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  • 93
    fluidigm bio rad cfx cfx
    Bio Rad Cfx Cfx, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio rad cfx cfx/product/fluidigm
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    cfx  (Bio-Rad)
    99
    Bio-Rad cfx
    Expression profile of the selected genes in the hippocampi of the tested mouse variants that was obtained by real-time <t>PCR</t> analysis. The results are presented as scatter plot; each circle corresponds to one animal. The obtained results suggest an up-regulation of the expression of Hes-5 and Strap . The data are presented as a fold change (2 −∆∆Ct ) that was normalized to the reference gene ( Uba-2 ), the statistics was estimated by <t>CFX</t> software (Bio-Rad) using post hoc method (Tukey’s test).
    Cfx, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx/product/Bio-Rad
    Average 99 stars, based on 7309 article reviews
    Price from $9.99 to $1999.99
    cfx - by Bioz Stars, 2020-05
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    99
    Bio-Rad cfx manager
    Validation of ASQ genotyping of the nr3c1 locus with restriction fragment length polymorphism (RFLP). (A) nr3c1 allele discrimination plot was generated by <t>CFX</t> Manager software. A total of 40 samples were used for this validation. Clustering of alleles visually assists allele scoring. Two no-template controls (NTCs) were used that clustered near the origin of the graph. Six samples of a serial dilution of wild-type (WT) genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, 81-, and 243-fold dilution). The samples with the lowest concentrations (81- and 243-fold dilution) did not produce a valid signal, resulting in two other unscored data points (black diamond) on the graph. *The sample 25 evaporated during ASQ reaction and was discarded from ASQ genotype scoring. The original allele calls and standard curve are available in Supplementary Fig. S4 . (B) nr3c1 allele scoring with RFLP. The total amplicon is 685 bp with two restriction sites for the restriction endonuclease, PvuII -HF. A homozygous allele gives 383 and 155 bp bands, a heterozygous allele 383, 241, 155, and 142 bp, and a WT allele 241, 155, and 142 bp. L, molecular ladder; x, empty well. (C) <t>qPCR</t> amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.
    Cfx Manager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx manager/product/Bio-Rad
    Average 99 stars, based on 3499 article reviews
    Price from $9.99 to $1999.99
    cfx manager - by Bioz Stars, 2020-05
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    99
    Bio-Rad cfx maestro
    Validation of ASQ genotyping of the nr3c1 locus with restriction fragment length polymorphism (RFLP). (A) nr3c1 allele discrimination plot was generated by <t>CFX</t> Manager software. A total of 40 samples were used for this validation. Clustering of alleles visually assists allele scoring. Two no-template controls (NTCs) were used that clustered near the origin of the graph. Six samples of a serial dilution of wild-type (WT) genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, 81-, and 243-fold dilution). The samples with the lowest concentrations (81- and 243-fold dilution) did not produce a valid signal, resulting in two other unscored data points (black diamond) on the graph. *The sample 25 evaporated during ASQ reaction and was discarded from ASQ genotype scoring. The original allele calls and standard curve are available in Supplementary Fig. S4 . (B) nr3c1 allele scoring with RFLP. The total amplicon is 685 bp with two restriction sites for the restriction endonuclease, PvuII -HF. A homozygous allele gives 383 and 155 bp bands, a heterozygous allele 383, 241, 155, and 142 bp, and a WT allele 241, 155, and 142 bp. L, molecular ladder; x, empty well. (C) <t>qPCR</t> amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.
    Cfx Maestro, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx maestro/product/Bio-Rad
    Average 99 stars, based on 78 article reviews
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    cfx maestro - by Bioz Stars, 2020-05
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    97
    Bio-Rad cfx manager v3 1
    Validation of ASQ genotyping of the nr3c1 locus with restriction fragment length polymorphism (RFLP). (A) nr3c1 allele discrimination plot was generated by <t>CFX</t> Manager software. A total of 40 samples were used for this validation. Clustering of alleles visually assists allele scoring. Two no-template controls (NTCs) were used that clustered near the origin of the graph. Six samples of a serial dilution of wild-type (WT) genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, 81-, and 243-fold dilution). The samples with the lowest concentrations (81- and 243-fold dilution) did not produce a valid signal, resulting in two other unscored data points (black diamond) on the graph. *The sample 25 evaporated during ASQ reaction and was discarded from ASQ genotype scoring. The original allele calls and standard curve are available in Supplementary Fig. S4 . (B) nr3c1 allele scoring with RFLP. The total amplicon is 685 bp with two restriction sites for the restriction endonuclease, PvuII -HF. A homozygous allele gives 383 and 155 bp bands, a heterozygous allele 383, 241, 155, and 142 bp, and a WT allele 241, 155, and 142 bp. L, molecular ladder; x, empty well. (C) <t>qPCR</t> amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.
    Cfx Manager V3 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx manager v3 1/product/Bio-Rad
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    Image Search Results


    Expression profile of the selected genes in the hippocampi of the tested mouse variants that was obtained by real-time PCR analysis. The results are presented as scatter plot; each circle corresponds to one animal. The obtained results suggest an up-regulation of the expression of Hes-5 and Strap . The data are presented as a fold change (2 −∆∆Ct ) that was normalized to the reference gene ( Uba-2 ), the statistics was estimated by CFX software (Bio-Rad) using post hoc method (Tukey’s test).

    Journal: International Journal of Molecular Sciences

    Article Title: Changes in Calcium Homeostasis and Gene Expression Implicated in Epilepsy in Hippocampi of Mice Overexpressing ORAI1

    doi: 10.3390/ijms20225539

    Figure Lengend Snippet: Expression profile of the selected genes in the hippocampi of the tested mouse variants that was obtained by real-time PCR analysis. The results are presented as scatter plot; each circle corresponds to one animal. The obtained results suggest an up-regulation of the expression of Hes-5 and Strap . The data are presented as a fold change (2 −∆∆Ct ) that was normalized to the reference gene ( Uba-2 ), the statistics was estimated by CFX software (Bio-Rad) using post hoc method (Tukey’s test).

    Article Snippet: The reactions were performed using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Software

    Expression profile of selected genes in hippocampi of the tested mouse variants. ( A ) Rea-time PCR analysis of the selected genes that was based on RNAseq data. The results are presented as scatter plot, each circle corresponds to one animal. Black circle corresponds to each tested wild-type probe, whereas green corresponds to transgenic ones. The obtained results suggest a down-regulation of expression of Arx , Cdkl5, and Dclk1 . The data are presented as a fold change (2 −∆∆Ct ) normalized to the three reference genes ( Uba-2 , Gapdh , Actin ). The statistics was estimated by CFX software (Bio-Rad) using the post hoc method (Tukey’s test). ( B ) The ddPCR analysis of Arx , Cdkl5 , Dclk1 using customized assays (Bio-Rad). The number of transcript copies of the selected genes are presented in relation to 1000 copies of the reference gene Uba-2 . The results are presented as dot plot, where each sign corresponds to an individual animal (3 wild-type, 6 transgenic). The statistics were calculated by GraphPad Prism software using unpaired t -test. The number of Arx transcripts was about 30 times lower than that of Cdkl5 and Dclk1 , therefore, it is presented on a separate chart.

    Journal: International Journal of Molecular Sciences

    Article Title: Changes in Calcium Homeostasis and Gene Expression Implicated in Epilepsy in Hippocampi of Mice Overexpressing ORAI1

    doi: 10.3390/ijms20225539

    Figure Lengend Snippet: Expression profile of selected genes in hippocampi of the tested mouse variants. ( A ) Rea-time PCR analysis of the selected genes that was based on RNAseq data. The results are presented as scatter plot, each circle corresponds to one animal. Black circle corresponds to each tested wild-type probe, whereas green corresponds to transgenic ones. The obtained results suggest a down-regulation of expression of Arx , Cdkl5, and Dclk1 . The data are presented as a fold change (2 −∆∆Ct ) normalized to the three reference genes ( Uba-2 , Gapdh , Actin ). The statistics was estimated by CFX software (Bio-Rad) using the post hoc method (Tukey’s test). ( B ) The ddPCR analysis of Arx , Cdkl5 , Dclk1 using customized assays (Bio-Rad). The number of transcript copies of the selected genes are presented in relation to 1000 copies of the reference gene Uba-2 . The results are presented as dot plot, where each sign corresponds to an individual animal (3 wild-type, 6 transgenic). The statistics were calculated by GraphPad Prism software using unpaired t -test. The number of Arx transcripts was about 30 times lower than that of Cdkl5 and Dclk1 , therefore, it is presented on a separate chart.

    Article Snippet: The reactions were performed using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad).

    Techniques: Expressing, Polymerase Chain Reaction, Transgenic Assay, Software

    Validation of ASQ genotyping of the nr3c1 locus with restriction fragment length polymorphism (RFLP). (A) nr3c1 allele discrimination plot was generated by CFX Manager software. A total of 40 samples were used for this validation. Clustering of alleles visually assists allele scoring. Two no-template controls (NTCs) were used that clustered near the origin of the graph. Six samples of a serial dilution of wild-type (WT) genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, 81-, and 243-fold dilution). The samples with the lowest concentrations (81- and 243-fold dilution) did not produce a valid signal, resulting in two other unscored data points (black diamond) on the graph. *The sample 25 evaporated during ASQ reaction and was discarded from ASQ genotype scoring. The original allele calls and standard curve are available in Supplementary Fig. S4 . (B) nr3c1 allele scoring with RFLP. The total amplicon is 685 bp with two restriction sites for the restriction endonuclease, PvuII -HF. A homozygous allele gives 383 and 155 bp bands, a heterozygous allele 383, 241, 155, and 142 bp, and a WT allele 241, 155, and 142 bp. L, molecular ladder; x, empty well. (C) qPCR amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.

    Journal: Human Gene Therapy

    Article Title: Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping

    doi: 10.1089/hum.2016.011

    Figure Lengend Snippet: Validation of ASQ genotyping of the nr3c1 locus with restriction fragment length polymorphism (RFLP). (A) nr3c1 allele discrimination plot was generated by CFX Manager software. A total of 40 samples were used for this validation. Clustering of alleles visually assists allele scoring. Two no-template controls (NTCs) were used that clustered near the origin of the graph. Six samples of a serial dilution of wild-type (WT) genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, 81-, and 243-fold dilution). The samples with the lowest concentrations (81- and 243-fold dilution) did not produce a valid signal, resulting in two other unscored data points (black diamond) on the graph. *The sample 25 evaporated during ASQ reaction and was discarded from ASQ genotype scoring. The original allele calls and standard curve are available in Supplementary Fig. S4 . (B) nr3c1 allele scoring with RFLP. The total amplicon is 685 bp with two restriction sites for the restriction endonuclease, PvuII -HF. A homozygous allele gives 383 and 155 bp bands, a heterozygous allele 383, 241, 155, and 142 bp, and a WT allele 241, 155, and 142 bp. L, molecular ladder; x, empty well. (C) qPCR amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.

    Article Snippet: Taking advantage of generic software provided with a qPCR machine, we used an allele discrimination plot automatically constructed by CFX Manager.

    Techniques: Generated, Software, Serial Dilution, Amplification, Real-time Polymerase Chain Reaction, Mutagenesis

    Validation of ASQ genotyping of the nod2 locus with RFLP. (A) nod2 allele discrimination plot was generated by CFX Manager software. A total of 88 samples were used for this validation. Samples 9–16 were omitted to make a row of space for the standards and NTCs in a 96-well PCR plate. Clustering of alleles visually assists allele scoring. Three NTCs were used that clustered near the origin of the graph. Five samples of a serial dilution of WT genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, and 81-fold dilution). The samples with the lowest concentration (81-fold) did not produce a valid signal, resulting in another unscored data point (black diamond) on the graph. The original allele calls and standard curve are available in Supplementary Fig. S5 . (B) nod2 allele scoring with RFLP. The total amplicon is 545 bp with one restriction site for the restriction endonuclease Xba I. A homozygous allele gives a 545 bp band, a heterozygous allele 545, 326, and 219 bp, and a WT allele 326 and 219 bp. L, molecular ladder; +, positive digestion control with endonuclease ( Xba I); −, negative digestion control without endonuclease; x, empty well. (C) qPCR amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.

    Journal: Human Gene Therapy

    Article Title: Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping

    doi: 10.1089/hum.2016.011

    Figure Lengend Snippet: Validation of ASQ genotyping of the nod2 locus with RFLP. (A) nod2 allele discrimination plot was generated by CFX Manager software. A total of 88 samples were used for this validation. Samples 9–16 were omitted to make a row of space for the standards and NTCs in a 96-well PCR plate. Clustering of alleles visually assists allele scoring. Three NTCs were used that clustered near the origin of the graph. Five samples of a serial dilution of WT genomic DNA were used for quality control (3×; 1-, 3-, 9-, 27-, and 81-fold dilution). The samples with the lowest concentration (81-fold) did not produce a valid signal, resulting in another unscored data point (black diamond) on the graph. The original allele calls and standard curve are available in Supplementary Fig. S5 . (B) nod2 allele scoring with RFLP. The total amplicon is 545 bp with one restriction site for the restriction endonuclease Xba I. A homozygous allele gives a 545 bp band, a heterozygous allele 545, 326, and 219 bp, and a WT allele 326 and 219 bp. L, molecular ladder; +, positive digestion control with endonuclease ( Xba I); −, negative digestion control without endonuclease; x, empty well. (C) qPCR amplification plot. FAM plot (blue) shows amplifications of a WT allele, whereas HEX plot (dark green) shows amplifications of a mutant allele.

    Article Snippet: Taking advantage of generic software provided with a qPCR machine, we used an allele discrimination plot automatically constructed by CFX Manager.

    Techniques: Generated, Software, Polymerase Chain Reaction, Serial Dilution, Concentration Assay, Amplification, Real-time Polymerase Chain Reaction, Mutagenesis

    Representative amplification plot using the CFX Manager™ software (Biorad, version 3.0) for one gene ( 18S ) in the absence and presence of various amounts of AuNPs. Before the addition of AuNPs, blue represents 0%; After the addition of AuNPs, green represents 25%; orange represents 50%; red represents 75% AuNPs; Without DNA, yellow represents the non-template control (NTC)

    Journal: Journal of Nanobiotechnology

    Article Title: The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

    doi: 10.1186/s12951-017-0299-9

    Figure Lengend Snippet: Representative amplification plot using the CFX Manager™ software (Biorad, version 3.0) for one gene ( 18S ) in the absence and presence of various amounts of AuNPs. Before the addition of AuNPs, blue represents 0%; After the addition of AuNPs, green represents 25%; orange represents 50%; red represents 75% AuNPs; Without DNA, yellow represents the non-template control (NTC)

    Article Snippet: Assessment of the amplification of the reference genes, as influenced by AuNPs, included use of the CFX Manager software to obtain the PCR efficiency, the linearity of the assay as well as the slope obtained from the standard curve.

    Techniques: Amplification, Software