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Image Search Results
Journal: Oncogene
Article Title: Circular RNAs add diversity to androgen receptor isoform repertoire in castration-resistant prostate cancer
doi: 10.1038/s41388-019-0947-7
Figure Lengend Snippet: (A) Pearson correlation analysis showing strong positive correlations between circARs with AR-FL/-Vs. The blue gradient bar indicates the the scale of correlation coefficiency scores. (B) Schematic diagrams showing that the splice junctions that are specific to the most highly concordant circAR/AR-V pairs, circAR-E3/AR-V9, circAR-E2In1/AR-V3, and circAR-E2In2/AR-V3, are in close proximity.
Article Snippet: The custom-designed
Techniques:
Journal: Oncogene
Article Title: Circular RNAs add diversity to androgen receptor isoform repertoire in castration-resistant prostate cancer
doi: 10.1038/s41388-019-0947-7
Figure Lengend Snippet: (A) RT-qPCR analysis of nuclear and cytoplasmic RNA fractions showing predominant cytoplasmic localization of circARs in VCaP cells. Cytoplasmic-localized RPL30 and nuclear-localized 45S were used for comparison. The cytoplasmic to nuclear RNA ratios are plotted as ΔCt between the cytoplasmic and nuclear Ct values. (B & C) Two-dimensional (B) and three-dimensional (C) confocal fluorescence microscopy of circAR-E3 RISH showing predominant cytoplasmic localization of circAR-E3 in a LuCaP 35CR tumor. DAPI was used for nuclear staining. 3D images were composited from z-stack analysis.
Article Snippet: The custom-designed
Techniques: Quantitative RT-PCR, Comparison, Fluorescence, Microscopy, Staining
Journal: Oncogene
Article Title: Circular RNAs add diversity to androgen receptor isoform repertoire in castration-resistant prostate cancer
doi: 10.1038/s41388-019-0947-7
Figure Lengend Snippet: (A) circAR-E3 is upregulated during castration-resistant progression of LuCaP PDX models. RISH was conducted on 3 different xenograft passages per PDX model. (B) Detection of circAR-E2 in plasma samples from 7 mCRPC patients. RT-PCR analysis was performed on RNA samples isolated from 0.25 ml of patient plasma. circGSE1 was used as a control circRNA that is present in the plasma.
Article Snippet: The custom-designed
Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation