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Image Search Results
Journal: Cancers
Article Title: CAR-NK Cells Targeting HER1 (EGFR) Show Efficient Anti-Tumor Activity against Head and Neck Squamous Cell Carcinoma (HNSCC)
doi: 10.3390/cancers15123169
Figure Lengend Snippet: Characterization of primary HNSCC (pHNSCC) cells. Characterization of ( A ) CD45 + immune cells present in isolated pHNSCC samples as well as ( B ) percentage of NK cells (CD56 + ), macrophages (CD11b + ), B cells (CD19 + ) and T cells (CD3 + ) of CD45 + cells in pHNSCC samples and ( C ) percentages of stroma cells (CD140a + ), endothelial cells (CD31 + ), cancer-associated fibroblasts (FAP + ) and epithelial marker-positive cells (EpCAM + ) in the CD45 − pHNSCC cell population within 24 h from isolation (or thawing). ( D ) Target antigen expression within 24 h of isolation (or thawing) of pHNSCC samples. ( E ) Quantitative analysis of immunohistochemical stainings of seven patient samples (photos from exemplary patient samples shown in ). ( F ) HER1 expression on EpCAM + sorted and expanded pHNSCC samples. Optical genome mapping of EpCAM + sorted and expanded pHNSCC samples ( G ) #14 and ( H ) #16. Red box highlights amplification of 11q13, (Legend: ● copy number gains (blue lines), ● copy number losses (red lines), ● insertions, ● deletions, ● inversions, ● duplications, ● intra-fusions or inter-translocations (pink lines)). Mean with standard deviation shown. n = 4–9 pHNSCC samples as indicated ( A – E ).
Article Snippet: Next, cells were incubated with anti-Flag PE (BioLegend, San Diego, CA, USA) and
Techniques: Isolation, Marker, Expressing, Immunohistochemical staining, Amplification, Standard Deviation