Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: Circulating expression of Hsa_circRNA_102893 contributes to early gestational diabetes mellitus detection

doi: 10.1038/s41598-020-76013-5

Figure Lengend Snippet: Circulating hsa_circRNA_102893 expressed in the plasma of pregnant women with GDM in comparison with healthy pregnant women (control). ( A ) Relative expression levels of hsa_circRNA_102893 with their corresponding P values (Paired t-test) in the training set and test set. Scatter plots show relative expression levels. The orange boxes and the horizontal bars represent the mean and the standard error, respectively. ( B ) ROC curves of hsa_circRNA_102893 and the AUC, sensitivity, specificity and cutoff point in the training set and test set. Red point is corresponding to Youden index.

Article Snippet: CircRNA microarray hybridization was performed according to the manufacturer's protocol of Arraystar Human CircRNA Array (6 × 7 K, Arraystar, Rockville, Maryland, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Circulating expression of Hsa_circRNA_102893 contributes to early gestational diabetes mellitus detection

doi: 10.1038/s41598-020-76013-5

Figure Lengend Snippet: Diagnostic performance of hsa_circRNA_102893 for the prediction of pregnant women with GDM and healthy pregnant women (Control).

Article Snippet: CircRNA microarray hybridization was performed according to the manufacturer's protocol of Arraystar Human CircRNA Array (6 × 7 K, Arraystar, Rockville, Maryland, USA).

Techniques: Diagnostic Assay

Journal: Cell Cycle

Article Title: Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer

doi: 10.1080/15384101.2017.1380135

Figure Lengend Snippet: Heat map of the circRNA and mRNA profiles in GC tissues versus paired normal gastric mucosa tissues. Red indicates high relative expression, and green indicates low relative expression. The expression of mRNA (A) and circRNA (B) were hierarchically clustered on the y-axis, and the tissue samples are hierarchically clustered on the x-axis (Fold change ≧ 2, p < 0.05). Expression levels are presented in red and green, indicating upregulated and downregulated RNAs, respectively. Numbers marked with T and N are from five paired gastric cancer tissues and paired adjacent normal tissues, respectively.

Article Snippet: The labeled cRNAs were hybridized onto the CapitalBio Technology Human CircRNA Microarray v2.0 (V2.0,4 × 180K) and mRNA Human Gene Expression Microarray (V4.0,4 × 180K), including the global profiling of 170,340 circRNAs and 34,235 coding transcripts (from 21,901 genes).

Techniques: Expressing

Journal: Cell Cycle

Article Title: Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer

doi: 10.1080/15384101.2017.1380135

Figure Lengend Snippet: Co-expression network in GC tissues and adjacent normal tissues. The co-expression network consists of 204 circRNAs and 169 mRNAs. (A) GC tissues; (B) Normal tissues. A round node represents a protein-coding gene, and a triangular node represents a circRNA. Solid lines between two nodes indicate positively-correlated interactions between genes, and dashed lines indicate negatively-correlated interactions. Structural cohesion levels are presented in different colors; red indicates a high cohesion level.

Article Snippet: The labeled cRNAs were hybridized onto the CapitalBio Technology Human CircRNA Microarray v2.0 (V2.0,4 × 180K) and mRNA Human Gene Expression Microarray (V4.0,4 × 180K), including the global profiling of 170,340 circRNAs and 34,235 coding transcripts (from 21,901 genes).

Techniques: Expressing

Journal: Cell Cycle

Article Title: Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer

doi: 10.1080/15384101.2017.1380135

Figure Lengend Snippet: Top 3 Critical circRNAs.

Article Snippet: The labeled cRNAs were hybridized onto the CapitalBio Technology Human CircRNA Microarray v2.0 (V2.0,4 × 180K) and mRNA Human Gene Expression Microarray (V4.0,4 × 180K), including the global profiling of 170,340 circRNAs and 34,235 coding transcripts (from 21,901 genes).

Techniques:

Journal: Cell Cycle

Article Title: Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer

doi: 10.1080/15384101.2017.1380135

Figure Lengend Snippet: siRNAs targeted at circRNA backspliced junction sequences.

Article Snippet: The labeled cRNAs were hybridized onto the CapitalBio Technology Human CircRNA Microarray v2.0 (V2.0,4 × 180K) and mRNA Human Gene Expression Microarray (V4.0,4 × 180K), including the global profiling of 170,340 circRNAs and 34,235 coding transcripts (from 21,901 genes).

Techniques: