Journal: The Journal of International Medical Research

Article Title: Prognostic significance of CEA, Ki67 and p53 in pseudomyxoma peritonei of appendiceal origin

doi: 10.1177/03000605211022297

Figure Lengend Snippet: Univariate analysis of overall survival of patients ( n = 141) with pseudomyxoma peritonei.

Article Snippet: Immunohistochemical staining was performed using primary mouse monoclonal antibodies against Ki67 (clone UMAB107 ready to use; OriGene China, Beijing, China), p53 (clone DO7 ready to use; OriGene China) and CEA (clone COL1 ready to use; OriGene China) that were processed in automated staining equipment (INTELLIPATH FLX®; Biocare Medical, Pacheco, CA, USA).

Techniques:

Journal: The Journal of International Medical Research

Article Title: Prognostic significance of CEA, Ki67 and p53 in pseudomyxoma peritonei of appendiceal origin

doi: 10.1177/03000605211022297

Figure Lengend Snippet: The difference in clinicopathological characteristics between patients ( n = 141) with pseudomyxoma peritonei stratified according to Ki67 and p53 immunostaining levels.

Article Snippet: Immunohistochemical staining was performed using primary mouse monoclonal antibodies against Ki67 (clone UMAB107 ready to use; OriGene China, Beijing, China), p53 (clone DO7 ready to use; OriGene China) and CEA (clone COL1 ready to use; OriGene China) that were processed in automated staining equipment (INTELLIPATH FLX®; Biocare Medical, Pacheco, CA, USA).

Techniques: Immunostaining

Journal: ACS applied bio materials

Article Title: Targeted Biodegradable Near-Infrared Fluorescent Nanoparticles for Colorectal Cancer Imaging.

doi: 10.1021/acsabm.4c00072

Figure Lengend Snippet: Figure 6. Target specific accumulation of CEA-FSNs in PIRC rats was confirmed by macroscopic and microscopic imaging of excised tissues. (A) Ab-FSNs were topically applied to polyps (yellow arrows) that were observed by white-light endoscopy in the rat colon. (B) After washing twice with PBS, a NIRF signal was confirmed in the excised intestine tissue. (C) The targeting binding of CEA-FSNs to the polyps was cross-validated in tissue sections with immunofluorescence imaging. The NIRF signals from CEA-FSNs (purple) are well overlaid with the signals from a CRC marker (β-catenin, green) and CEA (red) (scale bar = 100 μm).

Article Snippet: The primary antibodies were treated overnight at 4 °C using anti-β-catenin antibody (#8480S, Cell Signaling Technology, Danvers, MA; diluted 1:1000) and anti-CEA antibody (#2383S, Cell Signaling Technology, Danvers, MA; diluted 1:1000).

Techniques: Imaging, Binding Assay, Immunofluorescence, Marker

Journal: Frontiers in chemistry

Article Title: Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode.

doi: 10.3389/fchem.2022.899276

Figure Lengend Snippet: FIGURE 6 | SWV calibration plot of PtμE/Au aptasensors recorded for 0.1 μg ml−1 CEA, 1 μg ml−1 AFP, 1 μg ml−1 BSA, 1 μg ml−1 pancreatin, and 1 μg ml−1 human IgG.

Article Snippet: The CEA protein and alpha-fetoprotein (AFP) protein were purchased from Fitzgerald Inc. Chloroauric acid (HAuCl4) was purchased from Macklin Biotech Co., Ltd. (Shanghai, China).

Techniques:

Journal: medRxiv

Article Title: A Wearable In-pad Diagnostic for the Detection of Disease Biomarkers in Menstruation Blood

doi: 10.1101/2024.03.22.24304704

Figure Lengend Snippet: Design of LFAs suitable for biomarker detection in human serum and whole blood in clinically relevant detection windows. a) Design of CEA LFA assay using human CEA-antibody functionalized 40 nm gold nanoparticles in human serum and b) in fresh, unprocessed human whole blood. c) Design of CA-125 LFA assay using human CA-125-antibody functionalized 40 nm gold nanoparticles in human serum and d) in fresh, unprocessed human whole blood. e) Design of CRP LFA assay using human CRP-antibody functionalized 20 nm gold nanoparticles in human serum and f) in fresh, unprocessed human whole blood. Assays were performed in triplicate using independent blood donors (N=3).

Article Snippet: CRP from human ascites were purchased from Sigma-Aldrich, and CEA proteins were purchased from Fitzgerald industries international.

Techniques: Biomarker Assay