Journal: Advanced Science

Article Title: The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer Cells

doi: 10.1002/advs.202512652

Figure Lengend Snippet: APC11 depletion promotes CUL5 neddylation but stabilizes integrin β1. a) Immunoblot of indicated proteins in PLC/PRF/5, Hep3B, and PANC‐1 cells transfected with siRNA targeting APC11 or with a scrambled control siRNA. b) Co‐IP of transfected FLAG‐CUL5 and endogenous CAND1, COPS5, UBE2F or SAG in HEK293 cells transfected with indicated siRNA oligos. c) Co‐IP of endogenous CUL5 with UBE2F or APC11 in A549 cells transfected with indicated siRNA oligos. d) Immunoblot of CUL5 in A549 and PLC/PRF/5 cells transfected with indicated siRNA oligos. e) The stability of SOCS3 and integrin β1 in A549 and MIA PaCa‐2 cells upon APC11 knockdown. A549 and MIA PaCa‐2 cells were transfected with indicated siRNA oligos for 72 h and then treated with 100 µg mL −1 CHX for the indicated time periods, followed by IB analysis. Densitometry quantifications were performed with ImageJ, and the decay curves are shown (right). Data are presented as mean ± SEM, n = 3. For statistical analysis, significances were determined by Student's t ‐test. * p < 0.05, ** p < 0.01, *** p < 0.001. f) Diagram illustrating how APC11 depletion promotes CUL5 neddylation and leads to integrin β1 accumulation.

Article Snippet: [ ] The following antibodies were used: ACTIN (Sigma–Aldrich, A5441), FLAG (Sigma–Aldrich, F1804), FLAG (Sigma–Aldrich, F7425), GFP (Santa Cruz, sc‐9996), MYC (Santa Cruz, sc‐789), APC2 (Cell Signaling Technology, 12301), APC8 (Cell Signaling Technology, 15100), APC10 (Cell Signaling Technology, 14807), APC11 (Cell Signaling Technology, 14090), APC11 (Santa Cruz, sc‐517142), SAG (Proteintech, 11905‐1‐AP), RBX1 (Proteintech, 14895‐1‐AP), CUL1 (Santa Cruz, sc‐11384), CUL5 (Abcam, ab184177), CUL5 (Santa Cruz, sc‐373822), CAND1 (Abcam, ab183748), integrin β1 (Cell Signaling Technology, 34 971), p‐SRC (Y416) (Cell Signaling Technology, 6943), ATM (Cell Signaling Technology, 2873), Cyclin B1 (Cell Signaling Technology, 4138), RPB1 (Cell Signaling Technology, 14958), EGFR (Cell Signaling Technology, 4267), DEPTOR (Cell Signaling Technology, 11 816), NOXA (Millipore, OP180), UBE2F (Proteintech, 17056‐1‐AP), COPS5 (Cell Signaling Technology, 6895), SOCS3 (Proteintech, 14025‐1‐AP), LC3 (Cell Signaling Technology, 2775), securin (Cell Signaling Technology, 13445), ITCH (Proteintech, 20920‐1‐AP), Cyclin E1 (Cell Signaling Technology, 20808), p‐H3 (Cell Signaling Technology, 3377), and p‐Aurora A/B/C (Cell Signaling Technology, 2914), Aurora A (Cell Signaling Technology, 14 475), Aurora B (Abcam, ab2254), and NEDD8 (Abcam, ab81264).

Techniques: Western Blot, Transfection, Control, Co-Immunoprecipitation Assay, Knockdown

Journal: Journal of Agricultural and Food Chemistry

Article Title: Mapping Royal Jelly’s Bioactive Profile by Liquid Chromatography Coupled to Trapped Ion Mobility Spectrometry and High-Resolution Mass Spectrometry

doi: 10.1021/acs.jafc.5c04904

Figure Lengend Snippet: Pareto chart for (A) Apigenin, (B) Chrysin, (C) Pinobanksin, (D) Quercetin, (E) Scopoletin, and (F) 2- cis ,4- trans Abscisic acid.

Article Snippet: Kaempferol (purity ≥98%) and 2- cis ,4- trans -abscisic acid (purity ≥98%) were purchased from Santa Cruz-Biotechnology (Santa Cruz, CA), while verbascoside (purity ≥99%) was purchased from HWI pharma services GmbH (Frankfurt, Germany).

Techniques: