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    Illumina Inc cdna libraries
    Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular <t>RNAs.</t> Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in <t>cDNA</t> but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .
    Cdna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 30682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc nextera dna libraries
    Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular <t>RNAs.</t> Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in <t>cDNA</t> but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .
    Nextera Dna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera dna libraries/product/Illumina Inc
    Average 99 stars, based on 124 article reviews
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    99
    Illumina Inc truseq dna library
    Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular <t>RNAs.</t> Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in <t>cDNA</t> but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .
    Truseq Dna Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc complementary dna cdna libraries
    Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular <t>RNAs.</t> Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in <t>cDNA</t> but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .
    Complementary Dna Cdna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 683 article reviews
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    Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular RNAs. Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in cDNA but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .

    Journal: Scientific Reports

    Article Title: Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae

    doi: 10.1038/s41598-018-25242-w

    Figure Lengend Snippet: Sequence features and validation of circRNAs in M. oryzae . ( a ) Venn diagram showing the number of tissue-preferentially expressed circRNAs in mycelium and conidium of M.oryzae . ( b ) Distribution of circRNAs in genome region of M. oryzae . ( c ) Length distribution of circRNAs. ( d ) An example of M. oryzae circRNAs (mor_circ_04492) shows the validation strategy. Divergent and convergent primers were designed to detect circular RNAs. Sanger sequencing was performed to confirm head-to-tail backsplicing. ( e ) Experimental validation of M. oryzae circRNAs. Divergent primers successfully amplified circRNAs in cDNA but failed in genomic DNA. Amplification for sequence of actin gene was used as a control. The gels were cropped from the same gel, and the full-length gel was supported in Fig. S2 .

    Article Snippet: By using an mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the remaining RNAs were used to construct cDNA libraries, which were subsequently used to perform circRNA sequencing by an Illumina Hiseq2500 platform.

    Techniques: Sequencing, Amplification