Journal: Lab on a Chip

Article Title: Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays

doi: 10.1039/c8lc00927a

Figure Lengend Snippet: Fig. 1 Microfluidic communicating vessel (μCOVE) chip for rapid magnetic bead-based ELISA. (A) Schematic illustration of the chip design. The three-layer PDMS/glass chip integrates four parallel units of gated communicating vessels, each consisting of seven vessels (∼20 μL each) connected by the flow channels (red) in the bottom PDMS layer. The vessels are gated with an array of normally-closed microvalves actuated by the pneumatic channels (green) in the top layer. The vessel array ends with an enclosed detection microchamber connected to a reservoir for loading RGP. (B) Photograph of an assembled chip filled with red food dye in the communicating vessels and green dye in the pneumatic control channels. (C) Assay workflow. Immunomagnetic microbeads are added into the sample vessel for target capture. After incubation, the first two gates are pneumatically opened one at a time to pull the beads across the next washing buffer vessel with a magnet. The beads are sequentially moved through the vessels containing a biotinylated detection antibody, washing buffer, and streptavidin–β-galactosidase to form immunocomplexes. Finally, the beads are pulled across two washing vessels into the detection microchamber loaded with RGP from the end res- ervoir. The tagged enzyme converts RGP to fluorescent resorufin molecules for quantitative measurement of the bead-captured proteins. (D) Schematic illustration of rapid hydrodynamic washing in a μCOVE chip. The hydrostatic pressure caused by the different liquid levels between two communicating vessels generates a counter flow to wash the beads during traverse. The washed beads are moved across the washing vessels without incubation to expedite the immunoassay. The drawing is not to scale.

Article Snippet: For CEA quantification, 3.2 pg mL−1 to 32 ng mL−1 CEA protein standards (30-1819, Fitzgerald), 43 μg mL−1 biotinylated anti-CEA detection antibody (61-C10GB, Fitzgerald) and 0.2 mM RGP were used in the microchip immunomagnetic assays.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Incubation

Journal: International Journal of Nanomedicine

Article Title: Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

doi: 10.2147/ijn.s30061

Figure Lengend Snippet: Figure 4 SSB examination of a liver tumor. (A) Setup scheme. (B) The scanning curves of all test mice at different times. (C) The variation of magnetism M of all test mice at different times. (D) The analysis comparison of SSB and MRI. Abbreviations: AFP, alphafetaprotein; CEA, carcinoembryonic-antigen; MF, magnetic fluid; SSB, scanning superconducting-quantum-interference-device biosusceptometry; MRI, magnetic resonance imaging.

Article Snippet: Abbreviations: CEA, carcinoembryonic-antigen; MF, magnetic fluid; AFP, alphafetaprotein. submit your manuscript | www.dovepress.com Dovepress Dovepress 2989 Magnetic labeling of magnetic-nanoparticles on tumors In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 76 .1 03 .2 32 .8 7 on 2 5- S ep -2 01 8 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2012:7 using an anti-CEA antibody (10C-CR2014M5, Fitzgerald, MA) instead of an anti-AFP antibody.

Techniques: Comparison, Magnetic Resonance Imaging

Journal: International Journal of Nanomedicine

Article Title: Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

doi: 10.2147/ijn.s30061

Figure Lengend Snippet: Figure 5 HE stain and Prussian blue stain of liver tumor tissue after the injection of MFs (magnification ×400). Abbreviations: HE, hematoxylin and eosin; AFP, alphafetaprotein; MF, magnetic fluid; CEA, carcinoembryonic antigen.

Article Snippet: Abbreviations: CEA, carcinoembryonic-antigen; MF, magnetic fluid; AFP, alphafetaprotein. submit your manuscript | www.dovepress.com Dovepress Dovepress 2989 Magnetic labeling of magnetic-nanoparticles on tumors In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 76 .1 03 .2 32 .8 7 on 2 5- S ep -2 01 8 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2012:7 using an anti-CEA antibody (10C-CR2014M5, Fitzgerald, MA) instead of an anti-AFP antibody.

Techniques: H&E Stain, Staining, Injection

Journal: Cellular and Molecular Life Sciences

Article Title: The BACE1-generated C-terminal fragment of the neural cell adhesion molecule 2 (NCAM2) promotes BACE1 targeting to Rab11-positive endosomes

doi: 10.1007/s00018-022-04575-w

Figure Lengend Snippet: BACE1 is involved in proteolytic processing of NCAM2 in hippocampal neurons. A Schematic diagram showing locations of the domains recognized by antibodies against NCAM2-ED, NCAM2-ID, and HA tag (only in overexpressed NCAM2) used in this study. Orange arrow denotes the BACE1 cleavage site. B Lysates and medium from cultured hippocampal neurons treated with vehicle (0.1% DMSO) or BACE inhibitor analyzed by Western blot with anti-NCAM2-ED antibodies. Levels of NCAM2 are increased in lysates and reduced in the culture medium of neurons treated with the inhibitor. Graph shows NCAM2 levels in lysates ( n = 14) and the ratios of medium/lysate levels ( n = 8) from the inhibitor-treated neurons relative to levels in vehicle-treated neurons set to 100%. Means ± SEM are indicated. * p , one sample t test compared to the vehicle level. C NCAM2-ED and NCAM2-ID labeling in hippocampal neurons co-transfected with GFP and BACE1 siRNA, BACE2 siRNA or control siRNA. Bar = 20 μm. Graphs show mean + SEM NCAM2-ED and NCAM2-ID labeling intensities along dendrites of transfected neurons and their ratios ( n > 46 neurons per group) normalized to the mean of control siRNA-transfected neurons set to 100%. * p , one-way ANOVA with Dunnett’s multiple comparisons test. D NCAM2-ED and NCAM2-ID labeling of CHO cells co-transfected with GFP and NCAM2 and treated with the BACE inhibitor or vehicle (0.1% DMSO). Bar = 20 μm. Graphs show mean + SEM NCAM2-ED and NCAM2-ID labeling intensities and their ratios ( n > 173 cells per group) normalized to the mean of GFP-transfected cells set to 100%. * p , unpaired t -test. E NCAM2-ID and HA tag labeling in CHO cells co-transfected with HA-NCAM2 and GFP or BACE1-FLAG. Note the strongly reduced HA tag labeling in BACE1 co-transfected cells. Bar, 20 μm. Graph shows mean + SEM ratios of the HA tag and NCAM2-ID labeling intensities in CHO cells co-transfected with GFP, BACE1 or BACE2 ( n > 188 cells per group) normalized to the means of GFP-transfected cells set to 100%. * p , one-way ANOVA and Dunnett’s multiple comparisons test

Article Snippet: Bace1 (Myc-Flag-tagged, cat# MR208042) and Bace2 (Myc-Flag-tagged, cat# MR220631) were from OriGene.

Techniques: Cell Culture, Western Blot, Labeling, Transfection, Control