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Image Search Results
Journal: Aging (Albany NY)
Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis
doi: 10.18632/aging.205334
Figure Lengend Snippet: The expression and function of SLC27A5 in HCC. ( A – C ) The transcriptional expression of SLC27A5 in HCC and normal tissues of TCGA-LIHC ( A ), GSE14520 ( B ) and GSE64041 ( C ) datasets. ( D ) The protein level of SLC27A5 in HCC and normal tissues. ( E , F ) CCK-8 assay for HepG2 ( E ) and LM-3 ( F ) cells overexpressing SL27A5. ( G , H ) HepG2 ( G ) and LM-3 ( H ) cells were subject to flow cytometry analysis for cell cycle. ( I ) HCC cells overexpressing SLC27A5 were subject to EdU staining. ( J ) HCC cells overexpressing SLC27A5 were subject to wound healing assay.
Article Snippet: Western blotting was performed using antibodies against FDX1 (M05441, Boster),
Techniques: Expressing, CCK-8 Assay, Flow Cytometry, Staining, Wound Healing Assay
Journal: Aging (Albany NY)
Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis
doi: 10.18632/aging.205334
Figure Lengend Snippet: SLC27A5 upregulates FDX1 in HCC. ( A , B ) The correlation between the expression of SLC27A5 and that of FDX1 in TCGA-LIHC ( A ) and GSE14520 ( B ) datasets. ( C ) Dot plot showing the dependency scores for SLC27A5 and FDX1 across all tumor cell lines in the Project Achilles/Cancer Dependency Map Portal (DepMap). ( D , E ) The expression of and correlation between SLC27A5 and FDX1 in collected HCC tissue chip. ( F , G ) HepG2 ( F ) and LM-3 ( G ) cells overexpressing SLC27A5 were subject to qRT-PCR analysis. ( H , I ) Western blotting using lysates of HepG2 ( H ) and LM-3 ( I ) cells after overexpressing SLC27A5.
Article Snippet: Western blotting was performed using antibodies against FDX1 (M05441, Boster),
Techniques: Expressing, Quantitative RT-PCR, Western Blot