cep55 Search Results


gene exp cep55 hs00216688 m1  (Thermo Fisher)
92
Thermo Fisher gene exp cep55 hs00216688 m1
Gene Exp Cep55 Hs00216688 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/Gene+Exp%2E+CEP55%2C+Hs00216688_m1/us11584967-631-25--1
Average 92 stars, based on 1 article reviews
gene exp cep55 hs00216688 m1 - by Bioz Stars, 2026-09
92/100 stars
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rabbit igg polyclonal anti cep55  (Bioss)
90
Bioss rabbit igg polyclonal anti cep55
Rabbit Igg Polyclonal Anti Cep55, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Polyclonal+Antibody/pmc05502313__jmedgenet___2016___104296supp002-15-2-9
Average 90 stars, based on 1 article reviews
rabbit igg polyclonal anti cep55 - by Bioz Stars, 2026-09
90/100 stars
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cep55 antibody  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc cep55 antibody
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Cep55 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Rabbit+mAb/pmc07849821-129-31-33
Average 93 stars, based on 1 article reviews
cep55 antibody - by Bioz Stars, 2026-09
93/100 stars
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cep55 santa cruz biotechnology  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cep55 santa cruz biotechnology
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Cep55 Santa Cruz Biotechnology, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Antibody/bio_rxiv__2025__10__28__685219-375-34-35
Average 93 stars, based on 1 article reviews
cep55 santa cruz biotechnology - by Bioz Stars, 2026-09
93/100 stars
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antisumo 1  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology antisumo 1
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Antisumo 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+siRNA/pmc01307602-178-35-37
Average 90 stars, based on 1 article reviews
antisumo 1 - by Bioz Stars, 2026-09
90/100 stars
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23891 1 ap  (Proteintech)
93
Proteintech 23891 1 ap
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
23891 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Antibody/pmc11897294-54-14-17
Average 93 stars, based on 1 article reviews
23891 1 ap - by Bioz Stars, 2026-09
93/100 stars
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anti cep55  (Atlas Antibodies)
90
Atlas Antibodies anti cep55
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Anti Cep55, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/Anti-CEP55/pm30607788-43-22-24
Average 90 stars, based on 1 article reviews
anti cep55 - by Bioz Stars, 2026-09
90/100 stars
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guillermo de cárcer marcos malumbres  (Addgene inc)
92
Addgene inc guillermo de cárcer marcos malumbres
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Guillermo De Cárcer Marcos Malumbres, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/pENTR_Mm_CEP55(WT)+(Plasmid+%23136333)/pm39541978-204-60-66
Average 92 stars, based on 1 article reviews
guillermo de cárcer marcos malumbres - by Bioz Stars, 2026-09
92/100 stars
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6a10  (Novus Biologicals)
88
Novus Biologicals 6a10
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
6a10, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Antibody+(6A10)/10__14218_slash_jcth__2021__00036-81-55-56
Average 88 stars, based on 1 article reviews
6a10 - by Bioz Stars, 2026-09
88/100 stars
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cep55  (ProSci Incorporated)
86
ProSci Incorporated cep55
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Cep55, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Antibody/us09409978-682-39-18
Average 86 stars, based on 1 article reviews
cep55 - by Bioz Stars, 2026-09
86/100 stars
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rabbit polyclonal sinobiological  (Sino Biological)
90
Sino Biological rabbit polyclonal sinobiological
Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of <t>CEP55</t> measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.
Rabbit Polyclonal Sinobiological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cep55/CEP55+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pmc09648022__12916_2022_2630_MOESM1_ESM-63-50-52
Average 90 stars, based on 1 article reviews
rabbit polyclonal sinobiological - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of CEP55 measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.

Journal: The Journal of Cell Biology

Article Title: The deubiquitylase USP9X controls ribosomal stalling

doi: 10.1083/jcb.202004211

Figure Lengend Snippet: Characterization of a highly selective USP9X inhibitor. (A) Chemical structure of FT709. (B) In vitro potency of FT709 against USP9X. Activity is monitored by a fluorescence increase following cleavage of a Ub-rhodamine substrate. (C) BxPC3 cell-based potency of FT709 for reduction of CEP55 measured using a MSD ELISA assay. Graphs in B and C show the average of two experiments with error bars indicating the range. (D–F) Cell lysates (D) or intact MCF7 cells (E) were incubated with FT709 (30 min at 25°C for cell extracts, 3 h at 37°C for cells) at the indicated concentrations. Cells were lysed, and extracts were incubated with 0.1 µg HA-UbC2Br probe for 5 min at 37°C, followed by SDS-PAGE analysis. Samples were immunoblotted with USP9X and HA antibodies as indicated. Arrow indicates HA-probe labeled band corresponding to the USP9X~Ub probe adduct. Modification of USP9X with a ubiquitin probe (USP9X~Ub) was lost with increasing concentrations of inhibitor. (F) Quantitation of Western blots shown in D and E. (G and H) HA-based immunoprecipitation of HA-UbC2Br probe–labeled DUBs from cell lysates incubated first with DMSO, 1 or 10 µM FT709, for 1 h at 37°C. Immunoprecipitated proteins were eluted and either analyzed side by side with total lysate samples by immunoblotting (TL, total lysate; EL, eluate) or subjected to mass spectrometry–based quantification in three technical replicates. Differences in DUB-probe binding were quantified for 21 identified DUBs and normalized relative to DMSO control (error bars represent standard deviation of three technical replicates). See for uncropped immunoblots. conc, concentration.

Article Snippet: Samples were analyzed by an MSD ELISA assay (Pacific Biolabs) using a CEP55 antibody (Novux; 1:500 dilution in PBS) captured overnight at 4°C, 30 μl lysates per well, 30 μl of CEP55 antibody (CST; 81693) diluted 1:2,000 in 1% blocker A/PBS and 30 μl per well of a 1:4,000 diluted goat anti-rabbit sulfo-tag, 1% blocker A/PBS.

Techniques: In Vitro, Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Incubation, SDS Page, Labeling, Modification, Ubiquitin Proteomics, Quantitation Assay, Western Blot, Immunoprecipitation, Mass Spectrometry, Binding Assay, Control, Standard Deviation, Concentration Assay