Journal: Nature Neuroscience

Article Title: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models

doi: 10.1038/s41593-025-02062-6

Figure Lengend Snippet: Muscle-EV release enriched in postsynaptic compartments . Representative live imaging video of a postsynaptic compartment of a primary skeletal muscle expressing CD63-pHluorin in coculture with primary motor neurons. Video depicts a skeletal muscle-expressed CD63-pHluorin signal is enriched in postsynaptic BTX-positive regions. Green indicates CD63-GFP, magenta indicates BTX. Insets focus on a BTX positive patch in the muscle. Scale bar: 10 µm.

Article Snippet: CD63-peGFP C2 (plasmid no. 62964), pCMV-lyso-pHluorin (CD63-pHluorin; plasmid no. 70113) and pLKO-Tet-On-shRab27a (plasmid no. 120930) were acquired from Addgene repositories. pLL3.7-Ago2-eGFP was constructed in the Tel Aviv University lentiviral facility. pLL3.7-TDP-43-eGFP was constructed by cloning TDP-43-eGFP into PLL3.7 backbone (acquired from Addgene).

Techniques:

Journal: Nature Neuroscience

Article Title: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models

doi: 10.1038/s41593-025-02062-6

Figure Lengend Snippet: Muscle derived CD-63 colocalizes and co-transports with axonal lysosomes . Representative live imaging video of muscle-derived CD63-GFP and lysotracker co-transport in axons within a co-culture with CD63-GFP expressing primary skeletal muscles. Magenta indicates lysotracker, green indicates CD63-GFP. Yellow arrowheads indicate co-transported CD-63 and lysotracker. Scale bar: 5 µm.

Article Snippet: CD63-peGFP C2 (plasmid no. 62964), pCMV-lyso-pHluorin (CD63-pHluorin; plasmid no. 70113) and pLKO-Tet-On-shRab27a (plasmid no. 120930) were acquired from Addgene repositories. pLL3.7-Ago2-eGFP was constructed in the Tel Aviv University lentiviral facility. pLL3.7-TDP-43-eGFP was constructed by cloning TDP-43-eGFP into PLL3.7 backbone (acquired from Addgene).

Techniques:

Journal: Nature Neuroscience

Article Title: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models

doi: 10.1038/s41593-025-02062-6

Figure Lengend Snippet: Muscle-EV release enriched in postsynaptic compartments . Representative live imaging video of a postsynaptic compartment of a primary skeletal muscle expressing CD63-pHluorin in coculture with primary motor neurons. Video depicts a skeletal muscle-expressed CD63-pHluorin signal is enriched in postsynaptic BTX-positive regions. Green indicates CD63-GFP, magenta indicates BTX. Insets focus on a BTX positive patch in the muscle. Scale bar: 10 µm.

Article Snippet: CD63-peGFP C2 (plasmid no. 62964), pCMV-lyso-pHluorin (CD63-pHluorin; plasmid no. 70113) and pLKO-Tet-On-shRab27a (plasmid no. 120930) were acquired from Addgene repositories. pLL3.7-Ago2-eGFP was constructed in the Tel Aviv University lentiviral facility. pLL3.7-TDP-43-eGFP was constructed by cloning TDP-43-eGFP into PLL3.7 backbone (acquired from Addgene).

Techniques:

Journal: Nature Neuroscience

Article Title: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models

doi: 10.1038/s41593-025-02062-6

Figure Lengend Snippet: Muscle-EV uptake by a presynaptic motor axon . Representative live imaging video of a primary skeletal muscle expressing CD63-GFP in contact with a primary motor neuron axon. Video depicts a skeletal muscle-derived CD63-GFP tethered and taken by an axonal growth-cone. Magenta indicates CD63-GFP, green indicates cholera toxin subunit B-conjugated Alexa-647 in motor axons. Arrowhead indicates muscle-derived EVs. Scale bar: 10 µm.

Article Snippet: CD63-peGFP C2 (plasmid no. 62964), pCMV-lyso-pHluorin (CD63-pHluorin; plasmid no. 70113) and pLKO-Tet-On-shRab27a (plasmid no. 120930) were acquired from Addgene repositories. pLL3.7-Ago2-eGFP was constructed in the Tel Aviv University lentiviral facility. pLL3.7-TDP-43-eGFP was constructed by cloning TDP-43-eGFP into PLL3.7 backbone (acquired from Addgene).

Techniques:

Journal: Nature Neuroscience

Article Title: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models

doi: 10.1038/s41593-025-02062-6

Figure Lengend Snippet: Muscle derived CD-63 colocalizes and co-transports with axonal lysosomes . Representative live imaging video of muscle-derived CD63-GFP and lysotracker co-transport in axons within a co-culture with CD63-GFP expressing primary skeletal muscles. Magenta indicates lysotracker, green indicates CD63-GFP. Yellow arrowheads indicate co-transported CD-63 and lysotracker. Scale bar: 5 µm.

Article Snippet: CD63-peGFP C2 (plasmid no. 62964), pCMV-lyso-pHluorin (CD63-pHluorin; plasmid no. 70113) and pLKO-Tet-On-shRab27a (plasmid no. 120930) were acquired from Addgene repositories. pLL3.7-Ago2-eGFP was constructed in the Tel Aviv University lentiviral facility. pLL3.7-TDP-43-eGFP was constructed by cloning TDP-43-eGFP into PLL3.7 backbone (acquired from Addgene).

Techniques: