cdk9-in-7 Search Results


icdk9  (MedChemExpress)
90
MedChemExpress icdk9
Figure 3. THZ1 suppresses the activation of EGFR CAR T-cell–induced enhancer and immunosuppressive genes. A and B, MDA-MB-231 cells incubated with CTL or EGFR CAR T cells were incubated with or without THZ1 (250 nmol/L), JQ1 (100 nmol/L), C646 (20 mmol/L), MG149 (200 mmol/L), KDM5-C70 (5 mmol/L), or <t>iCDK9</t> (5 mmol/L) for 48 (A) or 72 (B) hours. MDA-MB-231 cellswere subjected to qRT-PCR (A) or immunoblotting (B). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. ns, nonsignificant; , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right. C, MDA-MB-231 cells were incubated with or without EGFR CAR T cells in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. D and E, Heat map (D) and box plot (E) representation of the expression (FPKM, log2) for genes that were induced by EGFR CAR T cells, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown atthe top of the box plot; paired Student t test,two-tailed.F, The expression (FPKM, log2)of representativeimmunosuppressive genes from RNA-seq as described in C. G, MDA-MB-231 cells were incubated with or without IFNg (10 ng/mL) in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. H and I, Heat map (H) and box plot (I) representation of the expression (FPKM, log2) for genes that were induced by IFNg, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown at the top of the box plot; paired Student t test, two-tailed. J, The expression (FPKM, log2) of representative immunosuppressive genes from RNA-seq as described in G. K–N, MDA-MB-231 cells as described in C or G were subjected to qRT-PCR (K and L) or immunoblotting (M and N). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right.
Icdk9, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk9-in-7  (MuseChem Chemicals)
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CDK9-IN-7（Cat No.:I018694）is a selective inhibitor of cyclin-dependent kinase 9 (CDK9), an enzyme crucial for regulating transcription and cell cycle progression. By inhibiting CDK9, this compound disrupts the transcriptional machinery that cancer cells rely on for
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cdk9-in-7  (TargetMol)
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cdk9-in-7  (Biosynth Carbosynth)
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cdk9-in-7  (Aladdin Scientific Corporation)
N/A
CDK9-IN-7 (compound 21e) is a selective, highly potent, and orally active CDK9/cyclin T inhibitor ( IC 50 =11 nM), which exhibits more potent over other CDKs (CDK4/cyclinD=148 nM; CDK6/cyclinD=145 nM). CDK9-IN-7 shows antitumor activity without
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Figure 3. THZ1 suppresses the activation of EGFR CAR T-cell–induced enhancer and immunosuppressive genes. A and B, MDA-MB-231 cells incubated with CTL or EGFR CAR T cells were incubated with or without THZ1 (250 nmol/L), JQ1 (100 nmol/L), C646 (20 mmol/L), MG149 (200 mmol/L), KDM5-C70 (5 mmol/L), or iCDK9 (5 mmol/L) for 48 (A) or 72 (B) hours. MDA-MB-231 cellswere subjected to qRT-PCR (A) or immunoblotting (B). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. ns, nonsignificant; , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right. C, MDA-MB-231 cells were incubated with or without EGFR CAR T cells in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. D and E, Heat map (D) and box plot (E) representation of the expression (FPKM, log2) for genes that were induced by EGFR CAR T cells, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown atthe top of the box plot; paired Student t test,two-tailed.F, The expression (FPKM, log2)of representativeimmunosuppressive genes from RNA-seq as described in C. G, MDA-MB-231 cells were incubated with or without IFNg (10 ng/mL) in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. H and I, Heat map (H) and box plot (I) representation of the expression (FPKM, log2) for genes that were induced by IFNg, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown at the top of the box plot; paired Student t test, two-tailed. J, The expression (FPKM, log2) of representative immunosuppressive genes from RNA-seq as described in G. K–N, MDA-MB-231 cells as described in C or G were subjected to qRT-PCR (K and L) or immunoblotting (M and N). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right.

Journal: Cancer Immunology Research

Article Title: Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition

doi: 10.1158/2326-6066.cir-20-0405

Figure Lengend Snippet: Figure 3. THZ1 suppresses the activation of EGFR CAR T-cell–induced enhancer and immunosuppressive genes. A and B, MDA-MB-231 cells incubated with CTL or EGFR CAR T cells were incubated with or without THZ1 (250 nmol/L), JQ1 (100 nmol/L), C646 (20 mmol/L), MG149 (200 mmol/L), KDM5-C70 (5 mmol/L), or iCDK9 (5 mmol/L) for 48 (A) or 72 (B) hours. MDA-MB-231 cellswere subjected to qRT-PCR (A) or immunoblotting (B). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. ns, nonsignificant; , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right. C, MDA-MB-231 cells were incubated with or without EGFR CAR T cells in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. D and E, Heat map (D) and box plot (E) representation of the expression (FPKM, log2) for genes that were induced by EGFR CAR T cells, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown atthe top of the box plot; paired Student t test,two-tailed.F, The expression (FPKM, log2)of representativeimmunosuppressive genes from RNA-seq as described in C. G, MDA-MB-231 cells were incubated with or without IFNg (10 ng/mL) in the presence or absence of THZ1 (250 nmol/L) for 48 hours, followed by RNA-seq analysis. H and I, Heat map (H) and box plot (I) representation of the expression (FPKM, log2) for genes that were induced by IFNg, but suppressed by THZ1. Box plot shows median (line), top and bottom quartiles (boxes), and lines extending to highest and lowest observations (whiskers). P values are shown at the top of the box plot; paired Student t test, two-tailed. J, The expression (FPKM, log2) of representative immunosuppressive genes from RNA-seq as described in G. K–N, MDA-MB-231 cells as described in C or G were subjected to qRT-PCR (K and L) or immunoblotting (M and N). Experiments were repeated three times, and representative data are shown. Statistical significance is shown in Supplementary Table S2. , P < 0.05; , P < 0.01; , P < 0.001; unpaired Student t test, two-tailed. Molecular weight is indicated on the right.

Article Snippet: Specifically, cells listed below were subjected to immunoblotting: MDAMB-231 cells incubated with CTL T or EGFR CAR T cells at a ratio of Cancer Immunol Res; 9(6) June 2021 CANCER IMMUNOLOGY RESEARCH710 D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/9/6/707/3099359/707.pdf by guest on 25 April 2024 2:1 were incubated with or without THZ1 (250 nmol/L, HY-80013, MedChemExpress), JQ1 (100 nmol/L, HY-13030,MedChemExpress), C646 (20 mmol/L, HY-13823, MedChemExpress), MG149 (200 mmol/ L, HY-15887, MedChemExpress), KDM5-C70 (5 mmol/L, T15648, TOPSCIENCE), or iCDK9 (5 mmol/L, HY-126251, MedChemExpress) for 72 hours; MDA-MB-231 or MDA-MB-468 cells were incubated with CTL T or EGFR CAR T cells at a ratio of 2:1 and then incubated with or without THZ1 (250 nmol/L) for 72 hours; MDA-MB-231 or MDA-MB-468 cells were incubated with IFNg (10 ng/mL) in the presence or absence of THZ1 (250 nmol/L) for 72 hours; MDA-MB-231 or MDA-MB-468 cells transfected with siCTL or siCDK7 were incubated with EGFR CAR T cells at a ratio of 2:1 or IFNg (10 ng/mL) for 72 hours; MDA-MB-231 cells were transduced with lentivirus-containing control vector (PCDH-CTL 231), human PD-L1 (PCDH-PDL1 231), IDO1 (PCDH-IDO1 231), or HVEM (PCDH-HVEM 231) for 48 hours; MDA-MB-231 cells were transduced with lentivirus-containing sgCTL, sgSTAT1, or sgCDK7 for 48 hours.

Techniques: Activation Assay, Incubation, Quantitative RT-PCR, Western Blot, Two Tailed Test, Molecular Weight, RNA Sequencing, Expressing