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Image Search Results
Journal: Journal of Molecular Endocrinology
Article Title: C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and Pax7 expression
doi: 10.1677/jme-07-0175
Figure Lengend Snippet: Figure 6 Analysis of Pax7 expression during C2C12 cell differentiation. (A) Time-dependent analysis of Pax7 protein expression. After 2 (d2), 4 (d4), 6 (d6), and 8 (d8) days cells were fixed and stained with a Pax7-specific antibody and DAPI. The analysis of expression was performed by confocal microscopy (magnification 200!). (B) Calculation of the percentage of Pax7-positive nuclei. Indicated are the days of culture in FCS medium. *Means statistically different against day 2 (P!0.05).
Article Snippet: The following antibodies were used: myostatin MF408 (R&D Systems, Wiesbaden, Germany), anti-goat T-7028TRITC conjugate (Sigma– Aldrich), paired
Techniques: Expressing, Cell Differentiation, Staining, Confocal Microscopy
Journal: Journal of Molecular Endocrinology
Article Title: C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and Pax7 expression
doi: 10.1677/jme-07-0175
Figure Lengend Snippet: Figure 7 The effect of dihydrotestosterone (DHT) on Pax7 expression during C2C12 cell differentiation. (A) Time-dependent analysis of Pax7 protein expression. After 2 (d2), 4 (d4), 6 (d6), and 8 (d8) days, the cells were fixed and stained with a Pax7-specific antibody and DAPI (not shown). (B) Calculation of the percentage of Pax7-positive nuclei.
Article Snippet: The following antibodies were used: myostatin MF408 (R&D Systems, Wiesbaden, Germany), anti-goat T-7028TRITC conjugate (Sigma– Aldrich), paired
Techniques: Expressing, Cell Differentiation, Staining
Journal: Cell reports
Article Title: Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
doi: 10.1016/j.celrep.2020.107599
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: The following primary antibodies were used at the concentration indicated by manufacturer’s protocol: CaM Kinase II alpha (6G9) (NB100–1983), LMX1A (NBP1–81303) Novusbio; SYNAPSIN (106 001), HOMER (160 003) Synaptic System; EGFR (Ab231), FGFR1 phosphoY654 (Ab59194), TBR1 (Ab31940), REELIN (Ab18570), CYCLIN D1 (Ab10540), FGFR2 (Ab10648), BMPR1A (Ab38560) Abcam; HES1 (11988), p-SMAD1/5 (9516), CYCLIN D1 (2926), pERK1/2 (4370), FGFR1 (9740) Cell Signaling Technology; PAX6 (PRB-278P) BioLegend; NESTIN (MAB1259), OTX2 (AF1979), PDGFR alpha (AF1062; AF307), SOX2 (AF2018; MAB2018), SOX21 (AF3538), TuJ1 (MAB1195), EGFR (AF1280),
Techniques: Virus, Plasmid Preparation, Recombinant, Transfection, Antibody Labeling, In Vitro, Microarray, Gene Expression, Derivative Assay, Software
Journal: bioRxiv
Article Title: PIEZO1 and PIEZO2 Blade domains are differentially required for channel localization and function
doi: 10.1101/2024.04.06.588398
Figure Lengend Snippet: A) Immunocytochemistry of PIEZO2 ( 1–644 ) encompassing the Distal Blade (anti-GFP antibody) along with an Endoplasmic Reticulum marker ( ER-mCherry ) (anti-RFP antibody). B) Immunocytochemistry of AQP3-tGFP (1st row) and AQP3-THU3-tGFP (anti-turboGFP antibody) along with cis Golgi Marker (2nd row, Giantin-mCherry) or an ER (ER-mCherry) (3rd row, anti-RFP antibody); the inset highlights the ring-shaped accumulations of the chimeric protein.
Article Snippet: Samples were then incubated ON at 4°C with a dilution of 1/2,000 anti-GFP chicken antibody (Aves Lab; GFP-1020), or a dilution of 1/1,000
Techniques: Immunocytochemistry, Marker