Journal: Med (New York, N.y.)

Article Title: Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset

doi: 10.1016/j.medj.2021.04.003

Figure Lengend Snippet:

Article Snippet: No-GFP-CD16.NK-92 cell line (High affinity 176V) , ATCC , PTA-6967.

Techniques: Virus, Recombinant, Staining, Luminex, Neutralization, Generated, Software

Journal: Scientific Reports

Article Title: Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model

doi: 10.1038/s41598-019-48092-6

Figure Lengend Snippet: ( A ) Human corneal epithelial cells (HCEC) were subjected to in vitro scratch assay. Representative images from scratch wound healing assay of HCEC showing time course of corneal re-epithelialization (abrasion 1 mm) in vitro model at different time intervals (0, 6, 12, 18 & 24 hours) after post wounding. Scratch wounds were made in confluent cultures of corneal epithelial cells. The red lines define the area lacking cells where as arrows indicating the movement of cells towards closing the wound. The images were analyzed by Image J software (National Institutes of Health [NIH], Bethesda, MD, USA) with Scale bar = 100 µm. Images were captured at 4X magnification using camera-equipped inverted microscope (Olympus Onvented, DSR-012). ( B ) In vitro wound healing of migrating corneal epithelia in confluent monolayer of HCEC showing linear phase of wound healing at time intervals 6, 12, 18 and 24 hours while wound was closed at 24 hours of post wounding. Cellular migration was calculated using one way ANOVA by GraphPad (7.0) with significance of p < 0.001. Each value is representing three individual experiments, error bars indicates SDM.

Article Snippet: The Human corneal epithelial cell (HCEC), were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: In Vitro, Wound Healing Assay, Software, Inverted Microscopy, Migration

Journal: Scientific Reports

Article Title: Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model

doi: 10.1038/s41598-019-48092-6

Figure Lengend Snippet: Western Blot analysis detected expression of cdk10 and ETS2 in HCEC: ( A) The expression level of cdk10 and ETS2 at active hours of migration (18 hours) related to non-migrating sample with beta- Actin as loading control. lane 1 NM, non-migrating; lane 2, migrating at 18 hrs. (B) Quantification and intensity measurement of relative protein expression were analyzed by Quantity One software (Bio-Rad, USA). Histograms are generated using GraphPad Prism software (7.04).Values are expressed as ± SD, significance (P < 0.005) was calculated using one way ANOVA test statistically.

Article Snippet: The Human corneal epithelial cell (HCEC), were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Western Blot, Expressing, Migration, Control, Software, Generated

Journal: Journal of Hematology & Oncology

Article Title: Challenges and advances in clinical applications of mesenchymal stromal cells

doi: 10.1186/s13045-021-01037-x

Figure Lengend Snippet: The tests of modified MSCs using CRISPR-Cas9 technology

Article Snippet: Immortalized human bone marrow MSC cell line (ATCC PCS-500–041) , PUMILIO2 (PUM2) , Depletion of PUM2 blocks MSC adipogenesis and enhances osteogenesis. PUM2 works as a negative regulator on the 3′ UTRs of JAK2 and RUNX2 via direct binding. CRISPR/CAS9-mediated gene silencing of Pum2 inhibited lipid accumulation and excessive bone formation , [ ] .

Techniques: Modification, CRISPR, Functional Assay, Synthesized, In Vitro, Transfection, Expressing, Transplantation Assay, Inhibition, Binding Assay

Journal: Journal of neurochemistry

Article Title: cDNA cloning and molecular characterization of human brain metalloprotease MP100: a beta-secretase candidate?

doi: 10.1046/j.1471-4159.1999.0721215.x

Figure Lengend Snippet: FIG. 2. Expression of active hMP100L. A: The long form of recombinant human MP100 showed increased MP100 activity compared with vector alone-transfected HEK cells using 150 mg of cell extract and b-APP peptide-pNA digestion. The activity was inhibited by the metalloprotease inhibitor o-phenanthroline (3 mM). B: MP100 western blotting confirmed strong overex- pression of recombinant MP100L (arrow).

Article Snippet: Culture and transfection of the HEK293 cell line (CRL 1573; ATCC) were done as described by Malherbe et al. (1994).

Techniques: Expressing, Recombinant, Activity Assay, Plasmid Preparation, Transfection, Western Blot

Journal: Journal of neurochemistry

Article Title: cDNA cloning and molecular characterization of human brain metalloprotease MP100: a beta-secretase candidate?

doi: 10.1046/j.1471-4159.1999.0721215.x

Figure Lengend Snippet: FIG. 4. Double MP100/b-APP immunofluorescence by confocal microscopy. A: Stable HEK-b-APP695 cells transiently coex- pressing hMP100L show colocalization of MP100 (Texas red- labeled) and b-APP (FITC-labeled) at certain locations, although considerable amounts of both proteins are also differently ex- pressed. The colocalized areas are visualized in white (as de- scribed in Materials and Methods) and marked by arrows. B: Human neuroblastoma IMR32 cells displaying endogenous MP100 (Texas red-labeled) and b-APP (FITC-labeled) show sim- ilar partial colocalization of the two proteins, whereas fractions of both proteins are noncolocalized.

Article Snippet: Culture and transfection of the HEK293 cell line (CRL 1573; ATCC) were done as described by Malherbe et al. (1994).

Techniques: Confocal Microscopy, Labeling

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Enhancement of gene transfer to human myeloid cells by adenovirus-fiber complexes.

doi: 10.1038/sj.mt.6300048

Figure Lengend Snippet: Figure 2 37FK enhances Ad uptake in myeloid, but not lymphoid cells. (a, b) Human monocytes are preferentially susceptible to 37FK-enhanced Ad transduction. MACS isolation was used to negatively and positively select monocytes and lymphocytes, respectively, from human PBMCs. Cells were incubated with buffer (white bars), or 50 mg/ml of 16FK (gray bars) or 37FK (black bars) and 1,000 p/cell of Ad5.F5 or 100 p/cell of Ad5.F16 for 1 h on ice, and then assayed for transduction as described in Figure 1. Contaminating cell types in each subpopulation were excluded from the analyses by appropriate gating based on forward and side scatter parameters. (c, d) 37FK does not enhance Ad transduction of human B- and T-cell lines. The HSB-2 human T lymphoblastoid and JR2 human B lymphoblastoid cell lines were incubated with buffer or FKs and Ads and assayed for transduction as described above. All data shown are mean percentages of transduced cells7SD of triplicate samples of one experiment and are representative of at least three experiments similarly performed.

Article Snippet: The U-937 human histiocytic lymphoma, and HSB-2 human T lymphoblastoid cell lines were from the American Type Culture Collection (Manassas, VA), and maintained in Rosewell Park Memorial Institute medium (RPMI) 1640 supplemented with 10% fetal calf serum (Omega Scientific, Tarzana, CA), 10 mM N-2-hydroxylpiperazine-N0-2-ethanesulfonic acid (pH 7.55), 4 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin.

Techniques: Transduction, Isolation, Incubation