Journal: Journal of Neuroinflammation

Article Title: Intrauterine inflammation and postnatal intravenous dopamine alter the neurovascular unit in preterm newborn lambs

doi: 10.1186/s12974-024-03137-0

Figure Lengend Snippet: Genes of interest

Article Snippet: , Claudin-1 , CLDN1 , Oa03217991_m1.

Techniques:

Journal: Scientific Reports

Article Title: AOH induces oxidative stress and DNA damage in ovarian cancer cells via modulation of GPER1 and HIF1α/PI3K/CLDNs signaling pathway

doi: 10.1038/s41598-025-26042-9

Figure Lengend Snippet: AOH modulates the expression of HIF1α , Akt and claudins . ( a ) HIF1α gene expression: Relative expression of HIF1α. ( b ) CLDN1, CLDN3 and CLDN3 protein expression: Western Blot analysis of CLDN1, CLDN3 and CLDN3, the representative results. ( c ) CLDN1, CLDN3 and CLDN3 gene expression: Relative expression of CLDN1. CLDN3 and CLDN4 . ( d ) pAkt, Akt and PI3K protein expression: Western Blot analysis of pAkt, Akt and PI3K, the representative results. ( e ) pp44/42 and p44/42 protein expression: Western Blot analysis of pp44/42 and p44/42, the representative results. p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as compared to the control. AOH alternariol. GPER1 G protein-coupled estrogen receptor 1. G15 selective GPER1 receptor antagonist. G1 selective GPER1 agonist, Cnt control (non-treated cells), Vin vinculin, HIF1α hypoxia-inducible factor 1-alpha, CLDN1 claudin 1, CLDN3 claudin 3, CLDN4 claudin 4 , p44/42 extracellular signal-regulated kinases, phospho-p44/42 phospho-extracellular signal-regulated kinases, Akt protein kinase B, pAkt phospho-protein kinase B, PI3K phosphoinositide 3-kinase.

Article Snippet: Akt (#9272), phospho-Akt (Ser473) (#4060), PARP1 (#46,011), SOD1 (#4266), SOD2 (#13,141), pH2AX (#9718), CLDN1 (#13,995), CLDN3 (#83,609), PI3K (#11,889), p44/42 (#4695), phospho-p44/42 (#4370), CLDN4 (STJ114778), GPER1 (STJ192629) (St John’s Laboratory Ltd, University Way, London) antibodies were used according to the manufacturer’s recommendations.

Techniques: Expressing, Gene Expression, Western Blot, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 ( Cldn10 ) in Cytodifferentiation of Stratum Intermedium

doi: 10.3389/fcell.2020.595593

Figure Lengend Snippet: Expression patterns of Cldn10 during tooth development. (A) Genomic locus of mouse Cldn10 in Cap Analysis Gene Expression (CAGE). The CAGE dataset from tooth germs showed a high peak at the Cldn10b transcription start site. The red arrowhead indicates the CAGE peak. (B) Schematic summary of Cldn10 isoforms in the rat genomic locus. Red arrowheads indicate the locations of intron-spanning primers used in quantitative reverse transcription polymerase chain reaction (RT-qPCR). (C) Expression levels of Cldn10 mRNA isoforms a and b in postnatal (P) day P7 rat tissues ( n = 3). The relative mRNA expression was standardized to Gapdh expression. Error bars represent S.D. SMG , submandibular gland; PTH , parathyroid. (D) mRNA expression levels of Cldn10 , SI cell markers Alpl and Notch1 , and ameloblast marker Ambn at different developmental stages of mouse tooth germ ( n = 3). The relative mRNA expression was standardized to Gapdh expression. Error bars represent S.D.

Article Snippet: For the transfection experiments, SF2 cells were transfected with the Cldn10 -transcript variant b expression vector pCMV6-Entry- Cldn10b (OriGene Technologies, Rockville, MD, United States).

Techniques: Expressing, Gene Expression, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 ( Cldn10 ) in Cytodifferentiation of Stratum Intermedium

doi: 10.3389/fcell.2020.595593

Figure Lengend Snippet: Spatiotemporal expression pattern of claudin-10 during tooth development. (A) Immunofluorescence staining of claudin-10 in postnatal (P) day P1 mouse lower incisors. Dashed white boxes indicate the area enlarged in the lower panel. Scale bar, 100 μm. Lower panel: enlarged image shown in the upper panel. Scale bars, 50 μm. (B) Immunofluorescence staining of claudin-10 in embryonic (E) day E14, E16, P1, and P3 mouse molar tooth germs. Green , claudin-10; blue , DAPI (4’,6-diamidino-2-phenylindol); iee , inner enamel epithelium; si , stratum intermedium; am , ameloblast; od , odontoblast; de , dental epithelium; dm , dental mesenchyme. Dashed white boxes indicate the area enlarged in the right panel. Scale bars, 100 μm.

Article Snippet: For the transfection experiments, SF2 cells were transfected with the Cldn10 -transcript variant b expression vector pCMV6-Entry- Cldn10b (OriGene Technologies, Rockville, MD, United States).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 ( Cldn10 ) in Cytodifferentiation of Stratum Intermedium

doi: 10.3389/fcell.2020.595593

Figure Lengend Snippet: Overexpression of claudin-10b enhances differentiation of rat incisor-derived dental epithelial SF2 cells into the stratum intermedium (SI) cell lineage. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of Cldn1 , Cldn10 , Krt14 , and Vim expression in SF2 cells and a mouse dental papilla (mDP) mesenchymal cell line. Krt14 and Vim were used as representative epithelial and mesenchymal marker genes, respectively. (B) Time-course analysis of Cldn10 , Alpl , and Notch1 expression in SF2 cells using quantitative RT-qPCR ( n = 3). Relative mRNA expression was standardized to Gapdh expression. Error bars represent S.D. (C) SF2 cells were transfected with a mock control vector or a Cldn10b expression vector. Western blot analysis showed high expression levels of claudin-10 protein in Cldn10b- overexpressing SF2 cells. (D) Immunofluorescence of Claudin-10 in mock-transfected and Cldn10b- overexpressing SF2 cells. Upper panel: Green , claudin-10; blue , DAPI. Lower panel: merged image with phase contrast from upper panel. Scale bars, 10 μm. (E) Immunofluorescence of ZO-1 in mock-transfected and Cldn10b- overexpressing SF2 cells. Upper panel: Green , ZO-1; blue , DAPI. Lower panel: merged image with phase contrast from upper panel. Scale bars, 10 μm. (F) Cell Counting Kit-8 (CCK-8) assay of mock-transfected and Cldn10b -transfected cells revealed similar proliferation rates ( n = 3). Error bars represent S.D. ns (not significant), p > 0.05 with two-way ANOVA. (G) Expression of Alpl , Notch1 , AmeloD , and Ambn in Cldn10b -overexpressing SF2 cells ( n = 3). Error bars represent S.D. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 with two-tailed t -test.

Article Snippet: For the transfection experiments, SF2 cells were transfected with the Cldn10 -transcript variant b expression vector pCMV6-Entry- Cldn10b (OriGene Technologies, Rockville, MD, United States).

Techniques: Over Expression, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Quantitative RT-PCR, Transfection, Control, Plasmid Preparation, Western Blot, Immunofluorescence, Cell Counting, CCK-8 Assay, Two Tailed Test