cd90 2 Search Results


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Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
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Elabscience Biotechnology cd90 2 pe
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
Cd90 2 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech his tag mouse monoclonal antibody
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
His Tag Mouse Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Proteintech rat anti rfp
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
Rat Anti Rfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProSci Incorporated csptpqrrpvssihppgrppa
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
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93
fluidigm anti cd90 2 156gd
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
Anti Cd90 2 156gd, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech mouse anti cd90
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
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Proteintech anti cd90 mouse monoclonal antibody
Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells <t>(CD90.2</t> + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).
Anti Cd90 Mouse Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells (CD90.2 + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).

Journal: Scientific Reports

Article Title: The proton-sensing G protein-coupled receptor T-cell death-associated gene 8 (TDAG8) shows cardioprotective effects against myocardial infarction

doi: 10.1038/s41598-017-07573-2

Figure Lengend Snippet: Comparison of TDAG8 + infiltrating cells in infarcted hearts of WT mice and TDAG8 KO mice. ( a ) Tdag8 mRNA levels in T cells (T cell), neutrophils (Neu), macrophages (Mac), fibroblasts (Fib) and other cell types isolated from hearts of WT mice on post-MI day 3. Three infarcted WT mice hearts were stained with each antibody. After eliminating dead cells and gating singlet live cells, T cells (CD45.2 + Ly6G − CD11b − CD3 + ), neutrophils (CD45.2 + Ly6G + CD11b + CD3 − ), macrophages (CD45.2 + Ly6G − CD11b + CD3 − ), fibroblasts (CD45.2 − PDGFRα + ) and other cells (CD45.2 − PDGFRα − ) were sorted. The total mRNA collected from each cell population was used for real-time RT-PCR analysis. ( b ) Comparison of infiltrating T cells (CD90.2 + ), macrophages (Ly6G − CD11b + ) and neutrophils (Ly6G + CD11b + ) on post-MI day 3 between WT mice (n = 4–7) and TDAG8 KO mice (n = 4) Representative FACS profiles are shown. Comparisons were assessed using unpaired Student’s t -tests. Error bars represent the mean ± SEM (N.S., not significant).

Article Snippet: After isolated cardiac cells were treated with mouse Fc Block TM (BD), samples were stained with anti-CD45.2-Alexa Fluor 488 (BioLegend), anti-CD45.2-APC (BioLegend), anti-Ly6G-PE-Cy7 (BioLegend), anti-CD11b-PerCP-Cy5.5 (BioLegend), anti-CD11b-PE (BD), anti-PDGFRα-APC (BioLegend), anti-CD3ε-PE (BD), anti-CD3ε-PE-Cy5 (TONBO Biosciences), anti-CD4-APC-Cy7 (TONBO Biosciences), anti-CD90.2-APC (eBiosceince), anti-γδTCR-PE (BD), anti-CCR6-APC (RD Systems), anti-B220-APC (BioLegend).

Techniques: Isolation, Staining, Quantitative RT-PCR