cellometer Search Results


96
Revvity ivis system
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Ivis System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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96
Revvity victor nivo multimode plate reader
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Victor Nivo Multimode Plate Reader, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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96
Revvity opera phenix
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Opera Phenix, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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96
Revvity cellometer auto t4 plus
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Cellometer Auto T4 Plus, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Revvity cellometertm mini automated cell counter
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Cellometertm Mini Automated Cell Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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96
Revvity cellometer k2 fluorescent cell counter
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Cellometer K2 Fluorescent Cell Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Revvity cellometer k2 image cytometer
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Cellometer K2 Image Cytometer, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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94
Revvity th115 pcr5h
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Th115 Pcr5h, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Revvity 1211 rackbeta liquid scintillation counter
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
1211 Rackbeta Liquid Scintillation Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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96
Revvity cell viability
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Cell Viability, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Revvity spectrometer
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Spectrometer, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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94
Revvity phenovue cell painting kit
(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by <t>IVIS</t> imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
Phenovue Cell Painting Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by IVIS imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: bioRxiv

Article Title: Coordinated Tuning of Ionizable Lipids and Formulation Redirects mRNA Vaccines Toward Lymphoid-Specific CD4 + T Cell Immunity

doi: 10.64898/2026.04.16.719092

Figure Lengend Snippet: (a) Experimental scheme for in vivo screening. C57BL/6N mice were intramuscularly administered fLuc mRNA-loaded LNPs (0.05 mg/kg) and bioluminescence was assessed at 6 h post-injection by IVIS imaging. (b) Representative in vivo IVIS bioluminescence images of nine LNP formulations. (c) Quantification of bioluminescence signals at the injection site. (d) Quantification of bioluminescence signals in the liver. (e) Injection site-to-liver bioluminescence ratio (n = 2 per group). N4Y and N4Z were selected as lead candidates based on high injection site expression and minimal hepatic off-target signal. (f, g) Time-dependent serum levels of MCP-1 (f) and IL-6 (g) following intramuscular administration of N4Y- or N4Z-based LNPs encapsulating SARS-CoV-2 spike mRNA (0.5 mg/kg) in BALB/c mice (n = 4 per group). Statistical significance was determined by unpaired two-tailed Student’s t-test at each time point. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Luciferase expression was assessed by whole-body bioluminescence imaging and ex vivo imaging of harvested organs using an IVIS system (PerkinElmer).

Techniques: In Vivo, Injection, Imaging, Expressing, Two Tailed Test

(a) Schematic comparison of lipid compositions of N4Z and optimized N4Z (N4Z-opt) formulations. (b) mRNA encapsulation efficiency (n = 3). (c) Hydrodynamic diameter (n = 3). (d) Polydispersity index (PDI) (n = 3). (e) Representative flow cytometry contour plots showing GFP expression in RAW 264.7 macrophages following delivery of PBS, N4Z, and N4Z-opt. (f) Mean fluorescence intensity (MFI) of GFP expression in RAW 264.7 macrophages (n = 3). (g) Representative in vivo IVIS bioluminescence images following intramuscular administration of fLuc mRNA-loaded N4Z or N4Z-opt LNPs. (h) Injection site-to-body bioluminescence ratio (n = 3). (i) Representative ex vivo organ images showing bioluminescence in liver, spleen, and lymph node. (j) Spleen-to-liver bioluminescence ratio (n = 3). (k) Lymph node-to-liver bioluminescence ratio (n = 3). Statistical significance for (b–d) was determined by unpaired two-tailed Student’s t -test. Statistical significance for (f, h, j, k) was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: Coordinated Tuning of Ionizable Lipids and Formulation Redirects mRNA Vaccines Toward Lymphoid-Specific CD4 + T Cell Immunity

doi: 10.64898/2026.04.16.719092

Figure Lengend Snippet: (a) Schematic comparison of lipid compositions of N4Z and optimized N4Z (N4Z-opt) formulations. (b) mRNA encapsulation efficiency (n = 3). (c) Hydrodynamic diameter (n = 3). (d) Polydispersity index (PDI) (n = 3). (e) Representative flow cytometry contour plots showing GFP expression in RAW 264.7 macrophages following delivery of PBS, N4Z, and N4Z-opt. (f) Mean fluorescence intensity (MFI) of GFP expression in RAW 264.7 macrophages (n = 3). (g) Representative in vivo IVIS bioluminescence images following intramuscular administration of fLuc mRNA-loaded N4Z or N4Z-opt LNPs. (h) Injection site-to-body bioluminescence ratio (n = 3). (i) Representative ex vivo organ images showing bioluminescence in liver, spleen, and lymph node. (j) Spleen-to-liver bioluminescence ratio (n = 3). (k) Lymph node-to-liver bioluminescence ratio (n = 3). Statistical significance for (b–d) was determined by unpaired two-tailed Student’s t -test. Statistical significance for (f, h, j, k) was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Luciferase expression was assessed by whole-body bioluminescence imaging and ex vivo imaging of harvested organs using an IVIS system (PerkinElmer).

Techniques: Comparison, Encapsulation, Flow Cytometry, Expressing, Fluorescence, In Vivo, Injection, Ex Vivo, Two Tailed Test