Journal: iScience

Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

doi: 10.1016/j.isci.2025.112458

Figure Lengend Snippet: Akk improves gut immune responses in obese mice (A) PHATE of T cells clustering in mice intestinal lymph nodes. (B–E) Percentages of CD3 + CD4 + Tregs, CD3 + CD8 + Tregs, CD4 + CD25 + Tregs, and CD4 + CD25 + Foxp3 + Tregs. Data are expressed as mean ± SD ( n = 6), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.

Article Snippet: Anti-Mouse CD8 + FITC , BioGems , Cat# 10112-50.

Techniques:

Journal: iScience

Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

doi: 10.1016/j.isci.2025.112458

Figure Lengend Snippet: Gut fungi is the key in Akk improving gut immune responses in organoids (A) Co-culture of colonic organoids, fecal microbiota and Akk. (B) Fluorescent images of colonic organoids with DAPI-labeled cells, and number of organoid crypts. Scale bars: 100 μm. (C) Relative abundance of fungal in co-culture system. (D) Percentages of CD3 + CD4 + , CD3 + CD8 + , CD4 + CD25 + , and CD4 + CD25 + Foxp3 + Tregs in organoids. (E) Relative content of metabolites in co-culture system. (F) Co-culture of colonic organoids, fluconazole, fecal microbiota and Akk. (G) Fluorescent images of colonic organoids with DAPI-labeled cells, and number of organoid crypts. Scale bars: 50 μm. (H) Percentages of CD3 + CD4 + , CD3 + CD8 + , CD4 + CD25 + , and CD4 + CD25 + Foxp3 + Tregs in organoids. (I and J) Relative content of metabolites (α-ketoisovaleric acid and glycylleucine) in co-culture system. Figures 7 A–7E and Figures 7 F–7J represent experiments from different batches, respectively. Data are expressed as mean ± SD (n = 6–8), ns p >0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.

Article Snippet: Anti-Mouse CD8 + FITC , BioGems , Cat# 10112-50.

Techniques: Co-Culture Assay, Labeling