cell lines a673 Search Results


a673  (CLS Cell Lines Service GmbH)
93
CLS Cell Lines Service GmbH a673
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
A673, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/A673+Cells/pmc12286862-336-21-26
Average 93 stars, based on 1 article reviews
a673 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
a-673 cell lines complete growth medium  (Innovative Research Inc)
N/A
A-673 Cell Lines Complete Growth Medium is a cell lines complete growth medium from Innovative Research, supplied as a ready-to-use liquid. More Details: Formulation: DMEM + 10% FBS + 1% P/S Bacterial detection: Negative Fungal
   Buy from Supplier
a673 cell line  (DS Pharma Biomedical)
90
DS Pharma Biomedical a673 cell line
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
A673 Cell Line, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/a673+cell+line/pmc09184620-236-5-11
Average 90 stars, based on 1 article reviews
a673 cell line - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
cell lines a673, tc-32, sk-es-1, and rd-es  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications cell lines a673, tc-32, sk-es-1, and rd-es
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
Cell Lines A673, Tc 32, Sk Es 1, And Rd Es, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/cell+lines+a673++tc+32++sk+es+1++and+rd+es/10__1158_slash_1541___7786__mcr___11___0390-64-7-15
Average 90 stars, based on 1 article reviews
cell lines a673, tc-32, sk-es-1, and rd-es - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
a673 cell line derivates with stable shrna silencing of ews–fli1 (ews–fli1– off)  (Huntsman International LLC)
90
Huntsman International LLC a673 cell line derivates with stable shrna silencing of ews–fli1 (ews–fli1– off)
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
A673 Cell Line Derivates With Stable Shrna Silencing Of Ews–Fli1 (Ews–Fli1– Off), supplied by Huntsman International LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/a673+cell+line+model/pmc05423155-84-9-26
Average 90 stars, based on 1 article reviews
a673 cell line derivates with stable shrna silencing of ews–fli1 (ews–fli1– off) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
human ewing sarcoma cell line a673  (JCRB Cell Bank)
90
JCRB Cell Bank human ewing sarcoma cell line a673
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
Human Ewing Sarcoma Cell Line A673, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/human+ewing+sarcoma+cell+line+a673/pmc10826185-226-7-54
Average 90 stars, based on 1 article reviews
human ewing sarcoma cell line a673 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
a673 cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures a673 cell line
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
A673 Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/a673+cell+line/pm36194562-41-1-8
Average 90 stars, based on 1 article reviews
a673 cell line - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
a-673  (ATCC)
96
ATCC a-673
a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of <t>A673</t> cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.
A 673, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+a673/A-673/custom%40crl-1598%4028521819
Average 96 stars, based on 1 article reviews
a-673 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
a 673 cell line  (Elabscience)
N/A
   Buy from Supplier

Image Search Results


a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of A673 cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.

Journal: Nature

Article Title: Probing condensate microenvironments with a micropeptide killswitch

doi: 10.1038/s41586-025-09141-5

Figure Lengend Snippet: a . Live cell fluorescence microscopy images of U2OS cells expressing ectopic mEGFP-fsHMGB1 variants and RFP-FIB1. The cell nucleus is highlighted with a dashed white line contour. Scale bar: 5 µm. b . (left) Model of fsHMGB1 and sequences of the KS variants within the tested mEGFP-HMGB1 proteins. (right) Quantification of circularity of nucleoli in cells expressing ectopic mEGFP-HMGB1 KS variants. P -values from Dunnett’s multiple comparison test versus fsHMGB1-full length after one-way ANOVA. P ( Δkillswitch ) = <0.0001, P ( F-to-E&D ) = < 0.0001, P ( F-to-A ) = 0.0009, P ( F-to-G ) = < 0.0001, P ( 0F ) = 0.0972, P ( F12A ) = 0.0003, P ( F13A ) = > 0.9999, P ( ΔKS-3F ) = < 0.0001, P ( 11G3F3G ) = < 0.0001, P ( C16-to-A ) = 0.0346, P ( M-to-E&D ) = < 0.0001. ****: P < 10 −4 , ***: P < 10 −3 , * : P < 0.05. c . FRAP of the indicated mEGFP-HMGB1 variants. The quantification of the corresponding mobile/immobile fraction ratios are shown in Fig. . Data are mean ± s.d. d . Cell viability of U2OS cell expressing mEGFP-HMGB1 proteins. Data are mean ± s.d. P -values from one-way ANOVA Dunnett’s multiple comparison test versus fsHMGB1-full length. (Left): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.5987, P ( F-to-D&E ) = 0.0320, P ( M-to-D&E ) = < 0.0001, P ( C-to-A ) = < 0.0001; (Middle): P ( Full length ) = <0.0001, P ( Δkillswitch ) = 0.9975, P ( F-to-D&E ) = 0.8919, P ( F-to-A ) = 0.9967, P ( F-to-G ) = < 0.5244. (Right): P ( Full length ) = 0.0046, P ( Δkillswitch ) = 0.2873, P ( F-to-G ) = 0.4834, P ( ΔKS-3F ) = 0.4818, P ( 0F ) = 0.6900, P ( 11G3F3G ) = 0.4578. n = three (except two for F-to-D&E from the middle plot and ΔKS-3F from the right plot) biologically independent experiments. e . Representative live cell fluorescence microscopy images of A673 cells expressing EGFP-NPM1-WT and -KS variants 24 h after doxycycline induction. The cell nucleus is highlighted with a dashed white line contour. The experiments were repeated independently twice with similar results. Scale bar: 5 µm. f . Quantification of the mobile and immobile fractions of EGFP-NPM1 proteins. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA. P ( KS ) = < 0.0001, P ( KS_F-to-G ) = 0.76, P ( KS_F-to-A ) = 0.03, P ( KS_0F ) = 1.00. g . Mean GFP fluorescence of the bleached area. Data are mean ± s.d. n = 15 cells for all samples from two biologically independent experiments. P -values from Dunnett’s multiple comparisons test versus EGFP-NPM1-WT after one-way ANOVA.

Article Snippet: U2-OS (ATCC, HTB-96) HEK293T (ATCC, CRL-3216), HCT-116 (ATCC, CCL-247), MCF7 (ATCC, HTB-22), C2C12 (ATCC, CRL-1772), Lenti-X 293T (Takara Bio, 632180) and A673 (gifted by H. Kovar; CLS, 300454) cell lines were cultured in DMEM GlutaMAX (Gibco, 31966047).

Techniques: Fluorescence, Microscopy, Expressing, Comparison