Journal: Frontiers in Microbiology

Article Title: Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (vis+wIRA) and Chlorine e6 (Ce6) Eradicates Planktonic Periodontal Pathogens and Subgingival Biofilms

doi: 10.3389/fmicb.2016.01900

Figure Lengend Snippet: Diagrams of the CFUs depicting the photodynamic efficiency against aerobic and anaerobic oral bacteria within the subgingival biofilm, respectively. Ce6 at a concentration of 100 μg/ml served as the photosensitizer. An untreated negative control and a 0.2% CHX-treated positive control were also tested, along with Ce6-treated biofilms in the absence of vis+wIRA and vis+wIRA-treated biofilms in the absence of Ce6. The CFUs are presented on a Log 10 scale per milliliter (Log 10 /ml). Data shown are means ± SD ( n = 6).

Article Snippet: The photosensitizer used was Ce6 (C 34 H 36 N 4 O 6 , Apocare Pharma GmbH, Bielefeld, Germany).

Techniques: Bacteria, Concentration Assay, Negative Control, Positive Control

Journal: Frontiers in Microbiology

Article Title: Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (vis+wIRA) and Chlorine e6 (Ce6) Eradicates Planktonic Periodontal Pathogens and Subgingival Biofilms

doi: 10.3389/fmicb.2016.01900

Figure Lengend Snippet: Boxplots illustrating percentages of the live bacteria as detected by live/dead staining after the application of photodynamic therapy against the subgingival biofilm. Ce6 at a concentration of 100 μg/ml served as the photosensitizer. An untreated negative control and a 0.2% CHX-treated positive control were also tested, along with Ce6-treated biofilms in the absence of vis+wIRA and vis+wIRA-treated biofilms in the absence of Ce6. The central line represents the median; whiskers indicate minimum and maximum.

Article Snippet: The photosensitizer used was Ce6 (C 34 H 36 N 4 O 6 , Apocare Pharma GmbH, Bielefeld, Germany).

Techniques: Bacteria, Staining, Concentration Assay, Negative Control, Positive Control

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: Schematical showing the preparation of DF with AS1411 G4 motif for Ce6 loading and hemin incorporation for tumor targeting PDT/ferroptosis combinatorial therapy

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Schematic showing the design of template sequence and the preparation of DF. B The TEM micro-images of DF and CH/DF. C The SEM micro-image of CH/DF. D DLS and E surface charge measurements of DF and CH/DF. Inset: the appearance of the nanoparticles after centrifugation. F The fluorescence spectra of Ce6 before and after loading into DF. G The elemental mapping micro-image of CH/DF

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Sequencing, Centrifugation, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Ex vivo imaging the biodistribution of CH/DF and free Ce6 at 12 h post-injection into tumor bearing mice. B The intensity quantification at different organs based on the fluorescence images in A . C Dynamic monitoring the tumor growth after various treatments. D The appearance and E weight of the tumors at day 16 post various treatments. F Tumor H&E staining images, fluorescent staining of TUNEL and caspase-3 of the mice with different treatments

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Ex Vivo, Imaging, Injection, Fluorescence, Staining, TUNEL Assay

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: TGA analyses of the QDs, QD‐ED and QD‐ED‐Ce6

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: Cell viabilities for free Ce6, free QD and QD‐Ce6 complexes (0.5 µmol l−1) before and after X‐ray treatment on SW1353 cells, respectively

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Preparation of LPL and PLCe6. ( a ) Schematic diagram of the structure and chemical synthesis of LPL; ( b ) measurement of the molecular weight of LPL with MALDI-TOF; ( c ) schematic diagram of the structure and chemical synthesis of PLCe6; ( d ) confirmation of the synthesis of LPL1Ce6 and HPL10Ce6 molecules from Ce6 with UV–vis spectroscopy after purification; ( e ) RP-HPLC of Ce6, LPL1Ce6 and HPL10Ce6.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Molecular Weight, UV-Vis Spectroscopy, Purification

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Cellular uptake and cytotoxicity effects of PLCe6 nanoparticles. ( a ) Cellular uptake of PLCe6 nanoparticles in CT26.WT cells treated for 3 h was observed by using fluorescence microscopy (red; Ce6, λEx = 540 nm, λEm = 605 nm, blue; DAPI, λEx = 375 nm, λEm = 460 nm); scale bars = 50 µm for all. ( b ) Cytotoxicity evaluation of Ce6, LPLCe6, and HPLCe6 on CT26.WT cells for 24 h with laser irradiation; ( c ) without laser irradiation ( n = 6), mean ± SD, *** p < 0.001.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Fluorescence, Microscopy, Irradiation