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Image Search Results
Journal: Nature Communications
Article Title: The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors
doi: 10.1038/s41467-017-01686-y
Figure Lengend Snippet: CXCR3 and LRP1 expression in glioblastoma samples from patients. a HE staining was performed in the experimental mouse intracranial P3 GBM model that recapitulates the regional heterogeneity of gliomas. (1) healthy brain, (2) angiogenic part, and (3) invasive part. b Immunofluorescence with anti-vimentin (sc-6260 clone V9, Santa Cruz) ( λ = 547 nm) and anti-LRP1 (antibody 2703-1 for LRP1β, Epitomics) ( λ = 488 nm) antibodies on tissue from healthy mouse brain, angiogenic, or invasive part of the P3 GBM tumor. Scale: 5 µm. c Graph depicts the quantification of LRP1 expression in angiogenic and invasive areas ( n = 30). *** P <0.001. d MRI image from a representative glioblastoma (GBM) patient. Samples were taken intraoperatively with the aid of neuronavigation from the tumor angiogenic core (contrast enhancement ring) and the infiltrative area (T1 hypointense abnormality). e Immunofluorescence with anti-CXCR3 (antibody 2ar1, Abcam) and anti-LRP1 antibodies (antibody 2703-1 for LRP1β, Epitomics) ( λ = 488 nm) on tissue samples from either the angiogenic or invasive part of the tumor. Membrane and/or perinuclear localization of CXCR3 and LRP1 were observed at a ×630 magnification with phase contrast brightfield under a confocal laser scanning microscope (Nikon Eclipse ti). Scale: 5 μm. Graphs (protein intensity, n = 30, and CXCR3 localization, n > 300) depict the quantification from three different patients. NS non-significant. *** P <0.001. Statistical comparison between two groups was performed using the Mann–Whitney U -test. Multiple comparisons were performed with one-way analysis of variance, followed by Bonferroni post hoc tests
Article Snippet: All cells used for phenotypic and functional studies have been further characterized more in detail by
Techniques: Expressing, Staining, Immunofluorescence, Membrane, Laser-Scanning Microscopy, Comparison, MANN-WHITNEY