosis in post-liver transplant patients compared to healthy subjects (arrows indicate positive staining cells). Graphs show the relative staining of these apoptotic markers in the liver of each patient assessed by computerized image capture quantification with (A) M30 CytoDEATH and (B) cleaved PARP results expressed as mean proportional area stained within the given area and error bars represent SEM.

    Article Snippet: Sections were examined for cleaved cytokeratin 18 (M30 CytoDEATH) and cleaved PARP, both markers of apoptosis.

    Techniques: Transplantation Assay, Immunohistochemistry, Staining

    Curcumin induced apoptosis through activation of caspase-3 followed by PARP degradation . KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). ( B ) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. ( C ) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.

    Journal: Journal of Translational Medicine

    Article Title: Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells

    doi: 10.1186/1479-5876-9-71

    Figure Lengend Snippet: Curcumin induced apoptosis through activation of caspase-3 followed by PARP degradation . KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). ( B ) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. ( C ) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.

    Article Snippet: Anti-cleaved PARP, cleaved caspase-3, and Bcl-2 antibodies were purchased from Cell Signaling Technologies (Beverly, MA, USA).

    Techniques: Activation Assay, Incubation, Staining, Light Microscopy, Western Blot

    A plot for the Cleaved Caspase 3/Cleaved PARP double positive population at 48 hours measures the susceptibility of Hep3B and the resistance of Huh7 cells to 17AAG. Typical measurements of 24hr signaling degradation in response to 17AAG fail to correlate

    Journal:

    Article Title: Three-Kinase Inhibitor Combination Recreates Multi-Pathway Effects of a Geldanamycin Analog on Hepatocellular Carcinoma Cell Death

    doi: 10.1158/1535-7163.MCT-08-1203

    Figure Lengend Snippet: A plot for the Cleaved Caspase 3/Cleaved PARP double positive population at 48 hours measures the susceptibility of Hep3B and the resistance of Huh7 cells to 17AAG. Typical measurements of 24hr signaling degradation in response to 17AAG fail to correlate

    Article Snippet: Cells were stained with antibodies for Cleaved (Activated) Caspase 3 (BD Pharmingen, San Jose, CA, cat# 559565) and Cleaved PARP (BD Pharmingen cat# 51–9000017) at a dilution of 1:300 (in PBS-0.1%Tween20–1%BSA).

    Techniques:

    Expression of apoptosis-related proteins following sorbitol treatment. (A) Western blot analysis of caspase-3, cleaved capase-3, PARP and cleaved PARP. (B) Bar graphs represent the ratios of cleaved capase-3/tubulin and cleaved PARP/tubulin. * P

    Journal: Oncology Letters

    Article Title: Sorbitol induces apoptosis of human colorectal cancer cells via p38 MAPK signal transduction

    doi: 10.3892/ol.2014.1994

    Figure Lengend Snippet: Expression of apoptosis-related proteins following sorbitol treatment. (A) Western blot analysis of caspase-3, cleaved capase-3, PARP and cleaved PARP. (B) Bar graphs represent the ratios of cleaved capase-3/tubulin and cleaved PARP/tubulin. * P

    Article Snippet: Reagents and antibodies Sorbitol and MTT were employed (Sigma, St. Louis, MO, USA) as well as antibodies against tubulin, poly (ADP-ribose) polymerase (PARP), cleaved PARP (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), capase-3, cleaved capase-3, Bax, Bcl-2, cytochrome c (Abcam, Cambridge, UK), p38 and p-p38 (BD Pharmingen, San Diego, CA, USA).

    Techniques: Expressing, Western Blot

    M30 abrogated CORT-induced hippocampal apoptosis. Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved PARP-1 in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p

    Journal: PLoS ONE

    Article Title: M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus

    doi: 10.1371/journal.pone.0166966

    Figure Lengend Snippet: M30 abrogated CORT-induced hippocampal apoptosis. Levels of protein expression of (A) Bcl-2, (B) Cleaved Caspase 3 and (C) Cleaved PARP-1 in the hippocampus of the control, CORT-treated (CORT), M30-treated (M30), CORT and M30 co-treated (CORT+M30) or vehicle groups are summarized. β-actin was an internal control. Data from each group were expressed as mean ± SEM (n = 8). Statistical comparisons between groups were performed using the One way Anova followed by Tukey post hoc test to detect differences in all groups. For Bcl-2, *p

    Article Snippet: Primary antibodies of SOD-2 (rabbit polyclonal, 1:1000), GPx-1 (goat polyclonal, 1:500), NFκB p65 (rabbit polyclonal, 1:250) and p50 (mouse monoclonal, 1:250), IκBα (mouse monoclonal, 1:500), TNF α (goat polyclonal, 1:80), IL-1β (rabbit polyclonal 1:100), IL-6 (goat polyclonal, 1:1000) and COX-2 (goat polyclonal, 1:100) were purchased from Santa Cruz Biotechnology, CA, USA; Synapsin 1 (rabbit polyclonal, 1:500) and Synaptophysin (rabbit polyclonal, 1:2000) were purchased from Novus Biologicals, USA; PSD95 (rabbit polyclonal 1:500), Cleaved Caspase 3 (rabbit polyclonal 1:500) was purchased from Cell Signaling Technology; Cleaved PARP-1 (rabbit polyclonal, 1:2000) was purchased from Bioworld Technology; IDO-1 (rabbit polyclonal, 1:250) was purchased from antibodies-online (ABIN1714836).

    Techniques: Expressing

    Molecular assays of CM apoptosis in the Gaq mice that underwent in-vivo imaging. Wt = wildtype mouse. A, Western blot for cleaved PARP-1 and (B) for the control protein GAPDH. 9/10 of the Gaq mice had an EF

    Journal:

    Article Title: Molecular MRI Detects Low levels of Cardiomyocyte Apoptosis in a Transgenic Model of Chronic Heart Failure

    doi: 10.1161/CIRCIMAGING.109.863779

    Figure Lengend Snippet: Molecular assays of CM apoptosis in the Gaq mice that underwent in-vivo imaging. Wt = wildtype mouse. A, Western blot for cleaved PARP-1 and (B) for the control protein GAPDH. 9/10 of the Gaq mice had an EF

    Article Snippet: It should also be noted that while levels of cleaved PARP-1 largely represent its cleavage by caspase-3, recent reports suggest that autophagy can also result in the cleavage of PARP-1.

    Techniques: Mouse Assay, In Vivo Imaging, Western Blot

    Cine MRI of postpartum Gaq mice. Images at the midventricular level are shown: Panels A, B: End diastolic and systolic images in a mouse with high levels of PARP-1 and an EF of 51%. C, D: End diastolic and systolic images in a mouse with low levels of

    Journal:

    Article Title: Molecular MRI Detects Low levels of Cardiomyocyte Apoptosis in a Transgenic Model of Chronic Heart Failure

    doi: 10.1161/CIRCIMAGING.109.863779

    Figure Lengend Snippet: Cine MRI of postpartum Gaq mice. Images at the midventricular level are shown: Panels A, B: End diastolic and systolic images in a mouse with high levels of PARP-1 and an EF of 51%. C, D: End diastolic and systolic images in a mouse with low levels of

    Article Snippet: It should also be noted that while levels of cleaved PARP-1 largely represent its cleavage by caspase-3, recent reports suggest that autophagy can also result in the cleavage of PARP-1.

    Techniques: Magnetic Resonance Imaging, Mouse Assay