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Image Search Results
Journal: eLife
Article Title: EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry
doi: 10.7554/elife.81288
Figure Lengend Snippet: Figure 9. EHD2 regulates SOCE through STIM1-Orai1. (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted
Article Snippet:
Techniques: Clinical Proteomics, Membrane, Microscopy, Control, Western Blot