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cd63  (Novus Biologicals)
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Novus Biologicals cd63
(A) Characterization of the size distribution of the EVs isolated from GBM2 CTCs by Izon SEC using NTA. Measured total concentration: 0.9 × 10 9 particles/mL. (B) Characterization of the GBM2 EV protein content (CD9 and EGFR) using ELISA. (C) Characterization of the EV tetraspanin expression and distribution using ONI dSTORM imaging system. CD9‐AF488 (yellow dots), <t>CD63‐AF555</t> (blue dots) and CD81‐AF594 (pink dots) targeted and analysed. Right hand‐side image: zoom‐in on two EVs co‐expressing the three analysed tetraspanins. The numbers reported beside each tetraspanin represent the number of dots detected on each vesicle and are proportional to the amount/expression of that specific tetraspanin on the EV surface. (D) Validation of the EV capture onto a glass substrate. EVs labelled with tdTomato fluorophore were adsorbed overnight on a TB380 glass slide and imaged the day after, after several washing steps. Two different concentrations (Low EVs = [1×] = 3 × 10 7 particles/mL and High EVs = [8×] = 24 × 10 7 particles/mL) tested, and PSB used as a control. EV counts obtained from four FOVs and plotted on the right as mean ± SD. Images obtained with the 100× oil immersion lens (NA 1.45) of a widefield microscope (Nikon 90i) equipped with a cooled CCD camera (Andor Clara DR‐2519) and a 1.6× optical coupler (Nikon Digital Imaging Head).
Cd63, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd63 - by Bioz Stars, 2026-06
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(A) Characterization of the size distribution of the EVs isolated from GBM2 CTCs by Izon SEC using NTA. Measured total concentration: 0.9 × 10 9 particles/mL. (B) Characterization of the GBM2 EV protein content (CD9 and EGFR) using ELISA. (C) Characterization of the EV tetraspanin expression and distribution using ONI dSTORM imaging system. CD9‐AF488 (yellow dots), CD63‐AF555 (blue dots) and CD81‐AF594 (pink dots) targeted and analysed. Right hand‐side image: zoom‐in on two EVs co‐expressing the three analysed tetraspanins. The numbers reported beside each tetraspanin represent the number of dots detected on each vesicle and are proportional to the amount/expression of that specific tetraspanin on the EV surface. (D) Validation of the EV capture onto a glass substrate. EVs labelled with tdTomato fluorophore were adsorbed overnight on a TB380 glass slide and imaged the day after, after several washing steps. Two different concentrations (Low EVs = [1×] = 3 × 10 7 particles/mL and High EVs = [8×] = 24 × 10 7 particles/mL) tested, and PSB used as a control. EV counts obtained from four FOVs and plotted on the right as mean ± SD. Images obtained with the 100× oil immersion lens (NA 1.45) of a widefield microscope (Nikon 90i) equipped with a cooled CCD camera (Andor Clara DR‐2519) and a 1.6× optical coupler (Nikon Digital Imaging Head).

Journal: Journal of Extracellular Vesicles

Article Title: Signal Amplification for Fluorescent Staining of Single Particles in Liquid Biopsies: Circulating Tumour Cells and Extracellular Vesicles

doi: 10.1002/jev2.70167

Figure Lengend Snippet: (A) Characterization of the size distribution of the EVs isolated from GBM2 CTCs by Izon SEC using NTA. Measured total concentration: 0.9 × 10 9 particles/mL. (B) Characterization of the GBM2 EV protein content (CD9 and EGFR) using ELISA. (C) Characterization of the EV tetraspanin expression and distribution using ONI dSTORM imaging system. CD9‐AF488 (yellow dots), CD63‐AF555 (blue dots) and CD81‐AF594 (pink dots) targeted and analysed. Right hand‐side image: zoom‐in on two EVs co‐expressing the three analysed tetraspanins. The numbers reported beside each tetraspanin represent the number of dots detected on each vesicle and are proportional to the amount/expression of that specific tetraspanin on the EV surface. (D) Validation of the EV capture onto a glass substrate. EVs labelled with tdTomato fluorophore were adsorbed overnight on a TB380 glass slide and imaged the day after, after several washing steps. Two different concentrations (Low EVs = [1×] = 3 × 10 7 particles/mL and High EVs = [8×] = 24 × 10 7 particles/mL) tested, and PSB used as a control. EV counts obtained from four FOVs and plotted on the right as mean ± SD. Images obtained with the 100× oil immersion lens (NA 1.45) of a widefield microscope (Nikon 90i) equipped with a cooled CCD camera (Andor Clara DR‐2519) and a 1.6× optical coupler (Nikon Digital Imaging Head).

Article Snippet: The membranes were incubated overnight with primary antibodies (1:500 dilutions in 5% BSA with 0.02% sodium azide prepared in 1× TBST): HSP70 (cat. # 4872, Cell Signalling), CD63 (cat. # NB100‐77913, Novus Biologicals) and CD9 (cat. # 312102, BioLegend).

Techniques: Isolation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Imaging, Biomarker Discovery, Control, Microscopy

Comparison between DS, PSS and the developed TSA staining protocol using GBM2 EVs. (A, D, G) Representative images of the EVs stained using the three different techniques along with the respective control substrates (no EVs, PBS only + CD63 antibodies). (B, E, H) Fluorescent intensities (plotted as integrated intensities over all the pixels of each single EV) and counts of single EVs stained with the three different techniques, calculated considering two FOVs per technique. (C, F, I) Profiles of the EV pixel intensities over a 5‐min period of continuous laser excitation for the three analysed techniques. The four curves correspond to the pixel intensity distributions of the snapshot images captured at time 0, and after 1, 3 and 5 min of laser excitations, respectively.

Journal: Journal of Extracellular Vesicles

Article Title: Signal Amplification for Fluorescent Staining of Single Particles in Liquid Biopsies: Circulating Tumour Cells and Extracellular Vesicles

doi: 10.1002/jev2.70167

Figure Lengend Snippet: Comparison between DS, PSS and the developed TSA staining protocol using GBM2 EVs. (A, D, G) Representative images of the EVs stained using the three different techniques along with the respective control substrates (no EVs, PBS only + CD63 antibodies). (B, E, H) Fluorescent intensities (plotted as integrated intensities over all the pixels of each single EV) and counts of single EVs stained with the three different techniques, calculated considering two FOVs per technique. (C, F, I) Profiles of the EV pixel intensities over a 5‐min period of continuous laser excitation for the three analysed techniques. The four curves correspond to the pixel intensity distributions of the snapshot images captured at time 0, and after 1, 3 and 5 min of laser excitations, respectively.

Article Snippet: The membranes were incubated overnight with primary antibodies (1:500 dilutions in 5% BSA with 0.02% sodium azide prepared in 1× TBST): HSP70 (cat. # 4872, Cell Signalling), CD63 (cat. # NB100‐77913, Novus Biologicals) and CD9 (cat. # 312102, BioLegend).

Techniques: Comparison, Staining, Control