Journal: ACS sensors

Article Title: Development of a Microfluidic Flow Cytometer with a Uniform Optical Field (Uni-μFCM) Enabling Quantitative Analysis of Single-Cell Proteins and Its Applications in Leukemia Gating, Tumor Classification, and Hierarchy of Cancer Stem Cells.

doi: 10.1021/acssensors.3c01060

Figure Lengend Snippet: Figure 4. Uni-μFCM quantified single-cell membrane and cytoplasmic markers of myeloid and lymphocytic leukocytes with correspondingly quantitative identification systems established. (A) Scatter plots of single-cell expressions of CD3, CD79a, and LAMP1 from a T-lymphocytic cell line Jurkat, a B-lymphocytic cell line BALL-1, and a myeloid cell line HL-60. (B) Quantitative identifications of Jurkat, BALL-1, and HL-60 mainly based on single-cell expressions of CD3 and CD79a. (C) Scatter plots of single-cell expressions of CD3, CD79a, and LAMP1 from a healthy donor and a patient blood sample. (D) Quantitative identifications of T-lymphocytes, B-lymphocytes, and leukocytes with the myeloid source based on single-cell expressions of CD3 and CD79a as well as cell diameters.

Article Snippet: Fluorescence-labeled antibodies used for cell staining included antibodies of OCT4-Alexa 488 purchased from Thermo Fisher, USA, CD3-Alexa 488, XRCC3-Alexa 488, c-Myc-PE, CD79a-PE, Cdc20-PE, KLF4-PerCP, LAMP1-PerCP, and BubR1-PerCP purchased from NOVUS, USA.

Techniques: Membrane

Journal: Animals : an Open Access Journal from MDPI

Article Title: Postpartum Uterine Involution in Cows: Quantitative Assessment of Structural Remodeling and Immune Cell Infiltration

doi: 10.3390/ani15172520

Figure Lengend Snippet: Bland–Altman plot showing the differences between manual and automated quantification of positively marked cells in the bovine endometrium for CD3 ( A ), CD79 ( B ), CD204 ( C ) and CD335 ( D ) during the postpartum period, plotted against the average of the two methods (pink dots). The bias is indicated by the distance between the zero-difference line ( X -axis) and the solid green line. The limits of agreement are represented by the dashed lines parallel to the X -axis. Numerical values for the bias and limits of agreement are displayed adjacent to their respective lines.

Article Snippet: CD79 , Novus Biologicals; Centennial, CO, USA (NB-1006434 7) , MoM , 1:100.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Postpartum Uterine Involution in Cows: Quantitative Assessment of Structural Remodeling and Immune Cell Infiltration

doi: 10.3390/ani15172520

Figure Lengend Snippet: Endometrial biopsies of bovine uterus. Immunostaining and quantification of T cells using CD3 (1:100) and B-cells using CD79 (1:100) and counterstained with Hematoxylin. ( A – D ) Negative controls, stained with Hematoxylin only, are the same for all antibodies and were used to confirm tissue morphology preservation. ( E – H ) Endometrial biopsies of bovine uterus. Immunostaining for CD3 on Days 2, 7, 21, and 35 postpartum, respectively. ( I – L ) Endometrial biopsies of bovine uterus. Immunostaining for CD79 on Days 2, 7, 21, and 35 postpartum, respectively. The images were obtained using Aperio ImageScope x64 12.4.6 software (Leica Biosystems), magnification 400x, scale 25 µm. ( M , N ) Manual ( M ) and automatic ( N ) counting of CD3-positive cells. ( O , P ) Manual ( O ) and automatic ( P ) counting of CD79-positive cells. Different letters are statistically significant ( p < 0.05). Results are presented as mean ± standard error of the mean (S.E.M.).

Article Snippet: CD79 , Novus Biologicals; Centennial, CO, USA (NB-1006434 7) , MoM , 1:100.

Techniques: Immunostaining, Staining, Preserving, Software