Journal: Journal of nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration.

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Fig. 1 Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 107 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on rat SVF cells. a Cell size (μm) of the SVF cells, demonstrating no difference between the three groups. b Percentage of colony-forming cells, showing significantly fewer colonies in the 1D group (6.1 ± 1.6 %) compared to the Control (11.4 ± 1.8 %) and 7D groups (11.0 ± 0.9 %). c Number of cells, obtained 4 days after seeding of the SVF fraction, demonstrating significantly fewer cells in the 1D group (1.3 ± 0.1 million cells) compared to the Control (2.0 ± 0.1 million cells) and 7D groups (1.8 ± 0.1 million cells). d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271 showing significantly fewer CD90- and CD105-positive cells in the 1D group. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5, * p < 0.05, ** p < 0.01)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Control

Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on cultured rat ASC. a Representative light microscopy image demonstrating the morphology of cultured rat ASC. b Cell size (μm) of ASC, demonstrating no significant differences between the three groups. c Population doubling time (in days) depicted for passages 1–3, showing no significant differences between the three groups. d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271, showing no significant differences in numbers of positive cells between the groups. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Cell Culture, Light Microscopy, Control