Journal: Scientific Reports

Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

doi: 10.1038/srep20395

Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining

Journal: BMC Genomics

Article Title: Microarray analysis of iron deficiency chlorosis in near-isogenic soybean lines

doi: 10.1186/1471-2164-8-476

Figure Lengend Snippet: Real Time PCR results confirming differential expression identified by microarray analysis

Article Snippet: RNA extracted from root tissue of both Fe efficient and Fe inefficient plants was fluorescently labeled and hybridized to soybean cDNA microarray slides, containing 9,728 cDNAs representing unigene libraries Gm-r1021 and Gm-r1083 [ ], in a balanced dye swap design.

Techniques: Real-time Polymerase Chain Reaction, Quantitative Proteomics, Microarray

Journal: BMC Genomics

Article Title: Microarray analysis of iron deficiency chlorosis in near-isogenic soybean lines

doi: 10.1186/1471-2164-8-476

Figure Lengend Snippet: Whole Root Reductase Assay Results Across Various Iron Concentration Growth Conditions . The iron efficient Clark plant shows a statistically significant increase in reductase activity at 50 uM Fe(NO3)3, iron deficient conditions for the microarray experiment. At the same iron concentration, the iron inefficient IsoClark shows low levels of reductase activity.

Article Snippet: RNA extracted from root tissue of both Fe efficient and Fe inefficient plants was fluorescently labeled and hybridized to soybean cDNA microarray slides, containing 9,728 cDNAs representing unigene libraries Gm-r1021 and Gm-r1083 [ ], in a balanced dye swap design.

Techniques: Reductase Assay, Concentration Assay, Activity Assay, Microarray