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Image Search Results
Journal: Frontiers in Immunology
Article Title: Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway
doi: 10.3389/fimmu.2025.1577234
Figure Lengend Snippet: THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system (IVIS) images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Article Snippet: Fourteen days post-surgery, in vivo 3D optical imaging was performed using the
Techniques: In Vivo Imaging, Real-time Polymerase Chain Reaction, Immunofluorescence, Micro-CT, Staining, Immunohistochemical staining