Journal: Pharmaceutical Biology

Article Title: Chlorogenic acid promotes angiogenesis and attenuates apoptosis following cerebral ischaemia-reperfusion injury by regulating the PI3K-Akt signalling

doi: 10.1080/13880209.2022.2110599

Figure Lengend Snippet: CGA regulated OGD/R-induced apoptosis of HBMECs. (A) The cell viability was evaluated by CCK-8 assay in HBMECs after stimulation with different concentrations of CGA for 28 h. HBMECs were subjected to OGD/R and CGA treatment. (B) The cell viability was evaluated by CCK-8 assay. (C–E) The apoptosis was investigated by flow cytometry and TUNEL assays. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For CGA treatment, HBMECs were coped with 20, 40, 80 or 160 μM CGA (purity: 99.55%; MedChemExpress, Monmouth Junction, NJ, USA) for 28 h. CGA was added meanwhile at the start of OGD and maintained in the culture medium throughout the OGD and reoxygenation.

Techniques: CCK-8 Assay, Flow Cytometry, TUNEL Assay

Journal: Pharmaceutical Biology

Article Title: Chlorogenic acid promotes angiogenesis and attenuates apoptosis following cerebral ischaemia-reperfusion injury by regulating the PI3K-Akt signalling

doi: 10.1080/13880209.2022.2110599

Figure Lengend Snippet: CGA regulated angiogenesis of HBMECs under OGD/R damage. HBMECs were treated with OGD/R and indicated concentrations of CGA. (A) The angiogenesis was estimated by tube formation assay. (B) The VEGFA abundance was tested by western blot. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For CGA treatment, HBMECs were coped with 20, 40, 80 or 160 μM CGA (purity: 99.55%; MedChemExpress, Monmouth Junction, NJ, USA) for 28 h. CGA was added meanwhile at the start of OGD and maintained in the culture medium throughout the OGD and reoxygenation.

Techniques: Tube Formation Assay, Western Blot

Journal: Pharmaceutical Biology

Article Title: Chlorogenic acid promotes angiogenesis and attenuates apoptosis following cerebral ischaemia-reperfusion injury by regulating the PI3K-Akt signalling

doi: 10.1080/13880209.2022.2110599

Figure Lengend Snippet: CGA regulated activation of the PI3K-Akt signalling in HBMECs under OGD/R damage. HBMECs were treated with 30 μM of 740Y-P or 25 μM of LY294002 for 24 h prior to treatment with OGD/R and 80 μM of CGA. (A) The abundance of PI3K, p-PI3K, Akt and p-Akt was detected by western blots. (B, C) The p-PI3K/PI3K and p-Akt/Akt levels were estimated. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For CGA treatment, HBMECs were coped with 20, 40, 80 or 160 μM CGA (purity: 99.55%; MedChemExpress, Monmouth Junction, NJ, USA) for 28 h. CGA was added meanwhile at the start of OGD and maintained in the culture medium throughout the OGD and reoxygenation.

Techniques: Activation Assay, Western Blot

Journal: Pharmaceutical Biology

Article Title: Chlorogenic acid promotes angiogenesis and attenuates apoptosis following cerebral ischaemia-reperfusion injury by regulating the PI3K-Akt signalling

doi: 10.1080/13880209.2022.2110599

Figure Lengend Snippet: LY294002 regulated the effect of CGA on apoptosis. HBMECs were treated with 25 μM of LY294002 for 24 h prior to treatment with OGD/R and 80 μM of CGA. (A) The cell viability was measured by CCK-8 assay. (B–D) The apoptosis was confirmed by flow cytometry and TUNEL assays. ** p < 0.01; *** p < 0.001.

Article Snippet: For CGA treatment, HBMECs were coped with 20, 40, 80 or 160 μM CGA (purity: 99.55%; MedChemExpress, Monmouth Junction, NJ, USA) for 28 h. CGA was added meanwhile at the start of OGD and maintained in the culture medium throughout the OGD and reoxygenation.

Techniques: CCK-8 Assay, Flow Cytometry, TUNEL Assay

Journal: Pharmaceutical Biology

Article Title: Chlorogenic acid promotes angiogenesis and attenuates apoptosis following cerebral ischaemia-reperfusion injury by regulating the PI3K-Akt signalling

doi: 10.1080/13880209.2022.2110599

Figure Lengend Snippet: LY294002 reversed the effect of CGA on angiogenesis. HBMECs were stimulated with 25 μM of LY294002 for 24 h before treatment with OGD/R and 80 μM of CGA. (A) Angiogenesis was quantified by tube formation assay. (B) The VEGFA content was detected by western blots. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For CGA treatment, HBMECs were coped with 20, 40, 80 or 160 μM CGA (purity: 99.55%; MedChemExpress, Monmouth Junction, NJ, USA) for 28 h. CGA was added meanwhile at the start of OGD and maintained in the culture medium throughout the OGD and reoxygenation.

Techniques: Tube Formation Assay, Western Blot

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts

doi: 10.1007/s10815-023-02773-4

Figure Lengend Snippet: List of primers used for quantitative PCR

Article Snippet: CGA , Glycoprotein hormones, alpha polypeptide , Hs00985275_g1.

Techniques: Binding Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts

doi: 10.1007/s10815-023-02773-4

Figure Lengend Snippet: Generation of TR-like cells through 5-aza-CR exposure and trophoblast induction. Fibroblasts exposed to 5-aza-CR lost their typical elongated shape (untreated fibroblasts) and became smaller with larger nuclei and granular cytoplasm (post 5-aza-CR) (scale bars 100 µm; A ). At day 11 of trophoblast induction, cells acquired a tight adherent epithelial morphology, exhibiting round or ellipsoid shape, with round nuclei and well-defined borders (trophoblast-like cells; scale bar 100 µm; A ). Immunostainings show cell positivity for the TR markers CDX2 (red, left panel) and KRT19 (green, right panel). Nuclei are stained in blue (scale bars 100 μm; B ). Transcription levels for pluripotent- ( OCT4 , NANOG , REX1 , SOX2 , KLF17 , PRDM14 , DPPA2 ), fibroblast- ( VIM , THY1 ), and TR- related genes ( GCM1 , CGA , CGB , HSD17B1 , CYP11A1 , PGF , ESRRB , GATA2 , GATA3 , KRT19 ) in untreated fibroblasts (white bars), fibroblasts exposed to 5-aza-CR (Post 5-aza-CR, black bars), at day 11 of trophoblast induction (trophoblast-like cells, grey bars) and in JAR cell line (JAR, blue bars). Gene expression values are reported with the highest expression set to 1 and all others relative to this. Different superscripts denote significant differences ( P < 0.05; C )

Article Snippet: CGA , Glycoprotein hormones, alpha polypeptide , Hs00985275_g1.

Techniques: Staining, Gene Expression, Expressing

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts

doi: 10.1007/s10815-023-02773-4

Figure Lengend Snippet: Production of epiBlastoids by assembling TR-like cells and ICM-like spheroids. Representative image of an epiBlastoid (scale bar 50 μm; A ). Rates of epiBlastoids displaying diameters ranging from 100 to 150 μm and from 151 to 200 μm *Superscripts denote significant differences ( P < 0.05; B ). Immunostaining showing CDX2 + cells (red) localize in out layer of the epiBlastoids and OCT4 + cells (green) in the inner compartment. Nuclei are stained in blue (scale bars 50 μm; C ). YAP protein is confined in the nuclear compartment of TROP2 + cells, while it is excluded from the nucleus and shifts into the cytoplasm in TROP2 − cells. Nuclei are stained in blue (scale bars 100 μm; D ). Densitometric analysis of western blots for YAP protein in cytoplasm (C) and nucleus (N) of TROP2 + (black bars) and TROP2 − cells (white bars). The values (arbitrary units) are reported as relative optical density of the bands normalized to GAPDH. *Superscripts denote significant differences ( P < 0.05; E ). Representative YAP and GAPDH western blots for the two cell compartments of each cell type are also shown ( F ). Transcription levels for pluripotency- ( OCT4 , NANOG , REX1 , SOX2 , KLF17 , PRDM14 , DPPA2 ) and TR-related genes ( GCM1 , CGA , CGB , HSD17B1 , CYP11A1 , PGF , ESRRB , GATA2 , GATA3 , KRT19 ) in TROP2 + cells (black bars), TROP2. − cells (white bars), human ESC (ESC, green bars), and JAR cell line (JAR, blue bars). Gene expression values are reported with the highest expression set to 1 and all others relative to this ( G )

Article Snippet: CGA , Glycoprotein hormones, alpha polypeptide , Hs00985275_g1.

Techniques: Immunostaining, Staining, Western Blot, Gene Expression, Expressing