Journal: International Journal of Oral Science

Article Title: β-Arrestin 1 (ARRB1) serves as a molecular marker of the peripheral circadian rhythm

doi: 10.1038/s41368-019-0065-y

Figure Lengend Snippet: Selection of candidate circadian markers. a Flow chart of the selection process. b Evaluation of the ChIP-on-chip results for the NR1D1 region. Transfected HSG cells tagged with BMAL1 and REV-ERBα were harvested at 12 and 24 h after dexamethasone stimulation, and then ChIP was performed using anti-tagged antibodies. Mock-transfected cells without dexamethasone stimulation were processed in the same manner and are indicated as “–”. Precipitated DNA was analyzed using a NimbleGen Human ChIP–chip array, and the NR1D1 locus and the tiling region are indicated on the map. The red and yellow boxes in the map indicate regions that showed false discovery rates (FDR) of ≤ 0.05 and 0.1 < FDR ≤ 0.2, respectively. c . Venn diagram of the gene set intersect between BMAL1-bound cells at 12 h and REV-ERBα-bound cells at 24 h after dexamethasone stimulation in HSG cells. d Total RNA was prepared from HSG cells harvested at 12 h and 24 h after dexamethasone simulation, and expression was compared using an Agilent microarray. We found 1 394 and 1 197 genes that were up- and downregulated, respectively, at 12 h compared with their expression 24 h after stimulation. e Overlap between genes that might be controlled by the Clock system and genes that were significantly upregulated at 12 h compared to 24 h after stimulation

Article Snippet: The amplified DNA was sent to NimbleGen Systems Inc. (Madison, WI, USA) for labeling and array hybridization on a NimbleGen Human ChIP–chip 3 x 720K RefSeq Promoter Array.

Techniques: Selection, Transfection, ChIP-chip, Expressing, Microarray

Journal: Molecular Vision

Article Title: The expression of cytokines in aqueous humor of high myopic patients with cataracts

doi:

Figure Lengend Snippet: The expression levels of cytokines marked changes in high myopic patients with cataracts and non-myopic patients with cataracts by Quantibody Custom Array.

Article Snippet: To reduce errors and improve accuracy, a quantitative protein detection chip RayBio Quantibody Custom Array (product QAH-CUST; RayBio; RayBiotech, Norcross, GA) was used for the verification of the cytokines of interest in the confirmatory stage.

Techniques: Expressing

Journal: Molecular Vision

Article Title: The expression of cytokines in aqueous humor of high myopic patients with cataracts

doi:

Figure Lengend Snippet: The differences in the fluorescence of the cytokines with statistical significance in the Quantibody Custom Array. The higher the fluorescent brightness, the higher the concentration of the cytokine in the aqueous humor.

Article Snippet: To reduce errors and improve accuracy, a quantitative protein detection chip RayBio Quantibody Custom Array (product QAH-CUST; RayBio; RayBiotech, Norcross, GA) was used for the verification of the cytokines of interest in the confirmatory stage.

Techniques: Fluorescence, Concentration Assay

Journal: Scientific Reports

Article Title: Serum N -glycan profiling is a potential biomarker for castration-resistant prostate cancer

doi: 10.1038/s41598-019-53384-y

Figure Lengend Snippet: Detection of lectin-reactive glycans on α-1-acid glycoprotein (AGP) using recombinant lectin array chip. The AGP concentration adjusted lectin array analysis showed that terminal α2.3 sialylated glycan ( A ), α2.6 sialylated glycan ( B ), and terminal galactose ( C ), were significantly increased in the CRPC patients. In contrast, branched-LacNAc structure was significantly decreased in the CRPC patients ( D ). RFI: Relative fluorescent intensity.

Article Snippet: Diluted serum was added to the well of recombinant lectin array chip (Rexxam Co. Ltd., Osaka, Japan).

Techniques: Recombinant, Concentration Assay, Glycoproteomics