ceramide c6 Search Results


c6ceramide  (Larodan)
90
Larodan c6ceramide
C6ceramide, supplied by Larodan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc05968438__oc7b00582_si_001-3-199-203?v=Larodan
Average 90 stars, based on 1 article reviews
c6ceramide - by Bioz Stars, 2026-07
90/100 stars
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nbd c6 ceramide  (Biotium)
93
Biotium nbd c6 ceramide
Nbd C6 Ceramide, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pm34920667__nn1c09456_si_001-12-4-16?v=Biotium
Average 93 stars, based on 1 article reviews
nbd c6 ceramide - by Bioz Stars, 2026-07
93/100 stars
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erk  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology erk
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
Erk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc05810207-103-42-49?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
erk - by Bioz Stars, 2026-07
93/100 stars
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c6 ceramide  (Croda International Plc)
95
Croda International Plc c6 ceramide
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
C6 Ceramide, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc10058238-157-0-1?v=Croda+International+Plc
Average 95 stars, based on 1 article reviews
c6 ceramide - by Bioz Stars, 2026-07
95/100 stars
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nbd sphingomyelin 6 0  (Croda International Plc)
94
Croda International Plc nbd sphingomyelin 6 0
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
Nbd Sphingomyelin 6 0, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/bio_rxiv__2025__10__13__682155-273-34-36?v=Croda+International+Plc
Average 94 stars, based on 1 article reviews
nbd sphingomyelin 6 0 - by Bioz Stars, 2026-07
94/100 stars
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igg primary antibody against human connexin 40  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology igg primary antibody against human connexin 40
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
Igg Primary Antibody Against Human Connexin 40, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc12259691-45-23-32?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
igg primary antibody against human connexin 40 - by Bioz Stars, 2026-07
93/100 stars
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nitro 2 1 3 benzoxadiazol 4 yl amino hexanoyl d galactosyl β1  (Croda International Plc)
90
Croda International Plc nitro 2 1 3 benzoxadiazol 4 yl amino hexanoyl d galactosyl β1
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
Nitro 2 1 3 Benzoxadiazol 4 Yl Amino Hexanoyl D Galactosyl β1, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/us09453835-129-110-116?v=Croda+International+Plc
Average 90 stars, based on 1 article reviews
nitro 2 1 3 benzoxadiazol 4 yl amino hexanoyl d galactosyl β1 - by Bioz Stars, 2026-07
90/100 stars
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fatty acyl  (Croda International Plc)
92
Croda International Plc fatty acyl
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
Fatty Acyl, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc02856296-44-0-33?v=Croda+International+Plc
Average 92 stars, based on 1 article reviews
fatty acyl - by Bioz Stars, 2026-07
92/100 stars
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c6 nbd lactosylceramide  (Croda International Plc)
90
Croda International Plc c6 nbd lactosylceramide
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
C6 Nbd Lactosylceramide, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pmc05722210-52-8-15?v=Croda+International+Plc
Average 90 stars, based on 1 article reviews
c6 nbd lactosylceramide - by Bioz Stars, 2026-07
90/100 stars
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n d m e t h o d s chemicals 1 acyl  (Avanti Polar)
90
Avanti Polar n d m e t h o d s chemicals 1 acyl
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
N D M E T H O D S Chemicals 1 Acyl, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pm12153579-47-10-27?v=Avanti+Polar
Average 90 stars, based on 1 article reviews
n d m e t h o d s chemicals 1 acyl - by Bioz Stars, 2026-07
90/100 stars
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n- hexanoyl- d-erythro- sphingosine (dcer- c6)  (Matreya LLC)
90
Matreya LLC n- hexanoyl- d-erythro- sphingosine (dcer- c6)
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
N Hexanoyl D Erythro Sphingosine (Dcer C6), supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/10__7554_slash_elife__91708-319-0-7?v=Matreya+LLC
Average 90 stars, based on 1 article reviews
n- hexanoyl- d-erythro- sphingosine (dcer- c6) - by Bioz Stars, 2026-07
90/100 stars
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6-[n-(7-nitro-2,1,3benzoxadiazol-4-yl) amino]-hexanoyl ceramide (c6-nbdcer)  (Matreya LLC)
90
Matreya LLC 6-[n-(7-nitro-2,1,3benzoxadiazol-4-yl) amino]-hexanoyl ceramide (c6-nbdcer)
<t>IL-1β</t> promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 <t>(ERK</t> activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.
6 [N (7 Nitro 2,1,3benzoxadiazol 4 Yl) Amino] Hexanoyl Ceramide (C6 Nbdcer), supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ceramide+c6/pm26377130-51-10-16?v=Matreya+LLC
Average 90 stars, based on 1 article reviews
6-[n-(7-nitro-2,1,3benzoxadiazol-4-yl) amino]-hexanoyl ceramide (c6-nbdcer) - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


IL-1β promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 (ERK activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.

Journal: International Journal of Molecular Medicine

Article Title: Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways

doi: 10.3892/ijmm.2018.3410

Figure Lengend Snippet: IL-1β promoted the expression of ADAMTS-4 and ADAMTS-5 through the activation of MAPK signaling. (A) Of the 3 MAPK isoforms, ERK1/2 and JNK were markedly phosphorylated following treatment with IL-1β. The gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was facilitated by C6 (ERK activator) or Ani (JNK activator). The IL-1β-induced gene expression of (B) ADAMTS-5 and (C) ADAMTS-4 was inhibited by FR (ERK inhibitor) or SP (JNK inhibitor). Data present the means ± standard deviation, n=3. * P<0.05. IL, interleukin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide; C6, Ceramide C6; Ani, Anisomycin; FR, FR180204; SP, SP600125; p, phosphorylated; t, total.

Article Snippet: For the kinase assays, rACs were pretreated with specific inhibitors of ERK (FR180204 at 50 μ M) and JNK (SP600125 at 10 μ M; both from Selleck Chemicals, Houston, TX, USA) and exposed to IL-1β, or treated with the specific activators of ERK (ceramide C6 at 50 μ M; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or JNK (anisomycin at 1 μ M; Selleck Chemicals) alone. rACs treated with DMSO were used as a vehicle control and with IL-1β were used as a positive control.

Techniques: Expressing, Activation Assay, Gene Expression, Standard Deviation

HDAC regulates the osteoarthritis-related genes and protein expression of rACs via ERK1/2 and JNK. (A) IL-1β-induced ERK1/2 phosphorylation was blocked by TSA, and not blocked by PCI; JNK phosphorylation was blocked by TSA and PCI. (B) HDAC inhibitors (TSA or PCI) suppressed HDAC expression at both mRNA and protein level, and prevented the upregulation of osteoarthritis-related genes (col X, COX2, ADAMTS-5 and ADAMTS-4) expression induced by IL-1β. Results are presented with respect to GAPDH expression. (C) siRNAs (targeting HDAC4 or HDAC8) suppressed HDAC expression at both mRNA and protein level, and prevented the upregulation of osteoarthritis-related gene expression induced by IL-1β. Results are expressed with respect to GAPDH expression. Data represent the means ± standard deviation, n=3. * P<0.05. HDAC, histone deacetylase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; IL, interleukin; TSA, Trichostatin A; DMSO, dimethyl sulfoxide; PCI, PCI-34051; col X, type X collagen; COX2, cyclooxygenase; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; siRNA, small interfering RNA; p, phosphorylated; t, total. rACs, rat articular chondrocytes.

Journal: International Journal of Molecular Medicine

Article Title: Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways

doi: 10.3892/ijmm.2018.3410

Figure Lengend Snippet: HDAC regulates the osteoarthritis-related genes and protein expression of rACs via ERK1/2 and JNK. (A) IL-1β-induced ERK1/2 phosphorylation was blocked by TSA, and not blocked by PCI; JNK phosphorylation was blocked by TSA and PCI. (B) HDAC inhibitors (TSA or PCI) suppressed HDAC expression at both mRNA and protein level, and prevented the upregulation of osteoarthritis-related genes (col X, COX2, ADAMTS-5 and ADAMTS-4) expression induced by IL-1β. Results are presented with respect to GAPDH expression. (C) siRNAs (targeting HDAC4 or HDAC8) suppressed HDAC expression at both mRNA and protein level, and prevented the upregulation of osteoarthritis-related gene expression induced by IL-1β. Results are expressed with respect to GAPDH expression. Data represent the means ± standard deviation, n=3. * P<0.05. HDAC, histone deacetylase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; IL, interleukin; TSA, Trichostatin A; DMSO, dimethyl sulfoxide; PCI, PCI-34051; col X, type X collagen; COX2, cyclooxygenase; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; siRNA, small interfering RNA; p, phosphorylated; t, total. rACs, rat articular chondrocytes.

Article Snippet: For the kinase assays, rACs were pretreated with specific inhibitors of ERK (FR180204 at 50 μ M) and JNK (SP600125 at 10 μ M; both from Selleck Chemicals, Houston, TX, USA) and exposed to IL-1β, or treated with the specific activators of ERK (ceramide C6 at 50 μ M; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or JNK (anisomycin at 1 μ M; Selleck Chemicals) alone. rACs treated with DMSO were used as a vehicle control and with IL-1β were used as a positive control.

Techniques: Expressing, Phospho-proteomics, Gene Expression, Standard Deviation, Histone Deacetylase Assay, Small Interfering RNA

Possible signaling pathways associated with ADAMTS, col X, and COX-2 regulation by IL-1β in chondrocytes. ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; col X, type X collagen; COX2, cyclooxygenase; IL, interleukin; HDAC, histone deacetylase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; IL, interleukin; TSA, Trichostatin A; PCI, PCI-34051.

Journal: International Journal of Molecular Medicine

Article Title: Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways

doi: 10.3892/ijmm.2018.3410

Figure Lengend Snippet: Possible signaling pathways associated with ADAMTS, col X, and COX-2 regulation by IL-1β in chondrocytes. ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; col X, type X collagen; COX2, cyclooxygenase; IL, interleukin; HDAC, histone deacetylase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; IL, interleukin; TSA, Trichostatin A; PCI, PCI-34051.

Article Snippet: For the kinase assays, rACs were pretreated with specific inhibitors of ERK (FR180204 at 50 μ M) and JNK (SP600125 at 10 μ M; both from Selleck Chemicals, Houston, TX, USA) and exposed to IL-1β, or treated with the specific activators of ERK (ceramide C6 at 50 μ M; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or JNK (anisomycin at 1 μ M; Selleck Chemicals) alone. rACs treated with DMSO were used as a vehicle control and with IL-1β were used as a positive control.

Techniques: Protein-Protein interactions, Histone Deacetylase Assay