Journal: Cell Biochemistry and Biophysics

Article Title: The influence of iron on selected properties of synthetic pheomelanin

doi: 10.1007/s12013-020-00918-1

Figure Lengend Snippet: 7 K EPR spectra of CDM without ( a – c ) or with ( d – f ) 1% (w/w) iron. These EPR spectra were registered after dilution of CDM to 1 mg/ml in PBS, pH 7.4 ( a , d ), 45 mM DTPA adjusted to pH 7.4 ( b , e ) or 45 mM DTPA with 5 mM KCN (resultant pH 7.8) ( c , f ) and their incubation at room temperature for 1 h (continuous line) or 24 h (dotted line)

Article Snippet: l -β-(3,4-dihydroxyphenyl)alanine ( l -DOPA), l -cysteine hydrochloride, mushroom tyrosinase, diethylenetriaminepentaacetic acid (DTPA), Chelex-100, and dialysis bags were from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany).

Techniques: Incubation

Journal: Cell Biochemistry and Biophysics

Article Title: The influence of iron on selected properties of synthetic pheomelanin

doi: 10.1007/s12013-020-00918-1

Figure Lengend Snippet: EPR spectra of iron bound to CDM. EPR spectra a – c were registered at 77 K after dilution of CDM to 1 mg/ml in PBS, pH 7.4 ( a ), 45 mM DTPA adjusted to pH 7.4 ( b ), or 45 mM DTPA with 5 mM KCN (resultant pH 7.8) ( c ) and their incubation at room temperature for 1 h (continuous line) or 24 h (dotted line). d Wide scan carried out at 20 K at CDM concentration 2 mg/ml

Article Snippet: l -β-(3,4-dihydroxyphenyl)alanine ( l -DOPA), l -cysteine hydrochloride, mushroom tyrosinase, diethylenetriaminepentaacetic acid (DTPA), Chelex-100, and dialysis bags were from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany).

Techniques: Incubation, Concentration Assay

Journal: Cell Biochemistry and Biophysics

Article Title: The influence of iron on selected properties of synthetic pheomelanin

doi: 10.1007/s12013-020-00918-1

Figure Lengend Snippet: Time-dependent changes of 77 K integrated EPR signal of CDM ( a , b ) and 350–550 nm integrated optical absorption ( c ) irradiated with 400 nm (265 mW/cm 2 ) light. EPR measurements were carried out in PBS, pH 7.4 ( a ) or in 45 mM DTPA with 5 mM KCN, pH 7.8 ( b ). Optical absorption was measured in 1 M NaOH. Filled circles—control melanin without iron, open diamonds—melanin with 1% (w/w) iron

Article Snippet: l -β-(3,4-dihydroxyphenyl)alanine ( l -DOPA), l -cysteine hydrochloride, mushroom tyrosinase, diethylenetriaminepentaacetic acid (DTPA), Chelex-100, and dialysis bags were from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany).

Techniques: Irradiation, Control

Journal: Cell Biochemistry and Biophysics

Article Title: The influence of iron on selected properties of synthetic pheomelanin

doi: 10.1007/s12013-020-00918-1

Figure Lengend Snippet: 77 K EPR spectra of iron bound to CDM photodegraded for 40 h except dotted line in c . EPR measurements were carried out after the dilution of CDM samples to 0.5 mg/ml in PBS, pH 7.4 ( a ), 45 mM DTPA with 5 mM KCN, pH 7.8 ( b ), 45 mM DTPA, pH 7.4 ( c ), water, pH 7.4 ( d ). Dotted line in c —0.1791 iron complex with citrate after addition of 45 mM DTPA. EPR signal of iron in citrate—DTPA system is multiplied by 0.2

Article Snippet: l -β-(3,4-dihydroxyphenyl)alanine ( l -DOPA), l -cysteine hydrochloride, mushroom tyrosinase, diethylenetriaminepentaacetic acid (DTPA), Chelex-100, and dialysis bags were from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany).

Techniques: