Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A) Human leukocytes/carcinoma cells are dispersed on a cell microarray chip, followed by 15 minutes' standing to allow the cells to settle down into the microchambers, and are then stained with fluorescence-labeled antibodies against carcinoma cells. (B) Fluorescence-positive CTCs are detected by using a microarray scanner with a confocal fluorescence laser. (C) The target CTCs are analyzed quantitatively at the single-cell level.

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray, Staining, Fluorescence, Labeling

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A) Photo of an actual cell microarray chip. (B, C) SEM images of a cell microarray chip. The cell microarray chip comprises 20,944 microchambers in a plastic slide on a glass slide. The cell microarray chip has 112 (14×8) clusters, each having 187 microchambers. (D) Each microchamber is 105 µm in upper diameter, 50 µm in depth, and has the shape of a frustum with a 68-µm diameter flat bottom for the accommodation of leukocytes as a monolayer.

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A, B) Photographic light microscopic images of T lymphoblastoid leukemia cells incubated on a cell microarray chip before (A) and after (B) washing of the chip surface. (C–F) Photos of microchamber appearance after washing when T lymphoblastoid leukemia suspensions of 2.5×10 6 (C), 5.0×10 6 (D), 7.5×10 6 (E) or 1.0×10 7 (F) cells/ml were applied to the microarray chip. Concentrations of 7.5×10 6 cells/ml of T lymphoblastoid leukemia and above afforded tight confinement and formation of a monolayer in the microchambers. (Bar: 20 µm).

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Incubation, Microarray

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A, B) Photographic light microscopic images of carcinoma cells on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) Carcinoma cells showed tight confinement and had formed a monolayer in the microchamber when a concentration of 7.5×10 6 cells/ml was used. (Bar: 20 µm).

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray, Concentration Assay

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A, B) Photographic light microscopic images of leukocytes on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) The leukocytes showed tight confinement and had formed a monolayer in the microchamber. (Bar: 20 µm).

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A–I) Scanned images of leukocytes/carcinoma cells on a cell microarray chip obtained with the microarray scanner. (A) Negative control (no carcinoma cells). (B, D, G) Carcinoma cells (0.01, 0.001, and 0.0001%) were scanned in 3, 9, and 64 clusters, respectively, on the cell microarray chip. (C, E, F, H, I) Magnified views of the boxed regions. Color scale represents the intensity of fluorescent emission.

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray, Negative Control

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A) Scanned images of cells on a cell microarray chip, obtained with the microarray scanner. The cells were immunostained with PE-labeled anti-cytokeratin monoclonal antibody. (B) Magnified view of the boxed region. Color scale represents the intensity of fluorescent emission.

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray, Labeling

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: Analysis of spiked carcinoma cells in human whole blood on a cell microarray chip.

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Microarray

Journal: PLoS ONE

Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

doi: 10.1371/journal.pone.0032370

Figure Lengend Snippet: (A–L) Cultured T lymphoblastoid leukemia (A–F) and leukocytes isolated from whole blood (F–L) were stained with PE-labeled anti-cytokeratin monoclonal antibody (A, G) and APC-labeled anti-EpCAM monoclonal antibody (C, I). (E, K) Merged images identify doubly-positive carcinoma cells in each panel. Magnified views of the boxed regions (B, D, F, H, J, L). Scatter-plot analysis of 3 cluster areas representing 561 microchambers in a cell microarray chip .

Article Snippet: As shown in , the cell microarray chip comprised 20,944 microchambers (105-μm upper diameter, 68-μm lower diameter, 50-μm depth, and spacing as indicated) and was made from polystyrene by the Lithographic Galvanoformung Abformung process by Starlight Co. Ltd. (Osaka, Japan) .

Techniques: Cell Culture, Isolation, Staining, Labeling, Microarray