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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: β2-and β3-Adrenergic Receptors Contribute to Cancer-Evoked Pain in a Mouse Model of Osteosarcoma via Modulation of Neural Macrophages
doi: 10.3389/fphar.2021.697912
Figure Lengend Snippet: Tumor growth and mechanical allodynia induced by K7M2 osteosarcoma cells in BALB/c mice (A and B) Time dependent increase in tumor size and mechanical allodynia after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice (C) Representative gating strategy of flow cytometry analysis and relative quantification of F4/80 + /CD64 + monocytes/macrophages in tibial nerve, and (D) typical images and pooled data of F4/80 + cells in the ipsilateral tibial nerve (stained with the neural marker PGP9.5) after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice. BL, baseline. PWT, paw withdrawal threshold. N = 6 mice. * p < 0.05, *** p < 0.001 K7M2 vs Sham. Data are presented as mean ± SEM, data points overlaid (C, D). Two-way ANOVA and Bonferroni post hoc test (A, B), unpaired student t-test (C, D).
Article Snippet: Cells were then stained with anti-CD45-VioBlue conjugated antibody (130-110-664, Miltenyi Biotec), anti-F4/80-PE conjugated antibody (130-116-449, Miltenyi Biotec), and
Techniques: Flow Cytometry, Quantitative Proteomics, Staining, Marker
Journal: Frontiers in Pharmacology
Article Title: β2-and β3-Adrenergic Receptors Contribute to Cancer-Evoked Pain in a Mouse Model of Osteosarcoma via Modulation of Neural Macrophages
doi: 10.3389/fphar.2021.697912
Figure Lengend Snippet: β2-and β3-AR antagonists reduce neuroinflammation in tibial nerve of K7M2 osteosarcoma-bearing mice (A) Representative flow cytometry analysis and relative quantification of F4/80 + /CD64 + macrophages (B) typical immunofluorescence images and pooled data of F4/80 + cells and (C) H 2 O 2 content in the tibial nerve after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice treated (daily, starting from day 10) with atenolol (10 mg/kg, i.p.) propranolol (10 mg/kg, i.p.), SR59230A (10 mg/kg, i.p.) or vehicle (Veh) (D) H 2 O 2 level in RAW 264.7 stimulated with PMA (16 μM) in presence of SR59230A, propranolol, atenolol (all, 100 nM) or Veh (n = 3 independent experiments) (E) Mechanical allodynia at day 14 after para-tibial K7M2 osteosarcoma cell inoculation or sham in BALB/c mice treated with PBN (100 mg/kg, i.p.) or Veh. BL, baseline. PWT, paw withdrawal threshold. N = 6 mice. * p < 0.05, *** p < 0.001 vs Sham, Veh-Veh; § p < 0.05, §§§ p < 0.001 vs K7M2 + Veh, Veh-PMA, K7M2 + Veh PBN. Data are presented as mean ± SEM. One-way (A–C) or two-way (D–E) ANOVA and Bonferroni post hoc test.
Article Snippet: Cells were then stained with anti-CD45-VioBlue conjugated antibody (130-110-664, Miltenyi Biotec), anti-F4/80-PE conjugated antibody (130-116-449, Miltenyi Biotec), and
Techniques: Flow Cytometry, Quantitative Proteomics, Immunofluorescence