Journal: Frontiers in Genetics

Article Title: Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy

doi: 10.3389/fgene.2018.00072

Figure Lengend Snippet: Coexpression of CD90 and CD117. Two serial sections of the same patient (Patient 4) are stained with different antibodies ( A , CD90; B , CD117). The circle shows cells with co-expression of both stem cell markers (CD90 + on 1st slide, CD117 + on 2nd slide).

Article Snippet: Primary antibodies, in specific, CD90 [anti-CD90/Thy-1 antibody, rabbit monoclonal IgG, clone EPR3133, ab 133350 (dilution 1:100) Abcam Cambridge, UK] and CD117 [anti-c-kit/CD117 antibody, rabbit monoclonal IgG, clone YR145, ab 32363 (dilution 1:50) Abcam Cambridge, UK] were added and incubated overnight at 4°C.

Techniques: Staining, Expressing

Journal: Frontiers in Genetics

Article Title: Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy

doi: 10.3389/fgene.2018.00072

Figure Lengend Snippet: Co-expression of CD90, CD117, and CD105. Three serial sections of the same patient (Patient 4) are stained with different antibodies: CD90, CD117, and CD105. The circles mark cells, which are positive for all three antibodies.

Article Snippet: Primary antibodies, in specific, CD90 [anti-CD90/Thy-1 antibody, rabbit monoclonal IgG, clone EPR3133, ab 133350 (dilution 1:100) Abcam Cambridge, UK] and CD117 [anti-c-kit/CD117 antibody, rabbit monoclonal IgG, clone YR145, ab 32363 (dilution 1:50) Abcam Cambridge, UK] were added and incubated overnight at 4°C.

Techniques: Expressing, Staining

Journal: Frontiers in Genetics

Article Title: Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy

doi: 10.3389/fgene.2018.00072

Figure Lengend Snippet: A number of cells with co-expression of CD90 and CD117 per square millimeter ( n = 12). The data are expressed as mean ± standard deviation. The Myocarditis patient group displayed the most cells with co-expression of both stem cell markers.

Article Snippet: Primary antibodies, in specific, CD90 [anti-CD90/Thy-1 antibody, rabbit monoclonal IgG, clone EPR3133, ab 133350 (dilution 1:100) Abcam Cambridge, UK] and CD117 [anti-c-kit/CD117 antibody, rabbit monoclonal IgG, clone YR145, ab 32363 (dilution 1:50) Abcam Cambridge, UK] were added and incubated overnight at 4°C.

Techniques: Expressing, Standard Deviation

Journal: Fibrogenesis & Tissue Repair

Article Title: HDAC class I inhibitor, Mocetinostat, reverses cardiac fibrosis in heart failure and diminishes CD90+ cardiac myofibroblast activation

doi: 10.1186/1755-1536-7-10

Figure Lengend Snippet: HDAC2 is co-localized with cardiac fibroblast in the infarcted and non-infarcted myocardium in CHF. Coronal (LA) and axial (LV, RV) sections of sham and 6w CHF hearts were stained for HDAC2 (A-G) and fibroblast marker CD90. DAPI (Blue) is used to stain nuclei. CD90 and HDAC2 staining were co-localized in remote LV, RV, and LA and infarcted myocardium. White arrows indicate co-localization of CD90+ cells with HDAC2. Scale bars: 100 μm. CHF, congestive heart failure; HDAC, Histone Deacetylase; LA, left atrium; LV, left ventricle; RV, right ventricle.

Article Snippet: Click here for file Additional file 2 CD90+ cells express myofibroblast markers. (A) Flow cytometry analysis of CD90+ cells.

Techniques: Staining, Marker, Histone Deacetylase Assay

Journal: Fibrogenesis & Tissue Repair

Article Title: HDAC class I inhibitor, Mocetinostat, reverses cardiac fibrosis in heart failure and diminishes CD90+ cardiac myofibroblast activation

doi: 10.1186/1755-1536-7-10

Figure Lengend Snippet: HDAC inhibition induced MET-like process in cardiac fibroblasts. Atrial CD90+ cells were treated with Mocetinostat for 7 days. (A) Transmitted light images demonstrate changes in cell morphology upon treatment with Mocetinostat. Scale bar: 100 μm. (B) Cells were labeled with antibodies to α-SMA (red) and β-catenin (green). DAPI (Blue) is used to stain nuclei. Scale bar: 50 μM. (C) CD90+ cells treated with Mocetinostat were lysed and western blot analysis was performed against E-cadherin and β-catenin. Graphs show the density analysis to corresponding to specific bands normalized to β-actin. (D) Western blot analysis for Akt and GSK3β signaling. Error bars indicate S.E. (n = 4) P

Article Snippet: Click here for file Additional file 2 CD90+ cells express myofibroblast markers. (A) Flow cytometry analysis of CD90+ cells.

Techniques: Inhibition, Labeling, Staining, Western Blot

Journal: Fibrogenesis & Tissue Repair

Article Title: HDAC class I inhibitor, Mocetinostat, reverses cardiac fibrosis in heart failure and diminishes CD90+ cardiac myofibroblast activation

doi: 10.1186/1755-1536-7-10

Figure Lengend Snippet: HDAC inhibition diminished α-SMA activation in cardiac fibroblast. CD90+ cells were isolated from atrial explants after 21 days in culture. (A) Cells were treated with Mocetinostat for 7 days. Relative gene expressions were measured with real-time PCR. Mocetinostat treatment elevated E-cadherin levels, while reducing α-SMA, MMP2, and Collagen-III. (B) CD90 cells isolated from ventricles were treated with Mocetinostat for 7 days. Levels of α-SMA, MMP2, and Collagen-III were reduced with Mocetinostat treatment, while E-cadherin expression was unaltered. (C) Western blot analysis of α-SMA in Mocetinostat treated atrial and ventricular CD90+ cells. Error bars indicate S.E. (n = 4). P

Article Snippet: Click here for file Additional file 2 CD90+ cells express myofibroblast markers. (A) Flow cytometry analysis of CD90+ cells.

Techniques: Inhibition, Activation Assay, Isolation, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: Fibrogenesis & Tissue Repair

Article Title: HDAC class I inhibitor, Mocetinostat, reverses cardiac fibrosis in heart failure and diminishes CD90+ cardiac myofibroblast activation

doi: 10.1186/1755-1536-7-10

Figure Lengend Snippet: Mocetinostat treatment elevated levels of p21/p53 and Caspase-3 in cardiac fibroblasts. CD90+ cells were treated with Mocetinostat for 7 days. (A) Gene expression of p21, p53, and p16 was analyzed with real-time PCR. Mocetinostat induced expression of p21 and p53 in CD90+ cells. (B) Cleaved Caspase-3 levels were measured with western blot analysis. Mocetinostat induced upregulation of Caspase-3 levels in CD90+ cells. (C) Reduction in cell numbers upon Mocetinostat treatment was quantified by cell counting. Error bars indicate S.E. (n = 4) P

Article Snippet: Click here for file Additional file 2 CD90+ cells express myofibroblast markers. (A) Flow cytometry analysis of CD90+ cells.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Cell Counting

Journal: Fibrogenesis & Tissue Repair

Article Title: HDAC class I inhibitor, Mocetinostat, reverses cardiac fibrosis in heart failure and diminishes CD90+ cardiac myofibroblast activation

doi: 10.1186/1755-1536-7-10

Figure Lengend Snippet: HDAC1 is co-localized with cardiac fibroblast in the infarcted and non-infarcted myocardium in CHF. Coronal (LA) and axial (LV, RV) sections of sham and 6w CHF hearts were stained for HDAC1 (A-G) and fibroblast marker CD90. DAPI (Blue) is used to stain nuclei. CD90 and HDAC1 staining were co-localized in remote LV, RV, and LA and infarcted myocardium. White arrows indicate co-localization of CD90+ cells with HDAC1. Scale bars: 100 μm. CHF, congestive heart failure; LA, left atrium; HDAC, Histone Deacetylase; LV, left ventricle; RV, right ventricle.

Article Snippet: Click here for file Additional file 2 CD90+ cells express myofibroblast markers. (A) Flow cytometry analysis of CD90+ cells.

Techniques: Staining, Marker, Histone Deacetylase Assay