Journal: Bioscience Reports

Article Title: ADSC-Exos containing MALAT1 promotes wound healing by targeting miR-124 through activating Wnt/β-catenin pathway

doi: 10.1042/BSR20192549

Figure Lengend Snippet: ( A ) Cell viability assessed by MTT assay. ( B ) Apoptotic rate of HaCaT and HDF cells exposed with different concentrations of H 2 O 2 examined by flow cytometry assay. ( C ) The percentage of apoptosis cell for HaCaT and HDF cells. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: HaCaT cells were purchased from the American Type Culture Collection (ATCC, Manassas, U.S.A.) and HDF cells were obtained from ScienCell.

Techniques: MTT Assay, Flow Cytometry

Journal: Bioscience Reports

Article Title: ADSC-Exos containing MALAT1 promotes wound healing by targeting miR-124 through activating Wnt/β-catenin pathway

doi: 10.1042/BSR20192549

Figure Lengend Snippet: ( A ) Apoptosis of HaCaT and HDF cells was evaluated by flow cytometry assay. ( B ) The percentage of apoptosis cell for HaCaT and HDF cells. ( C,D ) The capacity of cell migration in HaCaT and HDF cells was analyzed by transwell assay. * P <0.05, **P <0.01, ***P <0.001.

Article Snippet: HaCaT cells were purchased from the American Type Culture Collection (ATCC, Manassas, U.S.A.) and HDF cells were obtained from ScienCell.

Techniques: Flow Cytometry, Migration, Transwell Assay

Journal: Bioscience Reports

Article Title: ADSC-Exos containing MALAT1 promotes wound healing by targeting miR-124 through activating Wnt/β-catenin pathway

doi: 10.1042/BSR20192549

Figure Lengend Snippet: ( A ) The expression level of MALAT1 was measured by qRT-PCR assay. ( B ) Proliferation of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo, H 2 O 2 +ADSC-Exo-shNC or H 2 O 2 +ADSC-Exo-shMALAT1 were evaluated by CCK-8 assay. ( C,D ) Migration of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo, H 2 O 2 +ADSC-Exo-shNC or H 2 O 2 +ADSC-Exo-shMALAT1 were analyzed by Transwell assay. ( E ) The expression level of miR-124 was performed using qRT-PCR analysis. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: HaCaT cells were purchased from the American Type Culture Collection (ATCC, Manassas, U.S.A.) and HDF cells were obtained from ScienCell.

Techniques: Expressing, Quantitative RT-PCR, CCK-8 Assay, Migration, Transwell Assay

Journal: Bioscience Reports

Article Title: ADSC-Exos containing MALAT1 promotes wound healing by targeting miR-124 through activating Wnt/β-catenin pathway

doi: 10.1042/BSR20192549

Figure Lengend Snippet: ( A ) The expression level of miR-124 was detected by qRT-PCR after transfected with anti-miR-124. ( B ) CCK-8 assay was performed to assess the cell proliferation of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo, H 2 O 2 +ADSC-Exo-shNC, H 2 O 2 +ADSC-Exo-shMALAT1, H 2 O 2 +ADSC-Exo-shMALAT1+anti-miR-124. ( C ) Flow cytometry was subjected to evaluate cell apoptosis of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo, H 2 O 2 +ADSC-Exo-shNC, H 2 O 2 +ADSC-Exo-shMALAT1, H 2 O 2 +ADSC-Exo-shMALAT1+anti-miR-124. ( D ) Migration of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo, H 2 O 2 +ADSC-Exo-shNC, H 2 O 2 +ADSC-Exo-shMALAT1, H 2 O 2 +ADSC-Exo-shMALAT1+anti-miR-124 were analyzed by the scratch wound healing assay. ( E ) The expression of Wnt/β-catenin signals were determined by western blot. ( F ) Cell proliferation of HaCaT and HDF cells treated with FH535 were evaluated by CCK-8 assay. ( G ) Wnt/β-catenin signal pathway of HaCaT and HDF cells treated with H 2 O 2 , H 2 O 2 +ADSC-Exo or H 2 O 2 +ADSC-Exo+FH535 were monitored by western blot. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: HaCaT cells were purchased from the American Type Culture Collection (ATCC, Manassas, U.S.A.) and HDF cells were obtained from ScienCell.

Techniques: Expressing, Quantitative RT-PCR, Transfection, CCK-8 Assay, Flow Cytometry, Migration, Wound Healing Assay, Western Blot

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

doi: 10.3389/fbioe.2024.1385730

Figure Lengend Snippet: 5-Fluorouracil (5-FU) with cytotoxic and detachment-enhancing effect on the colorectal cancer cell (CRC) line HT-29 but without effect on human dermal fibroblast (HDF) cell viability and proliferation. (A) 5-FU effects via CAN spectroscopy on HT-29 cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated HT-29 grew on the CMOS MEA and the cell-covered area rose (A-i) . After 5-FU treatment, the detection positive area declined after 72 h of cultivation (A-ii) . Statistics (A-i,A-ii) : one-way ANOVA. Statistical difference of the cell-covered area between untreated and treated HT-29 (A-iii) . Statistics (A-iii) : two-way ANOVA. (B) 5-FU effects via CAN spectroscopy on HDF cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated and treated HDFs showed a stagnant CMOS MEA area where cells are adhered to (B-i,B-ii) . Statistics (B-i,B-ii) : one-way ANOVA. CMOS MEA area covered by HDFs (B-iii) . Statistics (B-iii) : two-way ANOVA. (C) 5-FU effects on HT-29 cells via CCK-8 assay. Untreated HT-29 cells’ optical density (OD) increased during 72 h cultivation time (C-i) , while 5-FU treated cells’ OD declined (C-ii) . (D) 5-FU effects on HDF cells via CCK-8 assay. For HDFs, the OD stays constant without (D-i) and with 5-FU treatment (D-ii) . Statistics: one-way ANOVA. (C-iii,D-iii) compared cell viability of treated cells with untreated cells as control group. Treated cells showed less cell viability for both cell types [HT-29 (C-iii) and HDF (D-iii) ]. Statistics: two-way ANOVA. (E) Cell viability from CASY recordings of treated and untreated HT-29 in the supernatant and on the chip (E-i) . Same for HDFs (E-ii) . Two-way ANOVA. Statistical significance is indicated by asterisks (n HT-29 = 4, n HDF = 4, ns, not significant. *, p ≤ 0.05. **, p ≤ 0.01. ***, p ≤ 0.001. ****, p ≤ 0.0001).

Article Snippet: The colorectal cancer (CRC) cell line HT-29 (ATCC, RRID: CVCL_0320) and the human dermal fibroblast cell line HDF (PELOBiotech GmbH, RRID: CVCL_DP66) were cultivated at 37°C in a 5% CO 2 atmosphere.

Techniques: Spectroscopy, CCK-8 Assay, Control

Journal: Molecular Cancer

Article Title: Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma

doi: 10.1186/1476-4598-13-185

Figure Lengend Snippet: The AKT/mTOR pathway is constitutively activated in Asra-EPS and VAESBJ cell lines. A) Expression of proteins related to the AKT/mTOR pathway in Asra-EPS, VAESBJ, and HDF cells. B) Expression of p-AKT in the absence or presence of serum in Asra-EPS and VAESBJ cells. Cells were seeded in normal growth medium, grown over night, and incubated in the absence or presence of serum for 12 hours.

Article Snippet: HDF cells were purchased from Kurabo.

Techniques: Expressing, Incubation

Journal: Molecular Cancer

Article Title: Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma

doi: 10.1186/1476-4598-13-185

Figure Lengend Snippet: RAD001 inhibits EpS cell growth but increases AKT activation in vitro . A) Sensitivities of Asra-EPS, VAESBJ, and HDF cells to RAD001. Cells were exposed to various concentrations of RAD001 for 72 hours. Cell viability was determined by WST-1 assay. Points, mean; bars, SD. B) PI staining fluorescence-activated cell sorting analyses of the DNA contents of Asra-EPS and VAESBJ cells in response to RAD001. Cells were treated with 0.25–4 nM of RAD001 or vehicle for 24 hours. C) Effects of RAD001 on phosphorylation of S6RP and AKT in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–4 nM of RAD001 or vehicle for 6 hours and with 1 nM RAD001 for 1–24 hours.

Article Snippet: HDF cells were purchased from Kurabo.

Techniques: Activation Assay, In Vitro, WST-1 Assay, Staining, Fluorescence, FACS, Phospho-proteomics

Journal: Molecular Cancer

Article Title: Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma

doi: 10.1186/1476-4598-13-185

Figure Lengend Snippet: c-MET is highly activated through an autocrine mechanism in EpS, and c-MET activation affects EpS cell growth. A) Expression of phosphorylated RTKs in Asra-EPS and VAESBJ cells. B) Protein expression of c-MET and p-MET in Asra-EPS, VAESBJ, SYO-1, and HDF cells. C) HGF levels in cell-conditioned media and HGF serum concentrations in mice bearing xenograft tumors. Columns, mean; bars, SD. D) Expression of c-MET and p-MET in Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. E) Proliferation of Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. Cells transfected with siRNAs were cultured for 96 hours. Cell viability was determined by WST-1 assay every 24 hours. Points, mean; bars, SD. **, p < 0.01, compared with control. F) Colony formation of Asra-EPS and VAESBJ cells transfected with anti-c-MET siRNAs or a control siRNA. Columns, mean; bars, SD. **, p < 0.01, compared with control.

Article Snippet: HDF cells were purchased from Kurabo.

Techniques: Activation Assay, Expressing, Transfection, Control, Cell Culture, WST-1 Assay

Journal: Molecular Cancer

Article Title: Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma

doi: 10.1186/1476-4598-13-185

Figure Lengend Snippet: INC280 inhibits EpS cell growth in vitro , but Asra-EPS and VAESBJ cells differ from each other with regard to dependency on c-MET signaling. A) Sensitivities of Asra-EPS, VAESBJ, SYO-1, and HDF cells to INC280. Cells were exposed to different concentrations of INC280 for 72 hours. Cell viability was determined by WST-1 assay. Points, mean; bars, SD. B) PI staining fluorescence-activated cell sorting analyses of the DNA contents of Asra-EPS and VAESBJ cells in response to INC280. Asra-EPS and VAESBJ cells were incubated with 0.25–4 nM and 0.25–16 nM of INC280 for 24 hours, respectively. C) Effects of INC280 on caspase-3 cleavage in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–64 nM of INC280 or vehicle for 24 hours. Staurosporine was used as a positive control. D) Effects of INC280 on phosphorylation of c-MET and its downstream effectors in Asra-EPS and VAESBJ cells. Cells were treated with 0.25–4 nM of INC280 or vehicle for 1 hour.

Article Snippet: HDF cells were purchased from Kurabo.

Techniques: In Vitro, WST-1 Assay, Staining, Fluorescence, FACS, Incubation, Positive Control, Phospho-proteomics