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  • 92
    Millipore affinity purified rabbit anti cht1 polyclonal antibody ab5966
    Affinity Purified Rabbit Anti Cht1 Polyclonal Antibody Ab5966, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore cht1
    Distribution of <t>CHT1</t> in the MP of colon. Notes: Immunofluorescence for CHT1, PGP9.5, and merged picture on whole-mount preparations of colonic MP from control ( A ), WAS ( B ), and WAS + MKC-231 rats ( C ) (200×) was displayed. The percentage of CHT1-positive neurons in the MP of WAS is significantly higher than that in the control group. In addition, the percentage of CHT1-positive neurons in the WAS + MKC-231 group is enhanced compared to that in the WAS group. Bars = 100 µm; n of each group = 6. Abbreviations: MP, myenteric plexus; PGP9.5, protein gene product 9.5; WAS, water avoidance stress.
    Cht1, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Horizon Discovery cht1
    Effect of CTL knockdown on choline transport and ACh secretion. CTL subtypes 1–5 and <t>CHT1</t> were knocked-down by RNAi and cells processed as described in the methods. A. Isoform specific siRNAs decreased levels of CTL1, 2, 4 and 5 mRNA respectively by 50–70% compared to control siRNA-treated cells without affecting levels of other CTL’s. Knockdown for CHT1 and CTL3 are not shown since they were below the level of detection in H82 cells and were included as negative controls for effects on ACh secretion. Data are mean ± SE of at least four replicates in two separate experiments. *P
    Cht1, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Abcam anti cht1
    Effect of CTL knockdown on choline transport and ACh secretion. CTL subtypes 1–5 and <t>CHT1</t> were knocked-down by RNAi and cells processed as described in the methods. A. Isoform specific siRNAs decreased levels of CTL1, 2, 4 and 5 mRNA respectively by 50–70% compared to control siRNA-treated cells without affecting levels of other CTL’s. Knockdown for CHT1 and CTL3 are not shown since they were below the level of detection in H82 cells and were included as negative controls for effects on ACh secretion. Data are mean ± SE of at least four replicates in two separate experiments. *P
    Anti Cht1, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cht 1  (Abcam)
    90
    Abcam cht 1
    Effect of CTL knockdown on choline transport and ACh secretion. CTL subtypes 1–5 and <t>CHT1</t> were knocked-down by RNAi and cells processed as described in the methods. A. Isoform specific siRNAs decreased levels of CTL1, 2, 4 and 5 mRNA respectively by 50–70% compared to control siRNA-treated cells without affecting levels of other CTL’s. Knockdown for CHT1 and CTL3 are not shown since they were below the level of detection in H82 cells and were included as negative controls for effects on ACh secretion. Data are mean ± SE of at least four replicates in two separate experiments. *P
    Cht 1, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology cht1
    Protein and mRNA levels of high-affinity choline transporter 1 <t>(CHT1)</t> in the colon. (A) Western blot analysis revealing that CHT1 protein expression in water avoidance stress (WAS) rats is increased compared to that in control rats. (B) Quantitative real-time polymerase chain reaction analysis showing that the CHT1 mRNA levels of WAS rats are higher than those of control rats. * P
    Cht1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Novus Biologicals cht1 antibody
    (A) Representative immunoblots showing Chk-α, <t>CHT1,</t> and CTL1 proteins in BxPC3, Panc1, Pa02C, and Pa04C tumors, and in tissue obtained from normal mouse pancreas. GAPDH was used as loading control (n = 3). The arrow marks CTL1. (B) Representative
    Cht1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad anti cht1 fitc
    (A) Representative immunoblots showing Chk-α, <t>CHT1,</t> and CTL1 proteins in BxPC3, Panc1, Pa02C, and Pa04C tumors, and in tissue obtained from normal mouse pancreas. GAPDH was used as loading control (n = 3). The arrow marks CTL1. (B) Representative
    Anti Cht1 Fitc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad cht 1 hydroxyapatite column
    (A) Representative immunoblots showing Chk-α, <t>CHT1,</t> and CTL1 proteins in BxPC3, Panc1, Pa02C, and Pa04C tumors, and in tissue obtained from normal mouse pancreas. GAPDH was used as loading control (n = 3). The arrow marks CTL1. (B) Representative
    Cht 1 Hydroxyapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore anti cht1
    Both of the nuclear translocation and catalytic activity of ChAT are required for <t>CHT1</t> induction by ChAT. A , hChAT mRNA level in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. Total RNA was extracted from the cultured cells and used as a template for a real time PCR assay. Values are the mean ± S.E. The non-parametric Mann-Whitney test was used for statistical analysis. ns represents no significant difference. B , real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. *, p
    Anti Cht1, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad cht1 buffer
    Both of the nuclear translocation and catalytic activity of ChAT are required for <t>CHT1</t> induction by ChAT. A , hChAT mRNA level in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. Total RNA was extracted from the cultured cells and used as a template for a real time PCR assay. Values are the mean ± S.E. The non-parametric Mann-Whitney test was used for statistical analysis. ns represents no significant difference. B , real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. *, p
    Cht1 Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology anti cht1 monoclonal antibody
    Western blotting and quantitative RT-PCR analysis of <t>CHT1</t> expression in dorsal root ganglion (DRG). (a) Representative Western blotting for CHT1 in colonic DRGs. (b) Levels of CHT1 protein measured by Western blotting analysis. P
    Anti Cht1 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology mouse anti rat polyclonal cht1 antibody
    Western blotting and quantitative RT-PCR analysis of <t>CHT1</t> expression in dorsal root ganglion (DRG). (a) Representative Western blotting for CHT1 in colonic DRGs. (b) Levels of CHT1 protein measured by Western blotting analysis. P
    Mouse Anti Rat Polyclonal Cht1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Antagene rabbit anti cht1 polyclonal antibody
    Western blotting and quantitative RT-PCR analysis of <t>CHT1</t> expression in dorsal root ganglion (DRG). (a) Representative Western blotting for CHT1 in colonic DRGs. (b) Levels of CHT1 protein measured by Western blotting analysis. P
    Rabbit Anti Cht1 Polyclonal Antibody, supplied by Antagene, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distribution of CHT1 in the MP of colon. Notes: Immunofluorescence for CHT1, PGP9.5, and merged picture on whole-mount preparations of colonic MP from control ( A ), WAS ( B ), and WAS + MKC-231 rats ( C ) (200×) was displayed. The percentage of CHT1-positive neurons in the MP of WAS is significantly higher than that in the control group. In addition, the percentage of CHT1-positive neurons in the WAS + MKC-231 group is enhanced compared to that in the WAS group. Bars = 100 µm; n of each group = 6. Abbreviations: MP, myenteric plexus; PGP9.5, protein gene product 9.5; WAS, water avoidance stress.

    Journal: Journal of Pain Research

    Article Title: Upregulation of the high-affinity choline transporter in colon relieves stress-induced hyperalgesia

    doi: 10.2147/JPR.S164186

    Figure Lengend Snippet: Distribution of CHT1 in the MP of colon. Notes: Immunofluorescence for CHT1, PGP9.5, and merged picture on whole-mount preparations of colonic MP from control ( A ), WAS ( B ), and WAS + MKC-231 rats ( C ) (200×) was displayed. The percentage of CHT1-positive neurons in the MP of WAS is significantly higher than that in the control group. In addition, the percentage of CHT1-positive neurons in the WAS + MKC-231 group is enhanced compared to that in the WAS group. Bars = 100 µm; n of each group = 6. Abbreviations: MP, myenteric plexus; PGP9.5, protein gene product 9.5; WAS, water avoidance stress.

    Article Snippet: MKC-231, a choline uptake enhancer, was administered to explore the role of CHT1 in stress-induced hyperalgesia.

    Techniques: Immunofluorescence

    Immunostaining of CHT1 in the colon. Notes: ( A ) Immunohistochemical staining for CHT1 reveals that the AOD of CHT1 in the muscular layer of WAS rats (right) is increased compared to that of control rats (left) (200×). ( B ) Immunofluorescence staining for CHT1 shows that the density of CHT1 in the muscular layer is significantly elevated after 10 days of WAS (200×). Both the black and white arrows indicate CHT1-positive cells that are mainly fusiform in shape and found within the circular muscle, exhibiting the morphologic characteristics of ICC and smooth muscle cells. Both black and white arrowheads indicate CHT1-positive cells that are predominantly round in shape, located between the circular muscle and longitudinal muscle, showing the hallmark of MP neurons. Bars: (A) = 100 µm, (B) = 100 µm; n of each group = 6. Abbreviations: AOD, average optical density; ICC, interstitial cells of Cajal; MP, myenteric plexus; WAS, water avoidance stress.

    Journal: Journal of Pain Research

    Article Title: Upregulation of the high-affinity choline transporter in colon relieves stress-induced hyperalgesia

    doi: 10.2147/JPR.S164186

    Figure Lengend Snippet: Immunostaining of CHT1 in the colon. Notes: ( A ) Immunohistochemical staining for CHT1 reveals that the AOD of CHT1 in the muscular layer of WAS rats (right) is increased compared to that of control rats (left) (200×). ( B ) Immunofluorescence staining for CHT1 shows that the density of CHT1 in the muscular layer is significantly elevated after 10 days of WAS (200×). Both the black and white arrows indicate CHT1-positive cells that are mainly fusiform in shape and found within the circular muscle, exhibiting the morphologic characteristics of ICC and smooth muscle cells. Both black and white arrowheads indicate CHT1-positive cells that are predominantly round in shape, located between the circular muscle and longitudinal muscle, showing the hallmark of MP neurons. Bars: (A) = 100 µm, (B) = 100 µm; n of each group = 6. Abbreviations: AOD, average optical density; ICC, interstitial cells of Cajal; MP, myenteric plexus; WAS, water avoidance stress.

    Article Snippet: MKC-231, a choline uptake enhancer, was administered to explore the role of CHT1 in stress-induced hyperalgesia.

    Techniques: Immunostaining, Immunohistochemistry, Staining, Immunofluorescence, Immunocytochemistry

    Effect of MKC-231 on pain threshold and CHT1 expression in WAS rats. Notes: ( A ) Immunoblots (left) and a histogram (right) for CHT1 in the colon of WAS and WAS-MKC-231-treated rats reveal that the ratios of CHT1 to β-actin expression in WAS rats are considerably increased after 10 consecutive days of MKC-231 treatment. ( B ) The ACh content of WAS rats is higher than that of the control rats, but lower than that of the MKC-231-treated rats. ( C ) The AWR score of WAS + MKC-231 rats is significantly reduced in contrast to the WAS group. ( D ) Withdrawal events for the WAS + MKC-231 group in the VFF test are declined compared to those in the WAS group. ( E ) VMR amplitude of the WAS + MKC-231 group is lower than that of the WAS rats. n of each group = 6; * P

    Journal: Journal of Pain Research

    Article Title: Upregulation of the high-affinity choline transporter in colon relieves stress-induced hyperalgesia

    doi: 10.2147/JPR.S164186

    Figure Lengend Snippet: Effect of MKC-231 on pain threshold and CHT1 expression in WAS rats. Notes: ( A ) Immunoblots (left) and a histogram (right) for CHT1 in the colon of WAS and WAS-MKC-231-treated rats reveal that the ratios of CHT1 to β-actin expression in WAS rats are considerably increased after 10 consecutive days of MKC-231 treatment. ( B ) The ACh content of WAS rats is higher than that of the control rats, but lower than that of the MKC-231-treated rats. ( C ) The AWR score of WAS + MKC-231 rats is significantly reduced in contrast to the WAS group. ( D ) Withdrawal events for the WAS + MKC-231 group in the VFF test are declined compared to those in the WAS group. ( E ) VMR amplitude of the WAS + MKC-231 group is lower than that of the WAS rats. n of each group = 6; * P

    Article Snippet: MKC-231, a choline uptake enhancer, was administered to explore the role of CHT1 in stress-induced hyperalgesia.

    Techniques: Expressing, Western Blot

    Protein and mRNA levels of CHT1 in the colon. Notes: ( A ) Western blot analysis reveals that CHT1 protein expression is enhanced in WAS rats compared to control rats, whereas the elevated CHT1 expression is partly abolished after treatment with PDTC. ( B ) CHT1 mRNA levels in WAS-exposed rats are higher than those in control rats, and are considerably declined after treatment with PDTC. n of each group =6; * P

    Journal: Journal of Pain Research

    Article Title: Upregulation of the high-affinity choline transporter in colon relieves stress-induced hyperalgesia

    doi: 10.2147/JPR.S164186

    Figure Lengend Snippet: Protein and mRNA levels of CHT1 in the colon. Notes: ( A ) Western blot analysis reveals that CHT1 protein expression is enhanced in WAS rats compared to control rats, whereas the elevated CHT1 expression is partly abolished after treatment with PDTC. ( B ) CHT1 mRNA levels in WAS-exposed rats are higher than those in control rats, and are considerably declined after treatment with PDTC. n of each group =6; * P

    Article Snippet: MKC-231, a choline uptake enhancer, was administered to explore the role of CHT1 in stress-induced hyperalgesia.

    Techniques: Western Blot, Expressing

    Effect of CTL knockdown on choline transport and ACh secretion. CTL subtypes 1–5 and CHT1 were knocked-down by RNAi and cells processed as described in the methods. A. Isoform specific siRNAs decreased levels of CTL1, 2, 4 and 5 mRNA respectively by 50–70% compared to control siRNA-treated cells without affecting levels of other CTL’s. Knockdown for CHT1 and CTL3 are not shown since they were below the level of detection in H82 cells and were included as negative controls for effects on ACh secretion. Data are mean ± SE of at least four replicates in two separate experiments. *P

    Journal: Journal of neurochemistry

    Article Title: Choline transporter-like protein 4 (CTL4) links to non-neuronal acetylcholine synthesis

    doi: 10.1111/jnc.12298

    Figure Lengend Snippet: Effect of CTL knockdown on choline transport and ACh secretion. CTL subtypes 1–5 and CHT1 were knocked-down by RNAi and cells processed as described in the methods. A. Isoform specific siRNAs decreased levels of CTL1, 2, 4 and 5 mRNA respectively by 50–70% compared to control siRNA-treated cells without affecting levels of other CTL’s. Knockdown for CHT1 and CTL3 are not shown since they were below the level of detection in H82 cells and were included as negative controls for effects on ACh secretion. Data are mean ± SE of at least four replicates in two separate experiments. *P

    Article Snippet: On-Target Plus smart pool siRNAs against CTL1, 2, 3, 5 and CHT1 were purchased from Dharmacon (Chicago, IL, USA).

    Techniques: CTL Assay

    Protein and mRNA levels of high-affinity choline transporter 1 (CHT1) in the colon. (A) Western blot analysis revealing that CHT1 protein expression in water avoidance stress (WAS) rats is increased compared to that in control rats. (B) Quantitative real-time polymerase chain reaction analysis showing that the CHT1 mRNA levels of WAS rats are higher than those of control rats. * P

    Journal: Journal of Neurogastroenterology and Motility

    Article Title: Role of High-affinity Choline Transporter 1 in Colonic Hypermotility in a Rat Model of Irritable Bowel Syndrome

    doi: 10.5056/jnm18040

    Figure Lengend Snippet: Protein and mRNA levels of high-affinity choline transporter 1 (CHT1) in the colon. (A) Western blot analysis revealing that CHT1 protein expression in water avoidance stress (WAS) rats is increased compared to that in control rats. (B) Quantitative real-time polymerase chain reaction analysis showing that the CHT1 mRNA levels of WAS rats are higher than those of control rats. * P

    Article Snippet: The blots were then incubated overnight at 4°C with the primary antibody against CHT1 (Santa Cruz Biotechnology, Dallas, TX, USA) or β-actin (Beyotime).

    Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction

    Immunostaining of high-affinity choline transporter 1 (CHT1) in the colon. (A) Double-immunostaining for CHT1 and transmembrane member 16A (TMEM16A) in longitudinal colonic sections from control and water avoidance stress (WAS) rats (×400) revealing that CHT1 in the circular muscle of both naive and WAS rats is exclusively expressed in TMEM16A-immunoreactive (IR) cells and the number of CHT1-IR cells in the muscular layer of WAS rats is significantly increased compared to that of control rats. (B) Double-immunofluorescence on whole-mount preparations of colonic myenteric plexus (MP) (×400) illustrating that TMEM16A is robustly expressed in colonic MP and that all the CHT1-IR cells are co-labelled with TMEM16A. The percentage of CHT1-IR cells in the MP is substantially elevated after 10 days of WAS. Different layers were marked out. White arrows indicate CHT1-positive cells that are mainly fusiform in shape and found within circular muscle, exhibiting the morphological characteristics of interstitial cells of Cajal. White arrowheads indicate CHT1-positive cells that are predominantly round in shape and located between circular and longitudinal muscles, showing the hallmark of MP neurons. ML, mucosal layer; SML, submucosal layer; CML, circular muscle layer; LML, longitudinal muscle layer.

    Journal: Journal of Neurogastroenterology and Motility

    Article Title: Role of High-affinity Choline Transporter 1 in Colonic Hypermotility in a Rat Model of Irritable Bowel Syndrome

    doi: 10.5056/jnm18040

    Figure Lengend Snippet: Immunostaining of high-affinity choline transporter 1 (CHT1) in the colon. (A) Double-immunostaining for CHT1 and transmembrane member 16A (TMEM16A) in longitudinal colonic sections from control and water avoidance stress (WAS) rats (×400) revealing that CHT1 in the circular muscle of both naive and WAS rats is exclusively expressed in TMEM16A-immunoreactive (IR) cells and the number of CHT1-IR cells in the muscular layer of WAS rats is significantly increased compared to that of control rats. (B) Double-immunofluorescence on whole-mount preparations of colonic myenteric plexus (MP) (×400) illustrating that TMEM16A is robustly expressed in colonic MP and that all the CHT1-IR cells are co-labelled with TMEM16A. The percentage of CHT1-IR cells in the MP is substantially elevated after 10 days of WAS. Different layers were marked out. White arrows indicate CHT1-positive cells that are mainly fusiform in shape and found within circular muscle, exhibiting the morphological characteristics of interstitial cells of Cajal. White arrowheads indicate CHT1-positive cells that are predominantly round in shape and located between circular and longitudinal muscles, showing the hallmark of MP neurons. ML, mucosal layer; SML, submucosal layer; CML, circular muscle layer; LML, longitudinal muscle layer.

    Article Snippet: The blots were then incubated overnight at 4°C with the primary antibody against CHT1 (Santa Cruz Biotechnology, Dallas, TX, USA) or β-actin (Beyotime).

    Techniques: Immunostaining, Double Immunostaining, Immunofluorescence

    Influence of MKC-231, a choline uptake enhancer, on high-affinity choline transporter 1 (CHT1) expression and intestinal motility in vivo. (A) Immunoblots (left) and histogram (right) for CHT1 in the colon from control and MKC-231-treated rats showing that the ratios of CHT1 to β-actin expression in control rats are significantly increased after 10 consecutive days of MKC-231 treatment. (B) Merged pictures of immunostaining for CHT1, transmembrane member 16A (TMEM16A), and DAPI (4′,6-diamidino-2-phenylindole) in longitudinal colonic sections from control (left) and MKC-231 rats (right) (×400) revealing that the density of CHT1 labelling in the colonic muscular layer is considerably elevated after treatment with MKC-231. White arrows indicate CHT1-positive intramuscular interstitial cells of Cajal. White arrowheads indicate CHT1-immunoreactive (IR) neurons in the myenteric plexus. (C) The intestinal transit time of MKC-231-rats is reduced compared to that of control rats. (D) The fecal water content of the MKC-231 group is considerably increased compared with that of the control group. * P

    Journal: Journal of Neurogastroenterology and Motility

    Article Title: Role of High-affinity Choline Transporter 1 in Colonic Hypermotility in a Rat Model of Irritable Bowel Syndrome

    doi: 10.5056/jnm18040

    Figure Lengend Snippet: Influence of MKC-231, a choline uptake enhancer, on high-affinity choline transporter 1 (CHT1) expression and intestinal motility in vivo. (A) Immunoblots (left) and histogram (right) for CHT1 in the colon from control and MKC-231-treated rats showing that the ratios of CHT1 to β-actin expression in control rats are significantly increased after 10 consecutive days of MKC-231 treatment. (B) Merged pictures of immunostaining for CHT1, transmembrane member 16A (TMEM16A), and DAPI (4′,6-diamidino-2-phenylindole) in longitudinal colonic sections from control (left) and MKC-231 rats (right) (×400) revealing that the density of CHT1 labelling in the colonic muscular layer is considerably elevated after treatment with MKC-231. White arrows indicate CHT1-positive intramuscular interstitial cells of Cajal. White arrowheads indicate CHT1-immunoreactive (IR) neurons in the myenteric plexus. (C) The intestinal transit time of MKC-231-rats is reduced compared to that of control rats. (D) The fecal water content of the MKC-231 group is considerably increased compared with that of the control group. * P

    Article Snippet: The blots were then incubated overnight at 4°C with the primary antibody against CHT1 (Santa Cruz Biotechnology, Dallas, TX, USA) or β-actin (Beyotime).

    Techniques: Expressing, In Vivo, Western Blot, Immunostaining

    Influence of ammonium pyrrolidine dithiocarbamate (PDTC) on high-affinity choline transporter 1 (CHT1) expression and intestinal motility. (A) Immunoblots (left) and histogram (right) for CHT1 in the colon from water avoidance stress (WAS) and PDTC-treated rats showing that the obtained CHT1 and β-actin level ratios of the PDTC group are significantly decreased compared to that in WAS rats. (B) The intestinal transit time of WAS rats are increased after treatment with PDTC. (C) The fecal water content of PDTC group is decreased compared with the WAS rats. * P

    Journal: Journal of Neurogastroenterology and Motility

    Article Title: Role of High-affinity Choline Transporter 1 in Colonic Hypermotility in a Rat Model of Irritable Bowel Syndrome

    doi: 10.5056/jnm18040

    Figure Lengend Snippet: Influence of ammonium pyrrolidine dithiocarbamate (PDTC) on high-affinity choline transporter 1 (CHT1) expression and intestinal motility. (A) Immunoblots (left) and histogram (right) for CHT1 in the colon from water avoidance stress (WAS) and PDTC-treated rats showing that the obtained CHT1 and β-actin level ratios of the PDTC group are significantly decreased compared to that in WAS rats. (B) The intestinal transit time of WAS rats are increased after treatment with PDTC. (C) The fecal water content of PDTC group is decreased compared with the WAS rats. * P

    Article Snippet: The blots were then incubated overnight at 4°C with the primary antibody against CHT1 (Santa Cruz Biotechnology, Dallas, TX, USA) or β-actin (Beyotime).

    Techniques: Expressing, Western Blot

    (A) Representative immunoblots showing Chk-α, CHT1, and CTL1 proteins in BxPC3, Panc1, Pa02C, and Pa04C tumors, and in tissue obtained from normal mouse pancreas. GAPDH was used as loading control (n = 3). The arrow marks CTL1. (B) Representative

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Metabolic Imaging of Pancreatic Ductal Adenocarcinoma Detects Altered Choline Metabolism

    doi: 10.1158/1078-0432.CCR-14-0964

    Figure Lengend Snippet: (A) Representative immunoblots showing Chk-α, CHT1, and CTL1 proteins in BxPC3, Panc1, Pa02C, and Pa04C tumors, and in tissue obtained from normal mouse pancreas. GAPDH was used as loading control (n = 3). The arrow marks CTL1. (B) Representative

    Article Snippet: Approximately 60 – 75 µg of total protein were resolved on 10% SDS-PAGE, transferred onto nitrocellulose membranes, and probed for 2 h at room temperature with an in-house human specific rabbit polyclonal Chk-α antibody (dilution 1:100) , a mouse and human cross reactive rabbit polyclonal CHT1 antibody (dilution 1:2000) (Cat. No. NBP1-62339) Novus Biologicals, Littleton, CO), or a human and mouse cross reactive polyclonal CTL1 antibody (1:1000) (Cat. No. TA315247, OriGene).

    Techniques: Western Blot

    (A) Representative immunoblots from a panel of pancreatic cell lines showing Chk-α, CHT1, and CTL1 expression with GAPDH as loading control. (B) Representative high-resolution 1 H spectra of the choline-containing compounds region obtained from

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Metabolic Imaging of Pancreatic Ductal Adenocarcinoma Detects Altered Choline Metabolism

    doi: 10.1158/1078-0432.CCR-14-0964

    Figure Lengend Snippet: (A) Representative immunoblots from a panel of pancreatic cell lines showing Chk-α, CHT1, and CTL1 expression with GAPDH as loading control. (B) Representative high-resolution 1 H spectra of the choline-containing compounds region obtained from

    Article Snippet: Approximately 60 – 75 µg of total protein were resolved on 10% SDS-PAGE, transferred onto nitrocellulose membranes, and probed for 2 h at room temperature with an in-house human specific rabbit polyclonal Chk-α antibody (dilution 1:100) , a mouse and human cross reactive rabbit polyclonal CHT1 antibody (dilution 1:2000) (Cat. No. NBP1-62339) Novus Biologicals, Littleton, CO), or a human and mouse cross reactive polyclonal CTL1 antibody (1:1000) (Cat. No. TA315247, OriGene).

    Techniques: Western Blot, Expressing

    Both of the nuclear translocation and catalytic activity of ChAT are required for CHT1 induction by ChAT. A , hChAT mRNA level in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. Total RNA was extracted from the cultured cells and used as a template for a real time PCR assay. Values are the mean ± S.E. The non-parametric Mann-Whitney test was used for statistical analysis. ns represents no significant difference. B , real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Nuclear Choline Acetyltransferase Activates Transcription of a High-affinity Choline Transporter *

    doi: 10.1074/jbc.M110.147611

    Figure Lengend Snippet: Both of the nuclear translocation and catalytic activity of ChAT are required for CHT1 induction by ChAT. A , hChAT mRNA level in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. Total RNA was extracted from the cultured cells and used as a template for a real time PCR assay. Values are the mean ± S.E. The non-parametric Mann-Whitney test was used for statistical analysis. ns represents no significant difference. B , real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP ( wt-hChAT ), NLS2-(−)-hChAT-GFP ( NLS2 -(−)), inactive hChAT-GFP ( Inactive ), GFP alone, and nontransfected SH-SY5Y cells. *, p

    Article Snippet: The primary antibodies used were anti-GFP (mouse monoclonal, Roche, diluted 1:2500), anti-β-actin (mouse monoclonal, MAB1501, Chemicon International Inc., Billerica, MA, diluted 1:2000), anti-GAPDH (mouse monoclonal, MAB374, Chemicon, diluted 1:2000), anti-ChAT (rabbit polyclonal generated in our laboratory , diluted 1:5000), and anti-CHT1 (rabbit polyclonal, AB5966, Chemicon, diluted 1:1000).

    Techniques: Translocation Assay, Activity Assay, Cell Culture, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Quantitative RT-PCR, Expressing

    A muscarinic antagonist, atropine, did not reverse CHT1 induction by ChAT. Real time RT-PCR analysis of CHT1 in wt-hChAT-GFP cells treated with various concentrations of atropine for 72 h. Total RNA was then extracted from the cells and the relative level of CHT1 mRNA was evaluated by a real time PCR assay. Values are the mean ± S.E. No significant difference was detected by using a post hoc Tukey-Kramer test.

    Journal: The Journal of Biological Chemistry

    Article Title: Nuclear Choline Acetyltransferase Activates Transcription of a High-affinity Choline Transporter *

    doi: 10.1074/jbc.M110.147611

    Figure Lengend Snippet: A muscarinic antagonist, atropine, did not reverse CHT1 induction by ChAT. Real time RT-PCR analysis of CHT1 in wt-hChAT-GFP cells treated with various concentrations of atropine for 72 h. Total RNA was then extracted from the cells and the relative level of CHT1 mRNA was evaluated by a real time PCR assay. Values are the mean ± S.E. No significant difference was detected by using a post hoc Tukey-Kramer test.

    Article Snippet: The primary antibodies used were anti-GFP (mouse monoclonal, Roche, diluted 1:2500), anti-β-actin (mouse monoclonal, MAB1501, Chemicon International Inc., Billerica, MA, diluted 1:2000), anti-GAPDH (mouse monoclonal, MAB374, Chemicon, diluted 1:2000), anti-ChAT (rabbit polyclonal generated in our laboratory , diluted 1:5000), and anti-CHT1 (rabbit polyclonal, AB5966, Chemicon, diluted 1:1000).

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP-M and NLS2-(−)-hChAT-GFP cells. Total RNA was extracted from wt-hChAT-GFP ( wt-hChAT ), wt-hChAT-GFP-M ( wt-hChAT-M ), NLS2-(−)-hChAT-GFP (( NLS2 -(−)), and GFP alone cells and was used as a template for a real time PCR assay. Values are the mean ± S.E. * indicates p

    Journal: The Journal of Biological Chemistry

    Article Title: Nuclear Choline Acetyltransferase Activates Transcription of a High-affinity Choline Transporter *

    doi: 10.1074/jbc.M110.147611

    Figure Lengend Snippet: Real time RT-PCR analysis of CHT1 mRNA expression in wt-hChAT-GFP-M and NLS2-(−)-hChAT-GFP cells. Total RNA was extracted from wt-hChAT-GFP ( wt-hChAT ), wt-hChAT-GFP-M ( wt-hChAT-M ), NLS2-(−)-hChAT-GFP (( NLS2 -(−)), and GFP alone cells and was used as a template for a real time PCR assay. Values are the mean ± S.E. * indicates p

    Article Snippet: The primary antibodies used were anti-GFP (mouse monoclonal, Roche, diluted 1:2500), anti-β-actin (mouse monoclonal, MAB1501, Chemicon International Inc., Billerica, MA, diluted 1:2000), anti-GAPDH (mouse monoclonal, MAB374, Chemicon, diluted 1:2000), anti-ChAT (rabbit polyclonal generated in our laboratory , diluted 1:5000), and anti-CHT1 (rabbit polyclonal, AB5966, Chemicon, diluted 1:1000).

    Techniques: Quantitative RT-PCR, Expressing, Real-time Polymerase Chain Reaction

    Western blotting and quantitative RT-PCR analysis of CHT1 expression in dorsal root ganglion (DRG). (a) Representative Western blotting for CHT1 in colonic DRGs. (b) Levels of CHT1 protein measured by Western blotting analysis. P

    Journal: Gastroenterology Research and Practice

    Article Title: Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

    doi: 10.1155/2018/9252984

    Figure Lengend Snippet: Western blotting and quantitative RT-PCR analysis of CHT1 expression in dorsal root ganglion (DRG). (a) Representative Western blotting for CHT1 in colonic DRGs. (b) Levels of CHT1 protein measured by Western blotting analysis. P

    Article Snippet: After blocking with 2% BSA and washing, membranes were incubated with anti-CHT1 monoclonal antibody (mouse anti-rat IgG, 1 : 200, Santa Cruz, CA, USA) at 4°C overnight.

    Techniques: Western Blot, Quantitative RT-PCR, Expressing

    Immunohistochemistry analysis of CHT1 expression in dorsal root ganglion (DRG). Immunohistochemistry revealed an enhancement of CHT1 staining in the water avoidance stress group (a, b) compared to the sham stress group (c, d). Scale bar = 50 mm.

    Journal: Gastroenterology Research and Practice

    Article Title: Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

    doi: 10.1155/2018/9252984

    Figure Lengend Snippet: Immunohistochemistry analysis of CHT1 expression in dorsal root ganglion (DRG). Immunohistochemistry revealed an enhancement of CHT1 staining in the water avoidance stress group (a, b) compared to the sham stress group (c, d). Scale bar = 50 mm.

    Article Snippet: After blocking with 2% BSA and washing, membranes were incubated with anti-CHT1 monoclonal antibody (mouse anti-rat IgG, 1 : 200, Santa Cruz, CA, USA) at 4°C overnight.

    Techniques: Immunohistochemistry, Expressing, Staining