Journal: Scientific reports

Article Title: PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer.

doi: 10.1038/s41598-024-59427-3

Figure Lengend Snippet: Figure 6. PAFAH1B3 affects the expression of EMT-related proteins in pancreatic cancer cells (A) SW1990 and MIA Paca-2 cells were transduced with lentivirus containing PAFAH1B3-Flag or NC, and the protein expression levels of PAFAH1B3, E-cadherin, N-cadherin, Vimentin, Snail1 and MMP2 were measured via Western blotting. (B) SW1990 and MIA Paca-2 cells were transduced with lentivirus containing sh-PAFAH1B3 or sh-NC, and the protein expression levels of PAFAH1B3, E-cadherin, N-cadherin, Vimentin, Snail1 and MMP2 were measured via Western blotting. β-Actin was used as an internal control. The data represent the average of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The following antibodies were used: rabbit anti-PAFAH1B3 polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), mouse anti-β-actin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-PCNA polyclonal antibody (1:1000; Abcam, Cambridge, MA, USA), rabbit anti-E-cadherin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-Ncadherin polyclonal antibody (1:1000; Boster Biological Technology, Ltd), rabbit anti-Snail1 polyclonal antibody 3 Vol.

Techniques: Expressing, Transduction, Western Blot, Control

Journal: Cell reports

Article Title: BCL2 drives castration resistance in castration-sensitive prostate cancer by orchestrating reciprocal crosstalk between oncogenic pathways

doi: 10.1016/j.celrep.2025.115779

Figure Lengend Snippet: (A) Schema of the experimental design for the cell-growth assay using isogenic pairs of LNCaP, control (empty vector overexpressed), and BCL2-overexpressed (BCL2-OE) LNCaP cells. (B) The bar graph represents cell growth under indicated conditions in control and BCL2-OE LNCaP cells. Error bar, SD; unpaired t test. Data represent eight biological replicates. (C) Representative phase contrast (scale bar, 500 μm) and immunofluorescence staining of E-cadherin, β-catenin, and vimentin (scale bar, 90 μm) of isogenic pairs of LNCaP cells under indicated conditions. Nuclei were stained with DAPI (blue). n = 3 biological replicates. (D) qPCR of the indicated transcriptome in the isogenic pairs of LNCaP cells either cultured in complete medium or in ADT (CSS) for a prolonged time (28 days). n = 3 biological replicates. Error bar, SD. (E) Left: the Venn diagram represents differentially expressed upregulated hallmark-signaling pathways (based on the RNA-seq) in the isogenic pairs of LNCaP cells when cultured under prolonged ADT. Right: enrichment plot showing upregulation of hallmark Hedgehog signaling pathway in BCL2-OE LNCaP cells when exposed under prolonged ADT. (F) Isogenic pairs of LNCaP cells (control and BCL2-OE) either cultured in complete medium (FBS) or in ADT (CSS) for 4 days and then treated with vismodegib (Hedgehog inhibitor) for another 7 days with indicated concentrations. The bar graph represents the percentage of cells growth inhibition in response to vismodegib treatment. Error bar, SD; unpaired t test. Data represent eight biological replicates. (G) Schema representing ADT-induced cellular plasticity through ADT-induced feedforward loop between BCL2 and Hedgehog and followed by activation of Hedgehog signaling pathway.

Article Snippet: E cadherin; Human , Thermofisher Scientific , Cat#Hs01023895_m1.

Techniques: Growth Assay, Control, Plasmid Preparation, Immunofluorescence, Staining, Cell Culture, Protein-Protein interactions, RNA Sequencing, Inhibition, Activation Assay