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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: The effect of human immunodeficiency virus-1 on monocyte-derived dendritic cell maturation and function
doi: 10.1111/j.1365-2249.2012.04628.x
Figure Lengend Snippet: Human immunodeficiency virus (HIV)-1 infection of monocyte-derived dendritic cells (MDDC) inhibits antigen presentation to autologous CD8+ T cells. HIV-1CS204-infected immature MDDC (iMDDC) were pulsed with a cytomegalovirus, Epstein–Barr and influenza viruses (CEF) peptide pool and then co-cultured with autologous 5 mM CellTrace™ carboxyfluorescein succinimidyl ester (CFSE)-stained peripheral blood mononuclear cells (PBMC) at a ratio of 1:10 for 7 days. While preincubation of uninfected iMDDC induced the proliferation of autologous monocyte depleted PBMC, HIV-1CS204 infection of iMDDCs impaired the presentation of a CEF peptide pool as measured by CFSE dilution. Results are expressed as means ± standard deviation. *P = 0·02 by paired t-test (n = 6).
Article Snippet: MDDCs, differentiated and infected as above, were pulsed for 3 h with 3 μg/ml of a
Techniques: Virus, Infection, Derivative Assay, Immunopeptidomics, Cell Culture, Staining, Standard Deviation