Journal: PLoS ONE
Article Title: Ensemble of Gene Signatures Identifies Novel Biomarkers in Colorectal Cancer Activated through PPAR? and TNF? Signaling
Figure Lengend Snippet: TMAs and western blot validation analysis of AKAP12, DCBLD2, NT5E and SPON1. a . “Columns from left to right” indicate immunostaining pattern in normal colonic samples and CRC cores negative and positive for each marker AKAP12, DCBLD2, NT5E, SPON1. Black arrows indicate immunohistochemical staining pattern in normal or malignant colonic cells. White arrows indicate the immunostaining distribution in the stromal compartment (endothelial, regulatory T-cells and macrophages) characteristic of NT5E expression pattern. Magnification 10X; b . Number of positive cases detected on TMA validation series comprising tumor specimens (TUM) and a subgroup of matched normal colonic mucosa (NM). Error bars indicate the standard deviation from the mean ( p
Article Snippet: The sections were incubated with 3% hydrogen peroxide for 20 minutes at room temperature and subsequently with the specific antibodies: AKAP12 (dilution 1:500; WH0009590M1, mouse monoclonal, Sigma-Aldrich, St. Louis, MO), Spondin1 (dilution 1:250; AB40797; rabbit polyclonal, Abcam, Cambridge, UK) ); NT5E (dilution 1:50; AB115289; rabbit polyclonal, Abcam, Cambridge, UK); and DCBLD2 (dilution 1:100; AB115451; rabbit polyclonal, Abcam, Cambridge, UK) all antibodies were incubated overnight at 4°C.
Techniques: Western Blot, Immunostaining, Marker, Immunohistochemistry, Staining, Expressing, Standard Deviation