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Image Search Results
Journal: International journal of cardiology
Article Title: Enhanced external counterpulsation inhibits endothelial apoptosis via modulation of BIRC2 and Apaf-1 genes in porcine hypercholesterolemia.
doi: 10.1016/j.ijcard.2013.11.033
Figure Lengend Snippet: Fig. 2. Isolation and identification of vascular endothelial cells from the aortic endothelium with collagenase. Representative photomicrographs (×400) of isolated cells treated histocytochemically with CD31 antibody and incubated with DiI-Ac-LDL. Cells with cytomembrane and cytoplasmic staining of amber color indicate vascular endothelial cells (black arrow), and cells with red fluorescence were identified as viable vascular endothelial cells (white arrow), which consisted of the majority of total treated cells, indicating a successful en- dothelial cell isolation from aortic endothelium.
Article Snippet: Then
Techniques: Isolation, Incubation, Staining, Cell Isolation
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: List of up-regulated proteins during Ang II infusion.
Article Snippet: The primary
Techniques: Ubiquitin Proteomics, Migration, Membrane
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: Immunohistochemical verification of DEPs. ( A ) Immunohistochemistry staining of up-regulated proteins, such as lysine-specific histone demethylase 1A (KDM1A), serine/threonine-protein kinase N1 (PKN1), C-terminal-binding protein 1 (CtBP1), transmembrane protein 41B (TMEM41B), myeloblastin (PRTN3), CD166 antigen (ALCAM), and GRB10-interacting GYF protein 2 (GIGYF2). Positive staining was indicated by brown coloration, and nuclei were stained with hematoxylin in blue. ( B ) Immunohistochemistry staining of down-regulated proteins, such as CCN family member 1 (CCN1), muscleblind-like protein 1 (MBNL1), H/ACA ribonucleoprotein complex subunit 2 (NHP2), retinol dehydrogenase 10 (RDH10), T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4), and Translin (TSN). Positive cells are indicated by brown coloration.
Article Snippet: The primary
Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Binding Assay
Journal: Biomedicines
Article Title: Comparative Proteomic Analysis of tPVAT during Ang II Infusion
doi: 10.3390/biomedicines9121820
Figure Lengend Snippet: DEPs with high connectivity degree in PPI analysis between saline and Ang II infusion group.
Article Snippet: The primary
Techniques: Saline, Ubiquitin Proteomics
Journal: PLoS ONE
Article Title: Necl-4/SynCAM-4 Is Expressed in Myelinating Oligodendrocytes but Not Required for Axonal Myelination
doi: 10.1371/journal.pone.0064264
Figure Lengend Snippet: A. Western immuneblotting of brain tissues (from P10 wild-type and Necl-4 homozygous mice) with anti-Necl-1, anti-Necl-2 and anti-Necl-3 antibodies. β-actin was the internal control. B. Statistical analysis on the relative expression level of Necl1, Necl2 and Necl3 with Student's t-test (n = 3. Necl1, p = 0.70. Necl2, p = 0.72. Necl3, p = 0.69). Error bar, standard deviation.
Article Snippet: 30 mg protein from control and mutant tissues was loaded for SDS-PAGE electrophoresis and subsequently detected with anti-Necl-1 (developed in Peking Union Medical University),
Techniques: Western Blot, Expressing, Standard Deviation