Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in the plasma of ESCC patients.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Clinical Proteomics

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in ESCC patients. (A) The scatter plot of differentially expressed circRNAs. Green lines represent fold change of 2.0. (B) Volcano plot of differentially expressed circRNAs. The green vertical lines correspond to a fold change of 2.0, and the green horizontal line corresponds to a P -value of 0.05. (C) Hierarchical clustering of differentially expressed circRNAs. Each column represents a sample and each row represents a circRNA. The color reflects the expression level of circRNAs and changes from red (high) to black (medium) to green (low). (D) Bar plot shows the distributions of the differentially expressed circRNAs in human chromosomes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: CircRNA–miRNA–mRNA interaction network in ESCC. CircRNAs are represented by diamonds; miRNAs are shown as triangles; the DEGs are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA; ESCC, esophageal squamous cell carcinoma; DEGs, differentially expressed genes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Sub-network of circRNAs, miRNAs, and hub genes. circRNAs are represented by diamonds; miRNAs are shown as triangles; hub genes are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Blocking Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: Data in Brief

Article Title: Microarray expression profile of circular RNAs in lung tissues from rats with lipopolysaccharide-induced acute respiratory distress syndrome

doi: 10.1016/j.dib.2017.10.018

Figure Lengend Snippet: Experiment workflow of microarray expression profile of circular RNAs.

Article Snippet: The circRNA expression microarray slides were incubated for 17 hours at 65 °C in an Agilent Hybridization Oven.

Techniques: Microarray, Expressing

Journal: Data in Brief

Article Title: Microarray expression profile of circular RNAs in lung tissues from rats with lipopolysaccharide-induced acute respiratory distress syndrome

doi: 10.1016/j.dib.2017.10.018

Figure Lengend Snippet:

Article Snippet: The circRNA expression microarray slides were incubated for 17 hours at 65 °C in an Agilent Hybridization Oven.

Techniques: Microarray, Expressing