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Image Search Results
Journal: Frontiers in Immunology
Article Title: Enhancing the Detection of Brucella -Specific CD4 + T Cell Responses in Cattle via in vitro Antigenic Expansion and Restimulation
doi: 10.3389/fimmu.2020.01944
Figure Lengend Snippet: RB51-specific proliferation following in vitro antigen stimulation of PBMC from control and RB51-vaccinated animals. Representative histograms showing PBMC CellTrace™ violet staining of total PBMC prior to culture, and following 7 days of culture in media only, with ConA, and RB51 antigen (A) . Frequency of proliferating PBMC from control (open circles) and vaccinated animals (black circles) following a 7-day, in vitro RB51 antigen stimulation, broken down by T cell subset including CD4 + , CD8 + and γδ T cells (B) . Frequency of proliferating CD4 + T cells from control and vaccinated animals left unstimulated or stimulated with RB51 antigen in vitro (C) . Individual animals with mean and standard deviations from the mean shown for all graphs. *indicates P ≤ 0.05, **indicates P ≤ 0.001, and NS indicates no statistical significance.
Article Snippet:
Techniques: In Vitro, Staining