Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

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Article Snippet: anti-human CD57 -176Yb , Fluidigm , Cat# 3176019B.

Techniques: Recombinant, Antibody Labeling, Enzyme-linked Immunosorbent Assay, Sequencing, Software

Journal: Cell Reports Medicine

Article Title: Response and recurrence correlates in individuals treated with neoadjuvant anti-PD-1 therapy for resectable oral cavity squamous cell carcinoma

doi: 10.1016/j.xcrm.2021.100411

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Article Snippet: CD57 (HCD57) , Fluidigm , Cat# 3172009B, RRID: AB_2888930.

Techniques: Recombinant, Isolation, Mass Cytometry, Software

Journal: NPJ precision oncology

Article Title: CD57-positive CD8 + T cells define the response to anti-programmed cell death protein-1 immunotherapy in patients with advanced non-small cell lung cancer.

doi: 10.1038/s41698-024-00513-0

Figure Lengend Snippet: Fig. 4 mIHC/IF-based quantification of CD57+CD8+ T cells predicts immunotherapeutic response in a retrospective NSCLC cohort (n = 90). a Representative images of NSCLC tissue stained using mIHC/IF [CD3 (green), CD8 (red), CD57 (pink), DAPI (blue)]. b mIHC/IF-based CD57+CD8+ T cells/T cells ratio (p < 0.001) and (c) CD57+CD8+ T cells/CD8+ T cells ratio (p < 0.001) predicted immunotherapeutic response in a retrospective NSCLC cohort (n = 90). d Total CD8+ T cells/T cells ratio by mIHC/IF was not of predictive value to immunotherapy (p = 0.679, n = 90). e Spearman’s rank correlation analysis was performed to compare the CD57+CD8+ T cells/T cells ratio (r = 0.644, p < 0.001), and f CD8+ T cells/T cells ratio (r = 0.346, p = 0.016) in blood and tumor samples (n = 48). ***p < 0.001. mIHC/IF multiplex immunohistochemistry/ immunofluorescence, NSCLC non-small cell lung cancer.

Article Snippet: Briefly, each FFPE tissue section was baked at 65 °C for 1 h. After deparaffinization, rehydration, and microwave antigen repair, the slides were blocked (Akoya Biosciences, USA), and incubated with primary antibodies against CD3 (D7A6E, CST, 85061 T, 1:200), CD57 (HNK-1, CST, 72031 S, 1:200), and CD8 (EPR22483-288, Abcam, ab245118, 1:500), respectively, followed by incubation with Opal Polymer HRP Ms+Rb (Akoya Biosciences, USA).

Techniques: Staining, Multiplex Assay, Immunohistochemistry

Journal: iScience

Article Title: Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches.

doi: 10.1016/j.isci.2023.107261

Figure Lengend Snippet: Figure 1. Assessment of the tonsillar cellular composition with multiparametric flow cytometry (A) The gating scheme for the flow cytometry analysis of relevant cell subsets is shown (upper panel). The relative expression (intensity) of CD38, PD1 and CD57 in tonsillar CD4 (red) and CD8 (green) subsets is shown (lower panel). The overlay of corresponding 2D flow cytometry plots visualizes the different level of expression between the two T cell subsets with TFH cells expressing the highest level of PD1. (B) Bar graphs showing accumulated data regarding the relative frequency of B, CD4 and CD8 subsets in tonsils (n = 7) analyzed. (C) Significant correlation between GC B cells and TFH subsets and GC B cells and plasma cells. Linear regression was used for the analysis.

Article Snippet: The following titrated, conjugated antibodies were used for flow cytometry analysis: CD3-H7APC (SK7, BD Biosciences), CD4-BV650 (SK3, BD Biosciences), CD8-Pacific Blue (RPA-T8, BD Pharmingen), CD19-FITC (J3-119, Beckman-Coulter), CD27-BV605 (O323, Biolegend), CD45RO-Cy5PE (UCHL1, BD Pharmingen), PD-1-BV711 (EH12.2H7, Biolegend), CD57-AF594 (NK-1, Novus Bio), IgD-PE (Goat polyclonal, Southern Biotech), CD20-BV570 (2H7, Biolegend), CD38-BV786 (HIT2, Biolegend).

Techniques: Cytometry, Expressing, Clinical Proteomics

Journal: iScience

Article Title: Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches.

doi: 10.1016/j.isci.2023.107261

Figure Lengend Snippet: Figure 2. Compartmentalization of tonsillar cell types and soluble mediators revealed by multiplex imaging analysis (A) Representative hematoxylin and eosin (H&E) stained human tonsil section. Relevant anatomical segments are labeled for tonsillar crypt (C), germinal center (GC), mantle zone (MZ), crypt epithelium (E), T cell zone (TZ) and extrafollicular area (EF). (B) Representative multiplex confocal images showing the distribution of B, CD4 and CD8 cell subsets in a follicular area and adjacent ‘T cell area’. Zoomed areas (white boxes) are also shown for the visualization of the expression of relevant biomarkers on individual cells. Scale bars top row are 100 mm. (C) Bar graphs showing the relative expression (as frequency of the corresponding parental population) of Ki67+ B cells and PD-1+CD57+ CD4 T cells as well as the frequency (of total cells) of CD8 and CD68 subsets in different tonsillar areas (EF = extrafollicular, MZ = mantle zone, GC = germinal center) (upper panel), Kruskal-Wallis (G SEM) statistic test used significant differences notated. The correlation between Ki67+ GC B cells and PD-1+CD57+ CD4 TFH cells is shown (lower panel), Simple linear regression statistical test used. (D) Representative multispectral images showing the distribution of FDCs (green), CD14 (yellow), CXCL13 (red) and IL17 (cyan) with respect to the follicular areas (CD20, blue) in one tonsil. Inserts are showing the expression of IL17 in zoomed areas (white circles). Scale bars are 200 mm.

Article Snippet: The following titrated, conjugated antibodies were used for flow cytometry analysis: CD3-H7APC (SK7, BD Biosciences), CD4-BV650 (SK3, BD Biosciences), CD8-Pacific Blue (RPA-T8, BD Pharmingen), CD19-FITC (J3-119, Beckman-Coulter), CD27-BV605 (O323, Biolegend), CD45RO-Cy5PE (UCHL1, BD Pharmingen), PD-1-BV711 (EH12.2H7, Biolegend), CD57-AF594 (NK-1, Novus Bio), IgD-PE (Goat polyclonal, Southern Biotech), CD20-BV570 (2H7, Biolegend), CD38-BV786 (HIT2, Biolegend).

Techniques: Multiplex Assay, Imaging, Staining, Labeling, Expressing