Journal: Cells

Article Title: Heat-Treated Lacticaseibacillus rhamnosus Strains Modulate Inflammatory and Metabolic Processes in In Vitro Systems Relevant to Canine Osteoarthritis

doi: 10.3390/cells15040336

Figure Lengend Snippet: Screening of the anti-inflammatory effect of different heat-treated strains in canine chondrocytes. IL-6 secretion by primary canine chondrocytes stimulated with IL-1β was measured in the presence of a collection of candidates. Data are represented as the percentage of IL-6 secretion relative to IL-1β stimulated condition without inactivated cells. Control condition refers to chondrocytes stimulated with IL-1β, whereas negative control (NC) corresponds to chondrocytes without stimulation. Data are presented as the mean ± SD of three independent experiments. Statistical test: One-way ANOVA with Dunnett’s multiple comparisons test (Postbiotic vs. Control). Significance levels were indicated as follows: p -value > 0.05 (ns: non-significant), p -value < 0.01(**), p -value < 0.0001 (****).

Article Snippet: Alginate beads were washed 5 times with NaCl solution (0.9%) and finally, one more time in chondrocyte differentiation medium (Cell Applications Inc., San Diego, CA, USA).

Techniques: Control, Negative Control

Journal: Cells

Article Title: Heat-Treated Lacticaseibacillus rhamnosus Strains Modulate Inflammatory and Metabolic Processes in In Vitro Systems Relevant to Canine Osteoarthritis

doi: 10.3390/cells15040336

Figure Lengend Snippet: Insoluble type II collagen production by primary canine chondrocytes cultured in spheroids structure. Data are presented as the mean ± SD of three independent experiments and expressed as the percentage of insoluble type II collagen production relative to the control condition. In the control condition, canine chondrocytes were cultured without supplementation of heat-treated strains. Statistical test: Kruskal-Wallis test, followed by Dunn’s multiple comparison test (Control vs. Postbiotic). Significance levels were indicated as follows: p -value < 0.05 (*).

Article Snippet: Alginate beads were washed 5 times with NaCl solution (0.9%) and finally, one more time in chondrocyte differentiation medium (Cell Applications Inc., San Diego, CA, USA).

Techniques: Cell Culture, Control, Comparison

Journal: Open Veterinary Journal

Article Title: Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease

doi: 10.5455/OVJ.2024.v14.i5.12

Figure Lengend Snippet: Trilineage differentiation potency. Canine LCPD-affected femoral head-derived adherent cells (A-C) and BMMSCs (D-F) differentiated into adipocytes (A and D), osteoblasts (B and E), and chondrocytes (C and F). (A and D): Sudan III staining, (B and E): Alizarin Red staining, and (C and F): Alcian blue staining. Scale bars = 100 μm or 1 mm.

Article Snippet: To induce differentiation into chondrocytes, 5 × 10 5 cells were placed in 15 ml polypropylene tubes, centrifuged to form pellets, and cultured with canine chondrocyte differentiation medium (Cell Applications, Inc.) for 3 weeks.

Techniques: Derivative Assay, Staining

Journal: BMC Musculoskeletal Disorders

Article Title: ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model

doi: 10.1186/1471-2474-11-221

Figure Lengend Snippet: ARG098 cytotoxicity to human chondrocytes . (A) ARG098 binding activity to human chondrocytes was assessed by flow cytometory. The white area is stained with human IgM and the black area is stained with ARG098. (B) RA synoviocytes pretreated with anti-Fas neutralizing antibody clone SM1/23. (C) Representative cell viability of human chondrocytes following ARG098 treatment (Experiment 1: from 0.1 to 1000 ng/mL, Experiment 2: from 1 to 100 μg/mL) versus untreated condition, as measured by the WST assay. Black circles and black triangles, ARG098; white circles and white triangles, human IgM. Each point represents mean ± SEM (n = 5).

Article Snippet: The sources of the other materials used in this study are as follows: human IgM was obtained from ICN Biomedicals Inc. (Aliso Viejo, CA, USA), chondrocyte basal medium supplemented with chondrocyte growth supplement was obtained from Cell Applications, Inc. (San Diego, CA, USA), and the neutralizing antibody anti-human APO-1/Fas (SM1/23) was obtained from Bender Medsystems GmbH (Vienna, Austria).

Techniques: Binding Assay, Activity Assay, Staining, WST Assay