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  • chikv  (ATCC)
    95
    ATCC chikv
    Chikv, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv - by Bioz Stars, 2021-05
    95/100 stars
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    86
    EUROIMMUN chikungunya virus
    Central nervous system (CNS) imaging abnormalities in patients with evidence of Zika, <t>chikungunya</t> and/or dengue virus infection. A: Encephalomyelitis in a patient with CNS Zika and systemic dengue infection (patient 3). Fluid attenuation inversion recovery [FLAIR] signal abnormality involving the middle cerebellar peduncles, more marked on the right (axial scan). B: Acute disseminated encephalomyelitis in a patient with systemic chikungunya infection (patient 20). Confluent areas of T2 signal abnormality suggesting neuroinflammation consistent with demyelination (coronal scan). C, D, E: Encephalomyelitis with subclinical meningitis in a patient with CNS Zika + chikungunya infection (patient 9). FLAIR signal abnormality involving the medial temporal lobes, amygdala, and a small area of abnormality adjacent to the temporal horn of the left lateral ventricle (C, axial scan). High signal intensity on T2-weighted images in the anterior medulla, and anterior cervical and thoracic cord (D and E, sagittal scans). F, G, H: Myelitis in a patient with CNS Zika + dengue and systemic chikungunya infection (patient 2). Extensive intramedullary signal abnormality of the cervical cord, without evidence of contrast enhancement (F, sagittal T2-weighted scan; G, sagittal T1-weighted scan with gadolinium; H, axial T2-weighted scan). I, J: Facial diplegia with paraesthesia in patient with CNS Zika + chikungunya infection (patient 4). Bilateral facial nerve enhancement on T2-weighted images with gadolinium (axial scan).
    Chikungunya Virus, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikungunya virus/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
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    chikungunya virus - by Bioz Stars, 2021-05
    86/100 stars
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    86
    Illumina Inc chikv
    Identification of dcr-2 null mutant mosquito cell lines. Size distribution and nucleotide analysis of virus-derived small RNAs in u4.4 cells ( A ), dcr-2 FS−1 (C6/36) cells ( B ) and dcr-2 del 33 (C7-10) cells ( C ) infected with <t>CHIKV.</t> Schematics indicating Dcr-2 domains ( D ). The A. <t>albopictus</t> Dcr-2 contains a DExH/D protein family domain (DEAD) and helicase conserved C-terminal domain (H); a domain of unknown function (DUF); a PAZ domain; and tandem RNase III domains. Asterisks indicate locations of deletions in dcr-2 sequences.
    Chikv, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv - by Bioz Stars, 2021-05
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    93
    Abcam eilv chikv
    Comparison of <t>EILV/CHIKV</t> and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.
    Eilv Chikv, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eilv chikv/product/Abcam
    Average 93 stars, based on 1 article reviews
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    eilv chikv - by Bioz Stars, 2021-05
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    N/A
    Size 5mL supernatant Price 25 0 Molecule Name anti chikungunya virus chikv antibodies
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    N/A
    Size 5mL supernatant Price 25 0 Molecule Name anti chikungunya virus chikv antibodies
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    Image Search Results


    Central nervous system (CNS) imaging abnormalities in patients with evidence of Zika, chikungunya and/or dengue virus infection. A: Encephalomyelitis in a patient with CNS Zika and systemic dengue infection (patient 3). Fluid attenuation inversion recovery [FLAIR] signal abnormality involving the middle cerebellar peduncles, more marked on the right (axial scan). B: Acute disseminated encephalomyelitis in a patient with systemic chikungunya infection (patient 20). Confluent areas of T2 signal abnormality suggesting neuroinflammation consistent with demyelination (coronal scan). C, D, E: Encephalomyelitis with subclinical meningitis in a patient with CNS Zika + chikungunya infection (patient 9). FLAIR signal abnormality involving the medial temporal lobes, amygdala, and a small area of abnormality adjacent to the temporal horn of the left lateral ventricle (C, axial scan). High signal intensity on T2-weighted images in the anterior medulla, and anterior cervical and thoracic cord (D and E, sagittal scans). F, G, H: Myelitis in a patient with CNS Zika + dengue and systemic chikungunya infection (patient 2). Extensive intramedullary signal abnormality of the cervical cord, without evidence of contrast enhancement (F, sagittal T2-weighted scan; G, sagittal T1-weighted scan with gadolinium; H, axial T2-weighted scan). I, J: Facial diplegia with paraesthesia in patient with CNS Zika + chikungunya infection (patient 4). Bilateral facial nerve enhancement on T2-weighted images with gadolinium (axial scan).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: The spectrum of neurological disease associated with Zika and chikungunya viruses in adults in Rio de Janeiro, Brazil: A case series

    doi: 10.1371/journal.pntd.0006212

    Figure Lengend Snippet: Central nervous system (CNS) imaging abnormalities in patients with evidence of Zika, chikungunya and/or dengue virus infection. A: Encephalomyelitis in a patient with CNS Zika and systemic dengue infection (patient 3). Fluid attenuation inversion recovery [FLAIR] signal abnormality involving the middle cerebellar peduncles, more marked on the right (axial scan). B: Acute disseminated encephalomyelitis in a patient with systemic chikungunya infection (patient 20). Confluent areas of T2 signal abnormality suggesting neuroinflammation consistent with demyelination (coronal scan). C, D, E: Encephalomyelitis with subclinical meningitis in a patient with CNS Zika + chikungunya infection (patient 9). FLAIR signal abnormality involving the medial temporal lobes, amygdala, and a small area of abnormality adjacent to the temporal horn of the left lateral ventricle (C, axial scan). High signal intensity on T2-weighted images in the anterior medulla, and anterior cervical and thoracic cord (D and E, sagittal scans). F, G, H: Myelitis in a patient with CNS Zika + dengue and systemic chikungunya infection (patient 2). Extensive intramedullary signal abnormality of the cervical cord, without evidence of contrast enhancement (F, sagittal T2-weighted scan; G, sagittal T1-weighted scan with gadolinium; H, axial T2-weighted scan). I, J: Facial diplegia with paraesthesia in patient with CNS Zika + chikungunya infection (patient 4). Bilateral facial nerve enhancement on T2-weighted images with gadolinium (axial scan).

    Article Snippet: [ ] Serum IgM and IgG antibodies to Zika virus NS1 antigen and serum and CSF IgM and IgG antibodies to chikungunya virus were measured using commercial ELISAs (Euroimmun, Luebeck, Germany), according to the manufacturer’s protocol.

    Techniques: Imaging, Infection

    Venn diagram for 22 patients showing virological evidence of CNS or systemic infection with Zika, chikungunya and/or dengue, and clinical presentation with CNS or peripheral nervous system disease. We distinguish virological evidence of CNS or systemic infection (based on PCR/antibody testing) from clinical evidence of CNS or peripheral nervous system disease (based on clinical features). Patients in the inner darker circles have evidence of CNS +/- systemic infection with the respective virus. Those in the outer paler circles have evidence of only systemic infection with the respective virus. Note that patients 1 and 3 had confirmed Zika, +/- dengue; patients 2 and 7 had Zika or dengue or both.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: The spectrum of neurological disease associated with Zika and chikungunya viruses in adults in Rio de Janeiro, Brazil: A case series

    doi: 10.1371/journal.pntd.0006212

    Figure Lengend Snippet: Venn diagram for 22 patients showing virological evidence of CNS or systemic infection with Zika, chikungunya and/or dengue, and clinical presentation with CNS or peripheral nervous system disease. We distinguish virological evidence of CNS or systemic infection (based on PCR/antibody testing) from clinical evidence of CNS or peripheral nervous system disease (based on clinical features). Patients in the inner darker circles have evidence of CNS +/- systemic infection with the respective virus. Those in the outer paler circles have evidence of only systemic infection with the respective virus. Note that patients 1 and 3 had confirmed Zika, +/- dengue; patients 2 and 7 had Zika or dengue or both.

    Article Snippet: [ ] Serum IgM and IgG antibodies to Zika virus NS1 antigen and serum and CSF IgM and IgG antibodies to chikungunya virus were measured using commercial ELISAs (Euroimmun, Luebeck, Germany), according to the manufacturer’s protocol.

    Techniques: Infection, Polymerase Chain Reaction

    Identification of dcr-2 null mutant mosquito cell lines. Size distribution and nucleotide analysis of virus-derived small RNAs in u4.4 cells ( A ), dcr-2 FS−1 (C6/36) cells ( B ) and dcr-2 del 33 (C7-10) cells ( C ) infected with CHIKV. Schematics indicating Dcr-2 domains ( D ). The A. albopictus Dcr-2 contains a DExH/D protein family domain (DEAD) and helicase conserved C-terminal domain (H); a domain of unknown function (DUF); a PAZ domain; and tandem RNase III domains. Asterisks indicate locations of deletions in dcr-2 sequences.

    Journal: PLoS Pathogens

    Article Title: Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma

    doi: 10.1371/journal.ppat.1002470

    Figure Lengend Snippet: Identification of dcr-2 null mutant mosquito cell lines. Size distribution and nucleotide analysis of virus-derived small RNAs in u4.4 cells ( A ), dcr-2 FS−1 (C6/36) cells ( B ) and dcr-2 del 33 (C7-10) cells ( C ) infected with CHIKV. Schematics indicating Dcr-2 domains ( D ). The A. albopictus Dcr-2 contains a DExH/D protein family domain (DEAD) and helicase conserved C-terminal domain (H); a domain of unknown function (DUF); a PAZ domain; and tandem RNase III domains. Asterisks indicate locations of deletions in dcr-2 sequences.

    Article Snippet: Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma To investigate the small RNA populations present in Aedes aegypti and Aedes albopictus mosquitoes infected with CHIKV, we prepared cDNA libraries from the ∼18–35-nt fraction of total RNA and sequenced on an Illumina platform.

    Techniques: Mutagenesis, Derivative Assay, Infection

    B2-mediated suppression of piRNA-like viral small RNAs in dcr-2 null mutant cells. Size distribution and nucleotide analysis of virus-derived small RNAs in dcr-2 FS−1 cells infected with CHIKV-B2 (FHV) ( A ) or CHIKV-B2 (C44A) ( B ). Single representative TruSeq libraries are shown (replicate #2 in Table S1 ). CHIKV (+) strands per virus-derived small RNA in 1 ug of total RNA (calculated from normalized 25–29 nt reads identified in replicate TruSeq libraries) ( C ). Error bars indicate the standard deviation among three biological replicates. Modulation of alphavirus infection by an antiviral piwi-like RNA pathway in dcr-2 FS−1 (C6/36) cells ( D ). Time course of cytopathology in dcr-2 FS−1 (C6/36) cells infected with recombinant CHIK viruses (20X magnification).

    Journal: PLoS Pathogens

    Article Title: Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma

    doi: 10.1371/journal.ppat.1002470

    Figure Lengend Snippet: B2-mediated suppression of piRNA-like viral small RNAs in dcr-2 null mutant cells. Size distribution and nucleotide analysis of virus-derived small RNAs in dcr-2 FS−1 cells infected with CHIKV-B2 (FHV) ( A ) or CHIKV-B2 (C44A) ( B ). Single representative TruSeq libraries are shown (replicate #2 in Table S1 ). CHIKV (+) strands per virus-derived small RNA in 1 ug of total RNA (calculated from normalized 25–29 nt reads identified in replicate TruSeq libraries) ( C ). Error bars indicate the standard deviation among three biological replicates. Modulation of alphavirus infection by an antiviral piwi-like RNA pathway in dcr-2 FS−1 (C6/36) cells ( D ). Time course of cytopathology in dcr-2 FS−1 (C6/36) cells infected with recombinant CHIK viruses (20X magnification).

    Article Snippet: Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma To investigate the small RNA populations present in Aedes aegypti and Aedes albopictus mosquitoes infected with CHIKV, we prepared cDNA libraries from the ∼18–35-nt fraction of total RNA and sequenced on an Illumina platform.

    Techniques: Mutagenesis, Derivative Assay, Infection, Standard Deviation, Recombinant

    Production of piRNA-like viral small RNAs in the mosquito soma. Size distribution, density plots, and nucleotide analysis of virus-derived small RNAs in A. aegypti ( A ), A. albopictus ( B ) and head and thorax of A. albopictus ( C ) infected with CHIKV. Example weblogos are shown for the predominant size classes. Arrows denote approximate start of 26S mRNA.

    Journal: PLoS Pathogens

    Article Title: Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma

    doi: 10.1371/journal.ppat.1002470

    Figure Lengend Snippet: Production of piRNA-like viral small RNAs in the mosquito soma. Size distribution, density plots, and nucleotide analysis of virus-derived small RNAs in A. aegypti ( A ), A. albopictus ( B ) and head and thorax of A. albopictus ( C ) infected with CHIKV. Example weblogos are shown for the predominant size classes. Arrows denote approximate start of 26S mRNA.

    Article Snippet: Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma To investigate the small RNA populations present in Aedes aegypti and Aedes albopictus mosquitoes infected with CHIKV, we prepared cDNA libraries from the ∼18–35-nt fraction of total RNA and sequenced on an Illumina platform.

    Techniques: Derivative Assay, Infection

    Production of piRNA-like viral small RNAs increases during pathogenic virus infections. Size distribution and nucleotide analysis of virus-derived small RNAs in the head and thorax of A. albopictus infected with CHIKV-ΔB2 ( A ), CHIKV-B2 (NoV) ( B ), or CHIKV-B2 (FHV) ( C ). Survival of A. albopictus after infection with recombinant CHIK viruses and mock injection ( D ). Error bars indicate the standard deviation among triplicate cohorts (n = 90). Strand-specific quantitative real-time PCR analysis of CHIKV (+) strands; shown as the number of copies per virus-derived small RNA in 1ug of total RNA (calculated from normalized reads identified in the corresponding library) ( E ). Error bars indicate the standard deviation among three biological replicates.

    Journal: PLoS Pathogens

    Article Title: Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma

    doi: 10.1371/journal.ppat.1002470

    Figure Lengend Snippet: Production of piRNA-like viral small RNAs increases during pathogenic virus infections. Size distribution and nucleotide analysis of virus-derived small RNAs in the head and thorax of A. albopictus infected with CHIKV-ΔB2 ( A ), CHIKV-B2 (NoV) ( B ), or CHIKV-B2 (FHV) ( C ). Survival of A. albopictus after infection with recombinant CHIK viruses and mock injection ( D ). Error bars indicate the standard deviation among triplicate cohorts (n = 90). Strand-specific quantitative real-time PCR analysis of CHIKV (+) strands; shown as the number of copies per virus-derived small RNA in 1ug of total RNA (calculated from normalized reads identified in the corresponding library) ( E ). Error bars indicate the standard deviation among three biological replicates.

    Article Snippet: Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma To investigate the small RNA populations present in Aedes aegypti and Aedes albopictus mosquitoes infected with CHIKV, we prepared cDNA libraries from the ∼18–35-nt fraction of total RNA and sequenced on an Illumina platform.

    Techniques: Derivative Assay, Infection, Recombinant, Injection, Standard Deviation, Real-time Polymerase Chain Reaction

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Indirect mouse anti-CHIKV IgG ELISAs utilizing either (A) EILV/CHIKV or (B) cell-lysate antigen to detect serially diluted polyclonal anti-CHIKV MIAF (measured over a range of serum dilutions, red) or monoclonal antibody CHK-175 (expressed in ng quantities, blue). MIAF against Gamboa virus and MIS against mosquito antigens were included as negative controls. Mean and standard deviation of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Indirect mouse anti-CHIKV IgG ELISAs utilizing either (A) EILV/CHIKV or (B) cell-lysate antigen to detect serially diluted polyclonal anti-CHIKV MIAF (measured over a range of serum dilutions, red) or monoclonal antibody CHK-175 (expressed in ng quantities, blue). MIAF against Gamboa virus and MIS against mosquito antigens were included as negative controls. Mean and standard deviation of 2 replicates are reported.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Standard Deviation

    Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Safety of EILV/CHIKV in the newborn mouse model of neurovirulence. (A) Survival of infant mice infected IC with EILV/CHIKV, live-attenuated vaccine strain 181/25, or PBS. (B) Replication of EILV/CHIKV or strain 181/25 in newborn mouse brain tissue.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Safety of EILV/CHIKV in the newborn mouse model of neurovirulence. (A) Survival of infant mice infected IC with EILV/CHIKV, live-attenuated vaccine strain 181/25, or PBS. (B) Replication of EILV/CHIKV or strain 181/25 in newborn mouse brain tissue.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Mouse Assay, Infection

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: