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  • 99
    Thermo Fisher chaps lysis buffer
    Chaps Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lysis buffer/product/Thermo Fisher
    Average 99 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    chaps lysis buffer - by Bioz Stars, 2020-09
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    95
    Millipore chaps lysis buffer
    Surface marker expression, SA-β-gal staining and telomerase activity in HBCEC . A . Determination of the percentage of cell surface marker expression in HBCEC at different ages. Expression of the surface marker proteins CD24, CD44, CD227 was maintained during long term culture of HBCEC. Whereas CD24 and CD44 were similarly expressed after 176d and 462d, CD227 increased from 52% to 88% in HBCEC 462d. The flow cytometry measurements varied by about 8%. B . SA-β-gal staining of primary HBCEC and HMEC cultures. Staining for SA-β-gal of a HBCEC population after 722d in culture revealed little if any positive cell. Normal HMEC in passage 16, however, displayed already predominantly enlarged senescent cells after 32d, demonstrated by the dark-green stain (bar = 200 μm). C . Telomerase (TRAP-)assay of primary cultures from breast cancer biopsies. Telomerase activity was analyzed according to the Telomeric Repeat Amplification Protocol (TRAP). HBCEC populations demonstrated telomerase activity independent of the age of the culture and the harvest method. The human embryonic kidney (HEK) 293T cell line was used as a positive control and <t>1×</t> <t>CHAPS</t> buffer served as a negative control. Quantification was performed using densitometric analysis.
    Chaps Lysis Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 596 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lysis buffer/product/Millipore
    Average 95 stars, based on 596 article reviews
    Price from $9.99 to $1999.99
    chaps lysis buffer - by Bioz Stars, 2020-09
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    94
    Cell Signaling Technology Inc chaps cell extract buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Cell Extract Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps cell extract buffer/product/Cell Signaling Technology Inc
    Average 94 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    chaps cell extract buffer - by Bioz Stars, 2020-09
    94/100 stars
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    88
    Protein Simple Inc bicine chaps lysis buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Bicine Chaps Lysis Buffer, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 88/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bicine chaps lysis buffer/product/Protein Simple Inc
    Average 88 stars, based on 114 article reviews
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    bicine chaps lysis buffer - by Bioz Stars, 2020-09
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    98
    Millipore chaps lcb 3 3 cholamidopropyl dimethylammonio 1 propanesulfonate
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Lcb 3 3 Cholamidopropyl Dimethylammonio 1 Propanesulfonate, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lcb 3 3 cholamidopropyl dimethylammonio 1 propanesulfonate/product/Millipore
    Average 98 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    chaps lcb 3 3 cholamidopropyl dimethylammonio 1 propanesulfonate - by Bioz Stars, 2020-09
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    88
    Protein Simple Inc bicine chaps buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Bicine Chaps Buffer, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 88/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bicine chaps buffer/product/Protein Simple Inc
    Average 88 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    bicine chaps buffer - by Bioz Stars, 2020-09
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    99
    Bio-Rad chaps lysis buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Lysis Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lysis buffer/product/Bio-Rad
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    chaps lysis buffer - by Bioz Stars, 2020-09
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    94
    GE Healthcare chaps w v
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps W V, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps w v/product/GE Healthcare
    Average 94 stars, based on 53 article reviews
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    chaps w v - by Bioz Stars, 2020-09
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    96
    Thermo Fisher pbs
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 45706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Thermo Fisher
    Average 96 stars, based on 45706 article reviews
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    92
    Bio-Rad chaps buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps buffer/product/Bio-Rad
    Average 92 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    chaps buffer - by Bioz Stars, 2020-09
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    93
    FIVEphoton Biochemicals chaps lysis buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Lysis Buffer, supplied by FIVEphoton Biochemicals, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lysis buffer/product/FIVEphoton Biochemicals
    Average 93 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    chaps lysis buffer - by Bioz Stars, 2020-09
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    92
    Merck KGaA chaps lysis buffer
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps Lysis Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps lysis buffer/product/Merck KGaA
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    chaps lysis buffer - by Bioz Stars, 2020-09
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    94
    Bio-Rad chaps w v
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chaps W V, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chaps w v/product/Bio-Rad
    Average 94 stars, based on 12 article reviews
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    chaps w v - by Bioz Stars, 2020-09
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    88
    Thermo Fisher chapso
    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in <t>CHAPS</t> buffer. Rabbit <t>IgG</t> and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates
    Chapso, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chapso/product/Thermo Fisher
    Average 88 stars, based on 88 article reviews
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    Image Search Results


    Surface marker expression, SA-β-gal staining and telomerase activity in HBCEC . A . Determination of the percentage of cell surface marker expression in HBCEC at different ages. Expression of the surface marker proteins CD24, CD44, CD227 was maintained during long term culture of HBCEC. Whereas CD24 and CD44 were similarly expressed after 176d and 462d, CD227 increased from 52% to 88% in HBCEC 462d. The flow cytometry measurements varied by about 8%. B . SA-β-gal staining of primary HBCEC and HMEC cultures. Staining for SA-β-gal of a HBCEC population after 722d in culture revealed little if any positive cell. Normal HMEC in passage 16, however, displayed already predominantly enlarged senescent cells after 32d, demonstrated by the dark-green stain (bar = 200 μm). C . Telomerase (TRAP-)assay of primary cultures from breast cancer biopsies. Telomerase activity was analyzed according to the Telomeric Repeat Amplification Protocol (TRAP). HBCEC populations demonstrated telomerase activity independent of the age of the culture and the harvest method. The human embryonic kidney (HEK) 293T cell line was used as a positive control and 1× CHAPS buffer served as a negative control. Quantification was performed using densitometric analysis.

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Characterization of human breast cancer epithelial cells (HBCEC) derived from long term cultured biopsies

    doi: 10.1186/1756-9966-28-127

    Figure Lengend Snippet: Surface marker expression, SA-β-gal staining and telomerase activity in HBCEC . A . Determination of the percentage of cell surface marker expression in HBCEC at different ages. Expression of the surface marker proteins CD24, CD44, CD227 was maintained during long term culture of HBCEC. Whereas CD24 and CD44 were similarly expressed after 176d and 462d, CD227 increased from 52% to 88% in HBCEC 462d. The flow cytometry measurements varied by about 8%. B . SA-β-gal staining of primary HBCEC and HMEC cultures. Staining for SA-β-gal of a HBCEC population after 722d in culture revealed little if any positive cell. Normal HMEC in passage 16, however, displayed already predominantly enlarged senescent cells after 32d, demonstrated by the dark-green stain (bar = 200 μm). C . Telomerase (TRAP-)assay of primary cultures from breast cancer biopsies. Telomerase activity was analyzed according to the Telomeric Repeat Amplification Protocol (TRAP). HBCEC populations demonstrated telomerase activity independent of the age of the culture and the harvest method. The human embryonic kidney (HEK) 293T cell line was used as a positive control and 1× CHAPS buffer served as a negative control. Quantification was performed using densitometric analysis.

    Article Snippet: Briefly, HBCEC and 293T control cells were washed with ice-cold PBS and homogenized in 100 μl ice-cold 1× CHAPS lysis buffer (Chemicon).

    Techniques: Marker, Expressing, Staining, Activity Assay, Flow Cytometry, Cytometry, TRAP Assay, Amplification, Positive Control, Negative Control

    N4BP1 overexpression inhibits 9F7-F11-mediated HER3 ubiquitination and degradation induced by ITCH, and promotes HER3 protein stabilization in BxPc3 cells ( A ) Cells were co-transfected with the Myc-ITCHwt, HA-Ub and/or GFP-N4BP1 plasmids for 24 hr. Transfected cells were then incubated with 20 μM MG132 for 5 hr before addition of 50 μg/mL 9F7-F11 for 3 hr. After cells lysis with the CHAPS buffer and immunoprecipitation with the HER3 Ab, the ubiquitination status was analyzed by western blotting using an anti-HA antibody. HER3 and ITCH were detected using specific antibodies. ( B ) Cells were transfected with increasing doses of the V5-N4BP1 plasmid for 24 hr, and then incubated with 50 μg/mL 9F7-F11 or with 100 ng/mL NRG-1β for 3 hr. HER3 expression was assessed in whole cell lysates by western blotting. The V5-HRP antibody was used to detect N4BP1, and β-actin was the loading control. The signal intensity (SI) of the different bands was quantified with ImageJ. WCL, whole cell lysates.

    Journal: Oncotarget

    Article Title: The anti-HER3 (ErbB3) therapeutic antibody 9F7-F11 induces HER3 ubiquitination and degradation in tumors through JNK1/2- dependent ITCH/AIP4 activation

    doi: 10.18632/oncotarget.9455

    Figure Lengend Snippet: N4BP1 overexpression inhibits 9F7-F11-mediated HER3 ubiquitination and degradation induced by ITCH, and promotes HER3 protein stabilization in BxPc3 cells ( A ) Cells were co-transfected with the Myc-ITCHwt, HA-Ub and/or GFP-N4BP1 plasmids for 24 hr. Transfected cells were then incubated with 20 μM MG132 for 5 hr before addition of 50 μg/mL 9F7-F11 for 3 hr. After cells lysis with the CHAPS buffer and immunoprecipitation with the HER3 Ab, the ubiquitination status was analyzed by western blotting using an anti-HA antibody. HER3 and ITCH were detected using specific antibodies. ( B ) Cells were transfected with increasing doses of the V5-N4BP1 plasmid for 24 hr, and then incubated with 50 μg/mL 9F7-F11 or with 100 ng/mL NRG-1β for 3 hr. HER3 expression was assessed in whole cell lysates by western blotting. The V5-HRP antibody was used to detect N4BP1, and β-actin was the loading control. The signal intensity (SI) of the different bands was quantified with ImageJ. WCL, whole cell lysates.

    Article Snippet: Cell lysis and immunoprecipitation Cells were lysed in CHAPS buffer (Sigma-Aldrich) containing the protease inhibitor cocktail V (Calbiochem, Billerica, MA) and the phosphatase inhibitor cocktail II (Sigma-Aldrich).

    Techniques: Over Expression, Transfection, Incubation, Lysis, Immunoprecipitation, Western Blot, Plasmid Preparation, Expressing

    Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in CHAPS buffer. Rabbit IgG and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates

    Journal: Molecular and Cellular Biology

    Article Title: Pseudomonas Exotoxin A-Mediated Apoptosis Is Bak Dependent and Preceded by the Degradation of Mcl-1 ▿

    doi: 10.1128/MCB.00813-09

    Figure Lengend Snippet: Mcl-1 and Bcl-x L bound to Bak in MEFs, and overexpression protected MEFs from PE-induced cell death. (A) WT MEF lysates were prepared in CHAPS buffer. Rabbit IgG and antibodies against Bcl-2, Mcl-1, and Bcl-x L were used for immunoprecipitation. Immunoprecipitates

    Article Snippet: Cell lysates (100 μl) were diluted with 300 μl 1% CHAPS buffer; mixed with rabbit IgG, rabbit anti Bcl-2 (Cell Signaling; no. 2870), rabbit anti Bcl-xL , and rabbit anti Mcl-1 antibodies; and incubated in a cold room overnight with gentle agitation.

    Techniques: Over Expression, Immunoprecipitation

    Bak released from suppressor complexes after degradation of Mcl-1. (A) WT MEFs were treated with 50 ng/ml of PE for 20 h. Cell lysates were prepared with 1% CHAPS buffer and immunoprecipitated with anti-Mcl-1 antibody (Ab) (M) or anti-Bcl-x L antibody

    Journal: Molecular and Cellular Biology

    Article Title: Pseudomonas Exotoxin A-Mediated Apoptosis Is Bak Dependent and Preceded by the Degradation of Mcl-1 ▿

    doi: 10.1128/MCB.00813-09

    Figure Lengend Snippet: Bak released from suppressor complexes after degradation of Mcl-1. (A) WT MEFs were treated with 50 ng/ml of PE for 20 h. Cell lysates were prepared with 1% CHAPS buffer and immunoprecipitated with anti-Mcl-1 antibody (Ab) (M) or anti-Bcl-x L antibody

    Article Snippet: Cell lysates (100 μl) were diluted with 300 μl 1% CHAPS buffer; mixed with rabbit IgG, rabbit anti Bcl-2 (Cell Signaling; no. 2870), rabbit anti Bcl-xL , and rabbit anti Mcl-1 antibodies; and incubated in a cold room overnight with gentle agitation.

    Techniques: Immunoprecipitation