Journal: Cell Research

Article Title: Genomic and transcriptomic dynamics in the stepwise progression of lung adenocarcinoma

doi: 10.1038/s41422-025-01200-w

Figure Lengend Snippet: a Study design of in vitro and in vivo experiments. b Western blot analysis of phosphorylated and total Mek1 and Erk in Trp53 −/− –ATII organoids. NC negative control group, WT group overexpressing WT MAP2K1 , MT group overexpressing MAP2K1 ΔE102−I103 . c Morphological changes (top), H&E staining (second row), and IHC of EpCAM (third row) and TTF-1 (bottom) in Trp53 −/− –ATII organoids after 5 days of culture. Scale bar, 50 µm. d Relative viability of organoids on days 1, 3, and 5. n = 3 biological replicates. e Relative organoid area of organoids on day 5. f – h Growth curve ( f ), end point illustration ( g , scale bar, 1 cm), and tumor weight ( h ) of Trp53 −/− ( n = 4), Trp53 −/− ; MAP2K1 WT ( n = 4), and Trp53 −/− ; MAP2K1 MT ( n = 4) allografts. i H&E staining and IHC of TTF-1, KRT7, and EpCAM of the Trp53 −/− ; MAP2K1 MT organoid allograft at the experiment endpoint (day 48). Scale bar, 50 µm. j Efficacy of SM1-71, an inhibitor of MAP2K1, in treating MAP2K1 MT , MAP2K1 WT , and negative-control Ba/F3 cells. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The following primary antibodies were used: EpCam (Abcam, Cat# ab71916), TTF-1 (Abcam, Cat# ab76013), and KRT7 (Proteintech, Cat# 17513-1-AP).

Techniques: In Vitro, In Vivo, Western Blot, Negative Control, Staining

Journal: bioRxiv

Article Title: HuPSA and MoPSA Atlases Revealed Novel Cell Populations and Lineage Plasticity at Single-Cell Resolution in Prostate Cancer

doi: 10.1101/2023.08.11.553009

Figure Lengend Snippet: KRT7 gene expression represents unique populations in HuPSA and MoPSA. Feature plots of KRT7 (Krt7 as mouse gene symbol) gene expression showed the KRT7 expressed in KRT7 cluster in HuPSA (left) and Krt7 cluster in MoPSA (right) and some basal epithelium cells in both datasets (A). Geyser plots (scatter plots) quantified genes expression in HuPSA (left) and MoPSA (right). The KRT7/ Krt7 populations were highlighted by red rectangle.

Article Snippet: Primary antibodies include KRT7 (abclonal, A4357), MMP7 (abclonal, A0695), CK5 (BioLegend, 904801), CK8 (BioLegend,904801).

Techniques: Gene Expression, Expressing

Journal: bioRxiv

Article Title: HuPSA and MoPSA Atlases Revealed Novel Cell Populations and Lineage Plasticity at Single-Cell Resolution in Prostate Cancer

doi: 10.1101/2023.08.11.553009

Figure Lengend Snippet: Characterizing KRT7 populations in human and mouse. In human benign prostatic hyperplasia (BPH) tissue, Krt7 was highly expressed in basal epithelium cells but not in luminal epithelium cells (A). Prostate cancer cells showed negative Krt7 expression (B). In some cases, scattered populations (C) or major populations (D) exhibited high Krt7 expression. Advanced prostate cancer cell line xenograft tissues showed detectable Krt7 expression, with high expression in subpopulations of 22RV1 and PC3 cells, and moderate expression in the majority of DU145 cells (E-K). KRT7 mRNA expression in cultured cells correlated roughly with the IHC staining, with high levels in PC3 and DU145 cells, and low levels in 22RV1 and H660 cells (F). The Krt7 and AdPCa populations from “GSE210358” study were isolated from MoPSA and visualized by UMAP as a dim plot (M). The same UMAP plot was labeled with treatment information consisting of intact, castration in 1-28 days and regeneration in 1-14days (N). In wild-type (WT) mouse prostate, Krt7 expression was detected in sub populations both basal and luminal epithelium cells. The normal Krt7+ luminal epithelium cells exhibited resistance to castration (CX). Over the treatment time scale, in intact mouse prostate, Krt7 expression was rarely detected in luminal epithelium cells. However, Krt7+ populations were enriched in CX 7 days and CX 21 days samples. After testosterone treatment, along with luminal epithelium regeneration, the percentage of Krt7+ cells decreased. In transgenic adenocarcinoma of the mouse prostate (TRAMP) tissues, intact mouse tissue showed Krt7 expression in a subpopulation of prostatic intraepithelial neoplasia (PIN) cells. After castration, Krt7 was detected in all PIN cells but not in neuroendocrine prostate cancer cells (O-V).

Article Snippet: Primary antibodies include KRT7 (abclonal, A4357), MMP7 (abclonal, A0695), CK5 (BioLegend, 904801), CK8 (BioLegend,904801).

Techniques: Expressing, Cell Culture, Immunohistochemistry, Isolation, Labeling, Transgenic Assay